RNA interference mediated inhibition of epidermal growth factor receptor gene expression using short interfering nucleic acid (siNA)

ABSTRACT

The present invention concerns methods and reagents useful in modulating EGFR (HER1, HER2, HER3, and/or HER4) gene expression in a variety of applications, including use in therapeutic, diagnostic, agricultural, target validation, and genomic discovery applications. Specifically, the invention relates to short interfering nucleic acid (siNA) or short interfering RNA (siRNA) molecules capable of mediating RNA interference (RNAi) against epidermal growth factor receptor targets.

PRIORITY

[0001] This application claims the benefit of U.S. application Ser. Nos. 60/358,580, filed Feb. 20, 2002, and 60/393,924, filed Jul. 3, 2002. This application also claims priority to U.S. application Ser. No. 09/916,466, filed Jul. 25, 2001 and to U.S. application Ser. No. 10/163,552, filed Jun. 6, 2002, which claims the benefit of U.S. application Ser. No. 60/296,249, filed Jun. 6, 2001.

BACKGROUND OF THE INVENTION

[0002] The present invention concerns methods and reagents useful in modulating epidermal growth factor receptor (EGFR) gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to short interfering nucleic acid molecules (siNA) capable of mediating RNA interference (RNAi) against HER1, HER2, HER3 and HER4 expression.

[0003] The following is a discussion of relevant art pertaining to RNAi. The discussion is provided only for understanding of the invention that follows. The summary is not an admission that any of the work described below is prior art to the claimed invention.

[0004] RNA interference refers to the process of sequence-specific post-transcriptional gene silencing in animals mediated by short interfering RNAs (siRNAs) (Fire et al., 1998, Nature, 391, 806). The corresponding process in plants is commonly referred to as post-transcriptional gene silencing or RNA silencing and is also referred to as quelling in fungi. The process of post-transcriptional gene silencing is thought to be an evolutionarily-conserved cellular defense mechanism used to prevent the expression of foreign genes which is commonly shared by diverse flora and phyla (Fire et al., 1999, Trends Genet., 15, 358). Such protection from foreign gene expression may have evolved in response to the production of double-stranded RNAs (dsRNAs) derived from viral infection or from the random integration of transposon elements into a host genome via a cellular response that specifically destroys homologous single-stranded RNA or viral genomic RNA. The presence of dsRNA in cells triggers the RNAi response though a mechanism that has yet to be fully characterized. This mechanism appears to be different from the interferon response that results from dsRNA-mediated activation of protein kinase PKR and 2′,5′-oligoadenylate synthetase resulting in non-specific cleavage of mRNA by ribonuclease L.

[0005] The presence of long dsRNAs in cells stimulates the activity of a ribonuclease III enzyme referred to as dicer. Dicer is involved in the processing of the dsRNA into short pieces of dsRNA known as short interfering RNAs (siRNAs) (Berstein et al., 2001, Nature, 409, 363). Short interfering RNAs derived from dicer activity are typically about 21-23 nucleotides in length and comprise about 19 base pair duplexes. Dicer has also been implicated in the excision of 21 and 22 nucleotide small temporal RNAs (stRNAs) from precursor RNA of conserved structure that are implicated in translational control (Hutvagner et al., 2001, Science, 293, 834). The RNAi response also features an endonuclease complex, commonly referred to as an RNA-induced silencing complex (RISC), which mediates cleavage of single-stranded RNA having sequence complementary to the antisense strand of the siRNA duplex. Cleavage of the target RNA takes place in the middle of the region complementary to the antisense strand of the siRNA duplex (Elbashir et al., 2001, Genes Dev., 15, 188).

[0006] Short interfering RNA mediated RNAi has been studied in a variety of systems. Fire et al., 1998, Nature, 391, 806, were the first to observe RNAi in C. elegans. Wianny and Goetz, 1999, Nature Cell Biol., 2, 70, describe RNAi mediated by dsRNA in mouse embryos. Hammond et al., 2000, Nature, 404, 293, describe RNAi in Drosophila cells transfected with dsRNA. Elbashir et al., 2001, Nature, 411, 494, describe RNAi induced by introduction of duplexes of synthetic 21-nucleotide RNAs in cultured mammalian cells including human embryonic kidney and HeLa cells. Recent work in Drosophila embryonic lysates (Elbashir et al., 2001, EMBO J, 20, 6877) has revealed certain requirements for siRNA length, structure, chemical composition, and sequence that are essential to mediate efficient RNAi activity. These studies have shown that 21-nucleotide siRNA duplexes are most active when containing 3′-terminal di-nucleotide overhangs. Furthermore, complete substitution of one or both siRNA strands with 2′-deoxy (2′-H) or 2′-O-methyl nucleotides abolishes RNAi activity, whereas substitution of the 3′-terminal siRNA overhang nucleotides with deoxy nucleotides (2′-H) was shown to be tolerated. Single mismatch sequences in the center of the siRNA duplex were also shown to abolish RNAi activity. In addition, these studies also indicate that the position of the cleavage site in the target RNA is defined by the 5′-end of the siRNA guide sequence rather than the 3′-end o of the siRNA guide sequence (Elbashir et al., 2001, EMBO J, 20, 6877). Other studies have indicated that a 5′-phosphate on the target-complementary strand of a siRNA duplex is required for siRNA activity and that ATP is utilized to maintain the 5′-phosphate moiety on the siRNA (Nykanen et al., 2001, Cell, 107, 309).

[0007] Studies have shown that replacing the 3′-terminal nucleotide overhanging segments of a 21-mer siRNA duplex having two 2 nucleotide 3′-overhangs with deoxyribonucleotides does not have an adverse effect on RNAi activity. Replacing up to 4 nucleotides on each end of the siRNA with deoxyribonucleotides has been reported to be well tolerated whereas complete substitution with deoxyribonucleotides results in no RNAi activity (Elbashir et al., 2001, EMBO J., 20, 6877). In addition, Elbashir et al., supra, also report that substitution of siRNA with 2′-O-methyl nucleotides completely abolishes RNAi activity. Li et al., International PCT Publication No. WO 00/44914, and Beach et al, International PCT Publication No. WO 01/68836, both suggest that siRNA “may include modifications to either the phosphate-sugar back bone or the nucleoside . . . to include at least one of a nitrogen or sulfur heteroatom”, however, neither application teaches to what extent these modifications are tolerated in siRNA molecules nor provides any examples of such modified siRNA. Kreutzer and Limmer, Canadian Patent Application No. 2,359,180, also describe certain chemical modifications for use in dsRNA constructs in order to counteract activation of double-stranded-RNA-dependent protein kinase PKR, specifically 2′-amino or 2′-O-methyl nucleotides, and nucleotides containing a 2′-O or 4′-C methylene bridge. However, Kreutzer and Limmer similarly fail to show to what extent these modifications are tolerated in siRNA molecules nor do they provide any examples of such modified siRNA.”

[0008] Parrish et al., 2000, Molecular Cell, 6, 1977-1087, tested certain chemical modifications targeting the unc-22 gene in C. elegans using long (>25 nt) siRNA transcripts. The authors describe the introduction of thiophosphate residues into these siRNA transcripts by incorporating thiophosphate nucleotide analogs with T7 and T3 RNA polymerase and observed that “RNAs with two [phosphorothioate] modified bases also had substantial decreases in effectiveness as RNAi triggers (data not shown); [phosphorothioate] modification of more than two residues greatly destabilized the RNAs in vitro and we were not able to assay interference activities.” Id. at 1081. The authors also tested certain modifications at the 2′-position of the nucleotide sugar in the long siRNA transcripts and observed that substituting deoxynucleotides for ribonucleotides “produced a substantial decrease in interference activity,” especially in the case of Uridine to Thymidine and/or Cytidine to deoxy-Cytidine substitutions. Id. In addition, the authors tested certain base modifications, including substituting in sense and antisense strands of the siRNA, 4-thiouracil, 5-bromouracil, 5-iodouracil, 3-(aminoallyl)uracil for uracil, and inosine for guanosine. They found that whereas 4-thiouracil and 5-bromouracil were all well tolerated, inosine “produced a substantial decrease in interference activity” when incorporated in either strand. Incorporation of 5-iodouracil and 3-(aminoallyl)uracil in the antisense strand resulted in substantial decrease in RNAi activity as well.

[0009] Beach et al., International PCT Publication No. WO 01/68836, describe specific methods for attenuating gene expression using endogenously derived dsRNA. Tuschl et al., International PCT Publication No. WO 01/75164, describe a Drosophila in vitro RNAi system and the use of specific siRNA molecules for certain functional genomic and certain therapeutic applications; although Tuschl, 2001, Chem. Biochem., 2, 239-245, doubts that RNAi can be used to cure genetic diseases or viral infection due “to the danger of activating interferon response”. Li et al., International PCT Publication No. WO 00/44914, describes the use of specific dsRNAs for use in attenuating the expression of certain target genes. Zernicka-Goetz et al., International PCT Publication No. WO 01/36646, describe certain methods for inhibiting the expression of particular genes in mammalian cells using certain dsRNA molecules. Fire et al., International PCT Publication No. WO 99/32619, describe particular methods for introducing certain dsRNA molecules into cells for use in inhibiting gene expression. Plaetinck et al., International PCT Publication No. WO 00/01846, describe certain methods for identifying specific genes responsible for conferring a particular phenotype in a cell using specific dsRNA molecules. Mello et al., International PCT Publication No. WO 01/29058, describe the identification of specific genes involved in dsRNA mediated RNAi. Deschamps Depaillette et al., International PCT Publication No. WO 99/07409, describe specific compositions consisting of particular dsRNA molecules combined with certain anti-viral agents. Waterhouse et al., International PCT Publication No. 99/53050, describe certain methods for decreasing the phenotypic expression of a nucleic acid in plant cells. Driscoll et al., International PCT Publication No. WO 01/49844, describe specific DNA constructs for use in facilitating gene silencing in targeted organisms. Parrish et al., 2000, Molecular Cell, 6, 1977-1087, describe specific chemically-modified siRNA constructs targeting the unc-22 gene of C. elegans. Grossniklaus, International PCT Publication No. WO 01/38551, describes certain methods for regulating polycomb gene expression in plants. Churikov et al., International PCT Publication No. WO 01/42443, describe certain methods for modifying genetic characteristics of an organism. Cogoni et al., International PCT Publication No. WO 01/53475, describe certain methods for isolating a Neurospora silencing gene and uses thereof. Reed et al., International PCT Publication No. WO 01/68836, describe certain methods for gene silencing in plants. Honer et al., International PCT Publication No. WO 01/70944, describe certain methods of drug screening using transgenic nematodes as Parkinson's disease models. Deak et al., International PCT Publication No. WO 01/72774, describe certain Drosophila derived gene products. Arndt et al., International PCT Publication No. WO 01/92513 describe certain methods for mediating gene suppression by using factors that enhance RNAi. Tuschl et al., International PCT Publication No. WO 02/44321, describe certain synthetic siRNA constructs. Pachuk et al., International PCT Publication No. WO 00/63364, and Satishchandran et al., International PCT Publication No. WO 01/04313 describe certain methods and compositions for inhibiting the function of certain polynucleotide sequences. Echeverri et al., International PCT Publication No. WO 02/38805, describe certain C. elegans genes identified via RNAi. Kreutzer et al., International PCT Publication Nos. WO 02/055692 and WO 02/055693, describe certain methods for inhibiting gene expression using RNAi.

[0010] The epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein consisting of an extracellular ‘ligand’ binding domain, a transmembrane region and an intracellular domain with tyrosine kinase activity (Kung et al., 1994). The binding of growth factors to the EGFR results in down regulation of the ligand-receptor complex, autophosphorylation of the receptor and other protein substrates, leading ultimately to DNA synthesis and cell division. The external ligand binding domain is stimulated by EGF and also by TGFa, amphiregulin and some viral growth factors (Modjtahedi & Dean, 1994).

[0011] One of the striking characteristics of the EGFR gene (c-erbB1), located on chromosome 7, is it's homology to the avian erythroblastosis virus oncogene (v-erbB), which induces malignancies in chickens. The v-erbB gene codes for a truncated product that lacks the extracellular ligand binding domain. The tyrosine kinase domain of the EGFR has been found to have 97% homology to the v-erbB transforming protein (Downward et al., 1984).

[0012] Recent studies have shown that the EGFR is overexpressed in a number of malignant human tissues when compared to their normal tissue counterparts (for review see Khazaie et al., 1993). An important finding has been the discovery that the gene for the receptor is both amplified and overexpressed in a number of cancer cells. Overexpression of the EGFR is often accompanied by the co-expression of the growth factors EGF and TGF∝, suggesting that an autocrine pathway for control of growth may play a major part in the progression of tumors (Sporn & Roberts, 1985). It is now widely believed that this is a mechanism by which tumor cells can escape normal physiological control.

[0013] Growth factors and their receptors appear to have an important role in the development of human brain tumors. A high incidence of overexpression, amplification, deletion and structural rearrangement of the gene coding for the EGFR has been found in biopsies of brain tumors (Ostrowski et al., 1994). In fact the amplification of the EGFR gene in glioblastoma multiforme tumors is one of the most consistent genetic alterations known, with the EGFR being overexpressed in approximately 40% of malignant gliomas (Black, 1991). It has also been demonstrated that in 50% of glioblastomas, amplification of the EGFR gene is accompanied by the co-expression of mRNA for at least one or both of the growth factors EGF and TNFα (Ekstrand et al., 1991).

[0014] The amplified genes are frequently rearranged and associated with polymorphism leading to abnormal protein products (Wong et al., 1994). The rearrangements that have been characterized usually show deletions of part of the extracellular domain, resulting in the production of an EGFR protein that is smaller in size. Three classes of deletion mutant EGF receptor genes have been identified in glioblastoma tumors. Type I mutants lack the majority of the external domain, including the ligand binding site, type II mutants have a deletion in the domain adjacent to the membrane but can still bind ligands and type III, which is the most common and found in 17% of glioblastomas, have a deletion of 267 amino acids spanning domains I and II of the EGFR.

[0015] In addition to glioblastomas, abnormal EGFR expression has also been reported in a number of squamous epidermoid cancers and breast cancers (reviewed in Kung et al, 1994; Modjtahedi & Dean, 1994). Interestingly, evidence also suggests that many patients with tumors that over-express the EGFR have a poorer prognosis than those who do not (Khazaie et al., 1993). Consequently, therapeutic strategies which can potentially inhibit or reduce the aberrant expression of the EGFR receptor are of great interest as potential anti-cancer agents.

SUMMARY OF THE INVENTION

[0016] This invention relates to compounds, compositions, and methods useful for modulating epidermal growth factor receptor (EGFR) function and/or gene expression in a cell by RNA interference (RNAi) using short interfering nucleic acid (siNA). In particular, the instant invention features siNA molecules and methods to modulate the expression of an epidermal growth factor receptor (EGFR), such as HER1, HER2, HER3 and HER4. A siNA of the invention can be unmodified or chemically-modified. A siNA of the instant invention can be chemically synthesized, expressed from a vector or enzymatically synthesized. The instant invention also features various chemically-modified synthetic short interfering nucleic acid (siNA) molecules capable of modulating EGFR gene expression in cells by RNA inference (RNAi). The use of chemically-modified siNA is expected to improve various properties of native siNA molecules through increased resistance to nuclease degradation in vivo and/or improved cellular uptake. The siNA molecules of the instant invention provide useful reagents and methods for a variety of therapeutic, diagnostic, agricultural, target validation, genomic discovery, genetic engineering and pharmacogenomic applications.

[0017] In one embodiment, the invention features one or more siNA molecules and methods that independently or in combination modulate the expression of gene(s) encoding epidermal growth factor receptors. Specifically, the present invention features siNA molecules that modulate the expression of EGFR genes HER1 (for example Genbank Accession No. NM_(—)005228), HER2 (erbB2/neu) (for example Genbank Accession No. NM_(—)004448), HER3 (for example Genbank Accession No. NM_(—)001982), and HER4 (for example Genbank Accession No. NM_(—)005235).

[0018] The description below of the various aspects and embodiments is provided with reference to the exemplary epidermal growth receptor (EGFR) genes HER1, HER2, HER3, and HER4, collectively referred to hereinafter as EGFR. However, the various aspects and embodiments are also directed to other genes which express EGFR proteins and other receptors involved in oncogenesis. Those additional genes can be analyzed for target sites using the methods described for EGFR. Thus, the inhibition and the effects of such inhibition of the other genes can be performed as described herein.

[0019] In one embodiment, the invention features a siNA molecule that down regulates expression of an epidermal growth factor receptor (EGFR) gene by RNA interference. The EGFR gene can comprise, for example, HER1 sequence, HER2 sequence, HER3 sequence, or HER4 sequence and/or any combination thereof.

[0020] In one embodiment, the invention features a siNA molecule having RNAi activity against HER2 RNA, wherein the siNA molecule comprises a sequence complementary to any RNA having HER2 encoding sequence, for example Genbank Accession No. NM_(—)004448. In another embodiment, the invention features a siNA molecule comprising a sequence selected from the group consisting of SEQ ID NOs: 1-552 and 1187-1204. The sequences shown in SEQ ID NOs: 1-552 and 1187-1204 are not limiting. A siNA molecule of the invention can comprise any contiguous HER2 sequences (e.g., about 19 contiguous HER2 nucleotides. In another embodiment, the invention features a siNA molecule having RNAi activity against HER1 RNA, wherein the siNA molecule comprises a sequence complementary to any RNA having HER1 encoding sequence, for example Genbank Accession No. NM_(—)005228. In another embodiment, the invention features a siNA molecule comprising a sequence selected from the group consisting of SEQ ID NOs: 553-1186, 1187-1195, and 1205-1213. The sequences shown in SEQ ID NOs: 553-1186, 1187-1195, and 1205-1213 are not limiting. A siNA molecule of the invention can comprise any contiguous HER1 sequences (e.g., about 19 contiguous HER1 nucleotides. In yet another embodiment, the invention features a siNA molecule comprising a sequence complementary to a sequence comprising Genbank Accession Nos. NM_(—)005228 (HER1), NM_(—)004448 (HER2), NM_(—)001982 (HER3), and/or NM_(—)005235 (HER4).

[0021] In one embodiment, a siNA molecule of the invention has RNAi activity that modulates expression of RNA encoded by an EGFR gene, for example, a HER1, HER2, HER3, or HER4 gene and any combination thereof.

[0022] In one embodiment of the invention a siNA molecule is adapted for use to treat cancer. A siNA molecule can comprise a sense region and an antisense region, wherein said antisense region can comprise sequence complementary to an RNA sequence encoding EGFR and the sense region can comprise sequence complementary to the antisense region. A siNA molecule can be assembled from two nucleic acid fragments wherein one fragment can comprise the sense region and the second fragment can comprise the antisense region of said siNA molecule. The sense region and antisense region can be covalently connected via a linker molecule. The linker molecule can be a polynucleotide or non-nucleotide linker. The sense region of a siNA molecule of the invention can comprise a 3′-terminal overhang and the antisense region can comprise a 3′-terminal overhang. The 3′-terminal overhangs each can comprise about 2 nucleotides. The antisense region 3′-terminal nucleotide overhang can be complementary to RNA encoding EGFR. The sense region can comprise a terminal cap moiety at the 5′-end, 3′-end, or both 5′ and 3′ ends of the sense region.

[0023] In one embodiment, nucleic acid molecules of the invention that act as mediators of the RNA interference gene silencing response are double-stranded RNA molecules. In another embodiment, the siNA molecules of the invention consist of duplexes containing about 19 base pairs between oligonucleotides comprising about 19 to about 25 nucleotides. In yet another embodiment, siNA molecules of the invention comprise duplexes with overhanging ends of about 1 to about 3 nucleotides, for example about 21 nucleotide duplexes with about 19 base pairs and about 2 nucleotide 3′-overhangs.

[0024] In one embodiment, the invention features one or more chemically-modified siNA constructs having specificity for EGFR expressing nucleic acid molecules. Non-limiting examples of such chemical modifications include without limitation phosphorothioate internucleotide linkages, 2′-O-methyl ribonucleotides, 2′-O-methyl modified pyrimidine nucleotides, 2′-deoxy-2′-fluoro ribonucleotides, 2′-deoxy-2-fluoro modified pyrimidine nucleotides, “universal base” nucleotides, 5-C-methyl nucleotides, and inverted deoxy abasic residue incorporation. These chemical modifications, when used in various siNA constructs, are shown to preserve RNAi activity in cells while at the same time, dramatically increasing the serum stability of these compounds. Furthermore, contrary to the data published by Parrish et al., supra, applicant demonstrates that multiple (greater than one) phosphorothioate substitutions are well tolerated and confer substantial increases in serum stability for modified siNA constructs.

[0025] The antisense region of a siNA molecule of the invention can comprise a phosphorothioate internucleotide linkage at the 3′ end of said antisense region. The antisense region can comprise between about one and about five phosphorothioate internucleotide linkages at the 5′ end of said antisense region. The 3′-terminal nucleotide overhangs of a siNA molecule of the invention can comprise ribonucleotides or deoxyribonucleotides that are chemically-modified at a nucleic acid sugar, base, or backbone. The 3′-terminal nucleotide overhangs can comprise one or more universal base ribonucleotides. The 3′-terminal nucleotide overhangs can comprise one or more acyclic nucleotides.

[0026] In a non-limiting example, the introduction of chemically-modified nucleotides into nucleic acid molecules will provide a powerful tool in overcoming potential limitations of in vivo stability and bioavailability inherent to native RNA molecules that are delivered exogenously. For example, the use of chemically-modified nucleic acid molecules can enable a lower dose of a particular nucleic acid molecule for a given therapeutic effect since chemically-modified nucleic acid molecules tend to have a longer half-life in serum. Furthermore, certain chemical modifications can improve the bioavailability of nucleic acid molecules by targeting particular cells or tissues and/or improving cellular uptake of the nucleic acid molecule. Therefore, even if the activity of a chemically-modified nucleic acid molecule is reduced as compared to a native nucleic acid molecule, for example when compared to an all RNA nucleic acid molecule, the overall activity of the modified nucleic acid molecule can be greater than the native molecule due to improved stability and/or delivery of the molecule. Unlike native unmodified siNA, chemically-modified siNA can also minimize the possibility of activating interferon activity in humans.

[0027] One embodiment of the invention provides an expression vector comprising a nucleic acid sequence encoding at least one siNA molecule of the invention in a manner that allows expression of the nucleic acid molecule. Another embodiment of the invention provides a mammalian cell comprising such an expression vector. The mammalian cell can be a human cell. The siNA molecule of the expression vector can comprise a sense region and an antisense region and the antisense region can comprise sequence complementary to a RNA sequence encoding EGFR and the sense region can comprise sequence complementary to the antisense region. The siNA molecule can comprise two distinct strands having complementarity sense and antisense regions. The siNA molecule can comprise a single-strand having complementary sense and antisense regions.

[0028] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein the chemical modification comprises one or more nucleotides comprising a backbone modified internucleotide linkage, for example, at a 3′ terminal nucleotide overhang, having Formula I:

[0029] wherein each R1 and R2 is independently any nucleotide, non-nucleotide, or polynucleotide which can be naturally occurring or chemically-modified, each X and Y is independently O, S, N, alkyl, or substituted alkyl, each Z and W is independently O, S, N, alkyl, substituted alkyl, O-alkyl, S-alkyl, alkaryl, or aralkyl.

[0030] The chemically-modified internucleotide linkages having Formula I, for example wherein any Z, W, X, and/or Y independently comprises a sulphur atom, can be present in one or both oligonucleotide strands of the siNA duplex, for example in the sense strand, the antisense strand, or both strands. The siNA molecules of the invention can comprise one or more chemically-modified internucleotide linkages having Formula I at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense strand, the antisense strand, or both strands. For example, an exemplary siNA molecule of the invention can comprise between about 1 and about 5 chemically-modified internucleotide linkages having Formula I at the 5′-end of the sense strand, the antisense strand, or both strands. In another non-limiting example, an exemplary siNA molecule of the invention can comprise one or more pyrimidine nucleotides with chemically-modified internucleotide linkages having Formula I in the sense strand, the antisense strand, or both of the strands. In yet another non-limiting example, an exemplary siNA molecule of the invention can comprise one or more purine nucleotides with chemically-modified internucleotide linkages having Formula I in the sense strand, antisense strand, or both strands. In another embodiment, a siNA molecule of the invention having internucleotide linkage(s) of Formula I also comprises a chemically-modified nucleotide or non-nucleotide having any of Formulae II, III, V, VI, or VII.

[0031] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein the chemical modification comprises one or more nucleotides or non-nucleotides at, for example, a 3′ terminal nucleotide overhang, having Formula II:

[0032] wherein each R3, R4, R5, R6, R7, R8, R10, R 11 and R12 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, Cl, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO2, NO2, N3, NH2, aminoalkyl, aminoacid, aminoacyl, ONH2, O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I; R9 is O, S, CH2, S═O, CHF, or CF2, and B is a nucleosidic base such as adenine, guanine, uracil, cytosine, thymine, 2-aminoadenosine, 5-methylcytosine, 2,6-diaminopurine, or any other non-naturally occurring base that can be employed to form a stable duplex with RNA or a non-nucleosidic base such as phenyl, naphthyl, 3-nitropyrrole, 5-nitroindole, nebularine, pyridone, pyridinone, or any other non-naturally occurring universal base that can be employed to form a stable duplex with RNA.

[0033] The chemically-modified nucleotide or non-nucleotide of Formula II can be present in one or both oligonucleotide strands of the siNA duplex, for example in the sense strand, the antisense strand, or both strands. The siNA molecules of the invention can comprise one or more chemically-modified nucleotide or non-nucleotide of Formula II at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense strand, antisense strand, or both strands. For example, an exemplary siNA molecule of the invention can comprise between about 1 and about 5 chemically-modified nucleotide or non-nucleotide of Formula II at the 5′-end of the sense strand, the antisense strand, or both of the strands. In anther non-limiting example, an exemplary siNA molecule of the invention can comprise between about 1 and about 5 chemically-modified nucleotide or non-nucleotide of Formula II at the 3′-end of the sense strand, the antisense strand, or both strands.

[0034] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein the chemical modification comprises one or more nucleotides or non-nucleotides having Formula III:

[0035] wherein each R3, R4, R5, R6, R7, R8, R10, R11 and R12 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, Cl, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO2, NO2, N3, NH2, aminoalkyl, aminoacid, aminoacyl, ONH2, O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I; R9 is O, S, CH2, S═O, CHF, or CF2, and B is a nucleosidic base such as adenine, guanine, uracil, cytosine, thymine, 2-aminoadenosine, 5-methylcytosine, 2,6-diaminopurine, or any other non-naturally occurring base that can be employed to form a stable duplex with RNA or a non-nucleosidic base such as phenyl, naphthyl, 3-nitropyrrole, 5-nitroindole, nebularine, pyridone, pyridinone, or any other non-naturally occurring universal base that can be employed to form a stable duplex with RNA.

[0036] The chemically-modified nucleotide or non-nucleotide of Formula III can be present in one or both oligonucleotide strands of the siNA duplex, for example in the sense strand, the antisense strand, or both strands. The siNA molecules of the invention can comprise one or more chemically-modified nucleotide or non-nucleotide of Formula III at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense strand, the antisense strand, or both strands. For example, an exemplary siNA molecule of the invention can comprise between about 1 and about 5 chemically-modified nucleotide or non-nucleotide of Formula III at the 5′-end of the sense strand, the antisense strand, or both strands. In anther non-limiting example, an exemplary siNA molecule of the invention can comprise between about 1 and about 5 chemically-modified nucleotide or non-nucleotide of Formula III at the 3′-end of the sense strand, the antisense strand, or both strands.

[0037] In another embodiment, a siNA molecule of the invention comprises a nucleotide having Formulae II or III, wherein the nucleotide having Formulae II or III is in an inverted configuration. For example, the nucleotide having Formulae II or III is connected to the siNA construct in a 3′-3′, 3′-2′, 2′-3′, or 5′-5′ configuration, such as at the 3′-end, the 5′-end, or both of the 3′ and 5′ ends of one or both siNA strands.

[0038] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein the chemical modification comprises a 5′-terminal phosphate group having Formula IV:

[0039] wherein each X and Y is independently O, S, N, alkyl, substituted alkyl, or alkylhalo; each Z and W is independently O, S, N, alkyl, substituted alkyl, O-alkyl, S-alkyl, alkaryl, aralkyl, or alkylhalo; and wherein W, X, Y and Z are not O.

[0040] In one embodiment, the invention features a siNA molecule having a 5′-terminal phosphate group having Formula IV on the target-complementary strand, for example a strand complementary to EGFR RNA, wherein the siNA molecule comprises an all RNA siNA molecule. In another embodiment, the invention features a siNA molecule having a 5′-terminal phosphate group having Formula IV on the target-complementary strand wherein the siNA molecule also comprises 1-3 nucleotide 3′-terminal nucleotide overhangs having between about 1 and about 4 deoxyribonucleotides on the 3′-end of one or both strands. In another embodiment, a 5′-terminal phosphate group having Formula IV is present on the target-complementary strand of a siNA molecule of the invention, for example a siNA molecule having chemical modifications having Formulae I, Formula II Formula III, Formula IV, Formula V, Formula VI, and/or Formula VII.

[0041] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein the chemical modification comprises one or more phosphorothioate internucleotide linkages. For example, in a non-limiting example, the invention features a chemically-modified short interfering nucleic acid (siNA) having about 1, 2, 3, 4, 5, 6, 7, or 8 phosphorothioate internucleotide linkages in one siNA strand. In yet another embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) individually having about 1, 2, 3, 4, 5, 6, 7, or 8 phosphorothioate internucleotide linkages in both siNA strands. The phosphorothioate internucleotide linkages can be present in one or both oligonucleotide strands of the siNA duplex, for example in the sense strand, the antisense strand, or both strands. The siNA molecules of the invention can comprise one or more phosphorothioate internucleotide linkages at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense strand, antisense strand, or both strands. For example, an exemplary siNA molecule of the invention can comprise between about 1 and about 5 phosphorothioate internucleotide linkages at the 5′-end of the sense strand, the antisense strand, or both strands. In another non-limiting example, an exemplary siNA molecule of the invention can comprise one or more pyrimidine phosphorothioate internucleotide linkages in the sense strand, the antisense strand, or both strands. In yet another non-limiting example, an exemplary siNA molecule of the invention can comprise one or more purine phosphorothioate internucleotide linkages in the sense strand, the antisense strand, or both strands.

[0042] In one embodiment, the invention features a siNA molecule, wherein the sense strand comprises one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the sense strand; and wherein the antisense strand comprises any of between 1 and 10, specifically about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the antisense strand. In another embodiment, one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 pyrimidine nucleotides of the sense and/or antisense siNA stand are chemically-modified with 2′-deoxy, 2′-O-methyl and/or 2′-deoxy-2′-fluoro nucleotides, with or without one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 phosphorothioate internucleotide linkages and/or a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends, being present in the same or different strand.

[0043] In another embodiment, the invention features a siNA molecule, wherein the sense strand comprises between 1 and 5, specifically about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the sense strand; and wherein the antisense strand comprises any of between 1 and 5, specifically about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the antisense strand. In another embodiment, one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more, pyrimidine nucleotides of the sense and/or antisense siNA stand are chemically-modified with 2′-deoxy, 2′-O-methyl and/or 2′-deoxy-2′-fluoro nucleotides, with or without between 1 and 5, for example about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages and/or a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends, being present in the same or different strand.

[0044] In one embodiment, the invention features a siNA molecule, wherein the antisense strand comprises one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 phosphorothioate internucleotide linkages, and/or between one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the sense strand; and wherein the antisense strand comprises any of between 1 and 10, specifically about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the antisense strand. In another embodiment, one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 pyrimidine nucleotides of the sense and/or antisense siNA stand are chemically-modified with 2′-deoxy, 2′-O-methyl and/or 2′-deoxy-2′-fluoro nucleotides, with or without one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 phosphorothioate internucleotide linkages and/or a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends, being present in the same or different strand.

[0045] In another embodiment, the invention features a siNA molecule, wherein the antisense strand comprises between 1 and 5, specifically about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the sense strand; and wherein the antisense strand comprises any of between 1 and 5, specifically about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages, and/or one or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, and/or one or more universal base modified nucleotides, and optionally a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends of the antisense strand. In another embodiment, one or more, for example about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 pyrimidine nucleotides of the sense and/or antisense siNA stand are chemically-modified with 2′-deoxy, 2′-O-methyl and/or 2′-deoxy-2′-fluoro nucleotides, with or without between 1 and 5, for example about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages and/or a terminal cap molecule at the 3′ end, the 5′ end, or both of the 3′ and 5′-ends, being present in the same or different strand.

[0046] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule having between about 1 and 5, specifically 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages in each strand of the siNA molecule.

[0047] In another embodiment, the invention features a siNA molecule comprising 2′-5′ internucleotide linkages. The 2′-5′ internucleotide linkage(s) can be at the 5′-end, 3′-end, or both 5′ and 3′ ends of one or both siNA sequence strands. In addition, the 2′-5′ internucleotide linkage(s) can be present at various other positions within one or both siNA sequence strands, for example, every internucleotide linkage of a pyrimidine nucleotide in one or both strands of the siNA molecule can comprise a 2′-5′ internucleotide linkage, or every internucleotide linkage of a purine nucleotide in one or both strands of the siNA molecule can comprise a 2′-5′ internucleotide linkage.

[0048] In another embodiment, a chemically-modified siNA molecule of the invention comprises a duplex having two strands, one or both of which can be chemically-modified, wherein each strand is between about 18 and about 27 nucleotides in length, wherein the duplex has between about 18 and about 23 base pairs, and wherein the chemical modification comprises a structure having any of Formulae I-VII. For example, an exemplary chemically-modified siNA molecule of the invention comprises a duplex having two strands, one or both of which can be chemically-modified with a chemical modification having any of Formulae I-VII, wherein each strand consists of about 21 nucleotides, each having two about 2 nucleotide 3′-terminal nucleotide overhangs, and wherein the duplex has 19 base pairs.

[0049] In another embodiment, a siNA molecule of the invention comprises a single-stranded hairpin structure, wherein the siNA is between about 36 and about 70 nucleotides in length having between about 18 and about 23 base pairs, and wherein the siNA can include a chemical modification comprising a structure having any of Formulae I-VII. For example, an exemplary chemically-modified siNA molecule of the invention comprises a linear oligonucleotide having between 42 and 50 nucleotides that is chemically-modified with a chemical modification having any of Formulae I-VII, wherein the linear oligonucleotide forms a hairpin structure having 19 base pairs and a 2 nucleotide 3′-terminal nucleotide overhang.

[0050] In another embodiment, a linear hairpin siNA molecule of the invention contains a stem loop motif, wherein the loop portion of the siNA molecule is biodegradable. For example, a linear hairpin siNA molecule of the invention is designed such that degradation of the loop portion of the siNA molecule in vivo can generate a double-stranded siNA molecule with 3′-terminal overhangs, such as 3′-terminal nucleotide overhangs comprising about 2 nucleotides.

[0051] In another embodiment, a siNA molecule of the invention comprises a circular nucleic acid molecule, wherein the siNA is between about 38 and about 70 nucleotides in length having between about 18 and about 23 base pairs, and wherein the siNA can include a chemical modification, which comprises a structure having any of Formulae I-VII. For example, an exemplary chemically-modified siNA molecule of the invention comprises a circular oligonucleotide having between 42 and 50 nucleotides that is chemically-modified with a chemical modification having any of Formulae I-VII, wherein the circular oligonucleotide forms a dumbbell-shaped structure having 19 base pairs and 2 loops.

[0052] In another embodiment, a circular siNA molecule of the invention contains two loop motifs, wherein one or both loop portions of the siNA molecule is biodegradable. For example, a circular siNA molecule of the invention is designed such that degradation of the loop portions of the siNA molecule in vivo can generate a double-stranded siNA molecule with 3′-terminal overhangs, such as 3′-terminal nucleotide overhangs comprising about 2 nucleotides.

[0053] In one embodiment, a siNA molecule of the invention comprises one or more abasic residues, for example a compound having Formula V:

[0054] wherein each R3, R4, R5, R6, R7, R8, R10, R11, R12, and R13 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, Cl, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO2, NO2, N3, NH2, aminoalkyl, aminoacid, aminoacyl, ONH2, O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I; R9 is O, S, CH2, S═O, CHF, or CF2.

[0055] In one embodiment, a siNA molecule of the invention comprises one or more inverted abasic residues, for example a compound having Formula VI:

[0056] wherein each R3, R4, R5, R6, R7, R8, R10, R11, R12, and R13 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, Cl, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO2, NO2, N3, NH2, aminoalkyl, aminoacid, aminoacyl, ONH2, O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I; R9 is O, S, CH2, S═O, CHF, or CF2, and either R2, R3, R8 or R13 serve as points of attachment to the siNA molecule of the invention.

[0057] In another embodiment, a siNA molecule of the invention comprises at least one (e.g., about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more) substituted polyalkyl moieties, for example a compound having Formula VII:

[0058] wherein each n is independently an integer from 1 to 12, each R1, R2 and R3 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, Cl, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO2, NO2, N3, NH2, aminoalkyl, aminoacid, aminoacyl, ONH2, O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I, and either R1, R2 or R3 serve as points of attachment to the siNA molecule of the invention.

[0059] In another embodiment, the invention features a compound having Formula VII, wherein R1 and R2 are hydroxyl (OH) groups, n=1, and R3 comprises O and is the point of attachment to the 3′-end, the 5-end, or both 3′ and 5′-ends of one or both strands of a double-stranded siNA molecule of the invention or to a single-stranded siNA molecule of the invention. This modification is referred to herein as “glyceryl” (for example modification 6 in FIG. 19).

[0060] In another embodiment, a moiety having any of Formulae V, VI or VII of the invention is at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of a siNA molecule of the invention. For example, a moiety having Formulae V, VI or VII can be present at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the antisense strand, the sense strand, or both antisense and sense strands of the siNA molecule. In addition, a moiety having Formulae V, VI or VII can be present at the 3′-end or the 5′-end of a hairpin siNA molecule as described herein.

[0061] In another embodiment, a siNA molecule of the invention comprises an abasic residue having Formula V or VI, wherein the abasic residue having Formula V or VI is connected to the siNA construct in a 3′-3′, 3′-2′, 2′-3′, or 5′-5′ configuration, such as at the 3′-end, 5′-end, or both 3′ and ‘5’-ends of one or both siNA strands.

[0062] In one embodiment, a siNA molecule of the invention comprises one or more (e.g., about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more) locked nucleic acid (LNA) nucleotides, for example at the 5′-end, 3′-end, 5′ and 3′-end, or any combination thereof, of the siNA molecule.

[0063] In another embodiment, a siNA molecule of the invention comprises one or more (e.g., about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more) acyclic nucleotides, for example at the 5′-end, 3′-end, 5′ and 3′-end, or any combination thereof, of the siNA molecule.

[0064] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule of the invention capable of mediating RNA interference (RNAi) against EGFR inside a cell or reconstituted in vitro system, wherein the chemically-modified siNA comprises a sense region, where any (e.g., one or more or all) pyrimidine nucleotides present in the sense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and where any (e.g., one or more or all) purine nucleotides present in the sense region are 2′-deoxy purine nucleotides (e.g., wherein all purine nucleotides are 2′-deoxy purine nucleotides or alternately a plurality of purine nucleotides are 2′-deoxy purine nucleotides).

[0065] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule of the invention capable of mediating RNA interference (RNAi) against EGFR inside a cell or reconstituted in vitro system, wherein the chemically-modified siNA comprises a sense region, where any (e.g., one or more or all) pyrimidine nucleotides present in the sense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and where any (e.g., one or more or all) purine nucleotides present in the sense region are 2′-deoxy purine nucleotides (e.g., wherein all purine nucleotides are 2′-deoxy purine nucleotides or alternately a plurality of purine nucleotides are 2′-deoxy purine nucleotides), wherein any nucleotides comprising a 3′-terminal nucleotide overhang that are present in said sense region are 2′-deoxy nucleotides.

[0066] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule of the invention capable of mediating RNA interference (RNAi) against EGFR inside a cell or reconstituted in vitro system, wherein the chemically-modified siNA comprises an antisense region, where any (e.g., one or more or all) pyrimidine nucleotides present in the antisense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and wherein any (e.g., one or more or all) purine nucleotides present in the antisense region are 2′-O-methyl purine nucleotides (e.g., wherein all purine nucleotides are 2′-O-methyl purine nucleotides or alternately a plurality of purine nucleotides are 2′-O-methyl purine nucleotides).

[0067] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule of the invention capable of mediating RNA interference (RNAi) against EGFR inside a cell or reconstituted in vitro system, wherein the chemically-modified siNA comprises a sense region, where one or more pyrimidine nucleotides present in the sense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and where one or more purine nucleotides present in the sense region are 2′-deoxy purine nucleotides (e.g., wherein all purine nucleotides are 2′-deoxy purine nucleotides or alternately a plurality of purine nucleotides are 2′-deoxy purine nucleotides), and inverted deoxy abasic modifications that are optionally present at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense region, the sense region optionally further comprising a 3′-terminal nucleotide overhang having between about 1 and about 4 (e.g, about 1, 2, 3, or 4) 2′-deoxyribonucleotides; and wherein the chemically-modified short interfering nucleic acid molecule comprises an antisense region, where one or more pyrimidine nucleotides present in the antisense region are 2′-deoxy-2′-fluoro substituted pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and wherein one or more purine nucleotides present in the antisense region are 2′-O-methyl purine nucleotides (e.g., wherein all purine nucleotides are 2′-O-methyl purine nucleotides or alternately a plurality of purine nucleotides are 2′-O-methyl purine nucleotides), and a terminal cap modification, such as any modification described herein or shown in FIG. 10, that is optionally present at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the antisense sequence, the antisense region optionally further comprising a 3′-terminal overhang having between about 1 and about 4 (e.g, about 1, 2, 3, or 4) 2′-deoxynucleotides, wherein the overhang nucleotides can further comprise one or more (e.g., 1, 2, 3, or 4 ) phosphorothioate internucleotide linkages. Non-limiting examples of these chemically-modified siNAs are shown in FIGS. 4 and 5 herein.

[0068] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule of the invention capable of mediating RNA interference (RNAi) against EGFR inside a cell or reconstituted in vitro system, wherein the siNA comprises a sense region, where one or more pyrimidine nucleotides present in the sense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and where one or more purine nucleotides present in the sense region are purine ribonucleotides (e.g., wherein all purine nucleotides are purine ribonucleotides or alternately a plurality of purine nucleotides are purine ribonucleotides), and inverted deoxy abasic modifications that are optionally present at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense region, the sense region optionally further comprising a 3′-terminal nucleotide overhang having between about 1 and about 4 (e.g, about 1, 2, 3, or 4) 2′-deoxyribonucleotides; and wherein the siNA comprises an antisense region, where one or more pyrimidine nucleotides present in the antisense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and wherein any purine nucleotides present in the antisense region are 2′-O-methyl purine nucleotides (e.g., wherein all purine nucleotides are 2′-O-methyl purine nucleotides or alternately a plurality of purine nucleotides are 2′-O-methyl purine nucleotides), and a terminal cap modification, such as any modification described herein or shown in FIG. 10, that is optionally present at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the antisense sequence, the antisense region optionally further comprising a 3′-terminal nucleotide overhang having between about 1 and about 4 (e.g, about 1, 2, 3, or 4) 2′-deoxynucleotides, wherein the overhang nucleotides can further comprise one or more (e.g., 1, 2, 3, or 4 ) phosphorothioate internucleotide linkages. Non-limiting examples of these chemically-modified siNAs are shown in FIGS. 4 and 5 herein.

[0069] In one embodiment, the invention features a chemically-modified short interfering nucleic acid (siNA) molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein the chemical modification comprises a conjugate covalently attached to the siNA molecule. In another embodiment, the conjugate is covalently attached to the siNA molecule via a biodegradable linker. In one embodiment, the conjugate molecule is attached at the 3′-end of either the sense strand, the antisense strand, or both strands of the siNA. In another embodiment, the conjugate molecule is attached at the 5′-end of either the sense strand, the antisense strand, or both strands of the siNA. In yet another embodiment, the conjugate molecule is attached to both the 3′-end and the 5′-end of either the sense strand, the antisense strand, or both strands of the siNA, or any combination thereof. In one embodiment, a conjugate molecule of the invention comprises a molecule that facilitates delivery of a siNA molecule into a biological system such as a cell. In another embodiment, the conjugate molecule attached to the siNA is a poly ethylene glycol, human serum albumin, or a ligand for a cellular receptor that can mediate cellular uptake. Examples of specific conjugate molecules contemplated by the instant invention that can be attached to siNA molecules are described in Vargeese et al., U.S. Ser. No. 60/311,865, incorporated by reference herein.

[0070] In one embodiment, the invention features a siNA molecule capable of mediating RNA interference (RNAi) against EGFR inside a cell, wherein one or both strands of the siNA comprise ribonucleotides at positions within the siNA that are critical for siNA mediated RNAi in a cell. All other positions within the siNA can include chemically-modified nucleotides and/or non-nucleotides such as nucleotides and or non-nucleotides having any of Formulae I, II, III, IV, V, VI, or VII or any combination thereof to the extent that the ability of the siNA molecule to support RNAi activity in a cell is maintained.

[0071] In one embodiment, the invention features a method for modulating the expression of a EGFR gene within a cell, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR gene; and (b) introducing the siNA molecule into a cell under conditions suitable to modulate the expression of the EGFR gene in the cell.

[0072] In one embodiment, the invention features a method for modulating the expression of a EGFR gene within a cell, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR gene and wherein the sense strand sequence of the siNA is identical to the complementary sequence of the EGFR RNA; and (b) introducing the siNA molecule into a cell under conditions suitable to modulate the expression of the EGFR gene in the cell.

[0073] In another embodiment, the invention features a method for modulating the expression of more than one EGFR gene within a cell, comprising: (a) synthesizing siNA molecules of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR genes; and (b) introducing the siNA molecules into a cell under conditions suitable to modulate the expression of the EGFR genes in the cell.

[0074] In another embodiment, the invention features a method for modulating the expression of more than one EGFR gene within a cell, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR gene and wherein the sense strand sequence of the siNA is identical to the complementary sequence of the EGFR RNA; and (b) introducing the siNA molecules into a cell under conditions suitable to modulate the expression of the EGFR genes in the cell.

[0075] In one embodiment, the invention features a method of modulating the expression of a EGFR gene in a tissue explant, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR gene; (b) introducing the siNA molecule into a cell of the tissue explant derived from a particular organism under conditions suitable to modulate the expression of the EGFR gene in the tissue explant, and (c) optionally introducing the tissue explant back into the organism the tissue was derived from or into another organism under conditions suitable to modulate the expression of the EGFR gene in that organism.

[0076] In one embodiment, the invention features a method of modulating the expression of a EGFR gene in a tissue explant, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR gene and wherein the sense strand sequence of the siNA is identical to the complementary sequence of the EGFR RNA; (b) introducing the siNA molecule into a cell of the tissue explant derived from a particular organism under conditions suitable to modulate the expression of the EGFR gene in the tissue explant, and (c) optionally introducing the tissue explant back into the organism the tissue was derived from or into another organism under conditions suitable to modulate the expression of the EGFR gene in that organism.

[0077] In another embodiment, the invention features a method of modulating the expression of more than one EGFR gene in a tissue explant, comprising: (a) synthesizing siNA molecules of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR genes; (b) introducing the siNA molecules into a cell of the tissue explant derived from a particular organism under conditions suitable to modulate the expression of the EGFR genes in the tissue explant, and (c) optionally introducing the tissue explant back into the organism the tissue was derived from or into another organism under conditions suitable to modulate the expression of the EGFR genes in that organism.

[0078] In one embodiment, the invention features a method of modulating the expression of a EGFR gene in an organism, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR gene; and (b) introducing the siNA molecule into the organism under conditions suitable to modulate the expression of the EGFR gene in the organism.

[0079] In another embodiment, the invention features a method of modulating the expression of more than one EGFR gene in an organism, comprising: (a) synthesizing siNA molecules of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of the EGFR genes; and (b) introducing the siNA molecules into the organism under conditions suitable to modulate the expression of the EGFR genes in the organism.

[0080] The siNA molecules of the invention can be designed to inhibit EGFR gene expression through RNAi targeting of a variety of RNA molecules. In one embodiment, the siNA molecules of the invention are used to target various RNAs corresponding to a target gene. Non-limiting examples of such RNAs include messenger RNA (mRNA), alternate RNA splice variants of target gene(s), post-transcriptionally modified RNA of target gene(s), pre-mRNA of target gene(s). If alternate splicing produces a family of transcipts that are distinguished by usage of appropriate exons, the instant invention can be used to inhibit gene expression through the appropriate exons to specifically inhibit or to distinguish among the functions of gene family members. For example, a protein that contains an alternatively spliced transmembrane domain can be expressed in both membrane bound and secreted forms. Use of the invention to target the exon containing the transmembrane domain can be used to determine the functional consequences of pharmaceutical targeting of membrane bound as opposed to the secreted form of the protein. Non-limiting examples of applications of the invention relating to targeting these RNA molecules include therapeutic pharmaceutical applications, pharmaceutical discovery applications, molecular diagnostic and gene function applications, and gene mapping, for example using single nucleotide polymorphism mapping with siNA molecules of the invention. Such applications can be implemented using known gene sequences or from partial sequences available from an expressed sequence tag (EST).

[0081] In another embodiment, the siNA molecules of the invention are used to target conserved sequences corresponding to a gene family or gene families such as EGFR genes HER1, HER2, HER3, and/or HER4. As such, siNA molecules targeting multiple EGFR targets can provide increased therapeutic effect. In addition, siNA can be used to characterize pathways of gene function in a variety of applications. For example, the present invention can be used to inhibit the activity of target gene(s) in a pathway to determine the function of uncharacterized gene(s) in gene function analysis, mRNA function analysis, or translational analysis. The invention can be used to determine potential target gene pathways involved in various diseases and conditions toward pharmaceutical development. The invention can be used to understand pathways of gene expression involved in development, such as prenatal development, postnatal development and/or aging.

[0082] In one embodiment, siNA molecule(s) and/or methods of the invention are used to inhibit the expression of gene(s) that encode RNA referred to by Genbank Accession, for example EGFR genes such as HER1 (for example Genbank Accession No. NM_(—)005228), HER2 (for example Genbank Accession No. NM_(—)004448), HER3 (for example Genbank Accession No. NM_(—)001982), and HER4 (for example Genbank Accession No. NM_(—)005235). In another embodiment, siNA molecule(s) and/or methods of the invention are used to target RNA sequence(s) referred to by Genbank Accession number, for example EGFR genes such as HER1 (for example Genbank Accession No. NM_(—)005228), HER2 (for example Genbank Accession No. NM_(—)004448), HER3 (for example Genbank Accession No. NM_(—)001982), and HER4 (for example Genbank Accession No. NM_(—)005235). Such sequences are readily obtained using these Genbank Accession numbers.

[0083] In one embodiment, the invention features a method comprising: (a) generating a randomized library of siNA constructs having a predetermined complexity, such as of 4^(N), where N represents the number of base paired nucleotides in each of the siNA construct strands (eg. for a siNA construct having 21-nucleotide sense and antisense strands with 19 base pairs, the complexity would be 4¹⁹); and (b) assaying the siNA constructs of (a) above, under conditions suitable to determine RNAi target sites within the target HER2 RNA sequence. In another embodiment, the siNA molecules of (a) have strands of a fixed length, for example about 23 nucleotides in length. In yet another embodiment, the siNA molecules of (a) are of differing length, for example having strands of about 19 to about 25 (e.g., about 19, 20, 21, 22, 23, 24, or 25) nucleotides in length. In yet another embodiment, the assay can comprise a reconstituted in vitro siNA assay as described in Example 8 herein. In another embodiment, the assay can comprise a cell culture system in which target RNA is expressed. In another embodiment, fragments of HER2 RNA are analyzed for detectable levels of cleavage, for example by gel electrophoresis, northern blot analysis, or RNAse protection assays, to determine the most suitable target site(s) within the target HER2 RNA sequence. In another embodiment, the target HER2 RNA sequence can be obtained as is known in the art, for example, by cloning and/or transcription for in vitro systems, and by cellular expression in in vivo systems.

[0084] In another embodiment, the invention features a method comprising: (a) analyzing the sequence of a RNA target encoded by an HER2 gene; (b) synthesizing one or more sets of siNA molecules having sequence complementary to one or more regions of the RNA of (a); and (c) assaying the siNA molecules of (b) under conditions suitable to determine RNAi targets within the target RNA sequence. In another embodiment, the siNA molecules of (b) have strands of a fixed length, for example about 23 nucleotides in length. In yet another embodiment, the siNA molecules of (b) are of differing length, for example having strands of about 19 to about 25 (e.g., about 19, 20, 21, 22, 23, 24, or 25) nucleotides in length. In yet another embodiment, the assay can comprise a reconstituted in vitro siNA assay as described in Example 8 herein. In another embodiment, the assay can comprise a cell culture system in which target RNA is expressed. Fragments of HER2 RNA are analyzed for detectable levels of cleavage, for example by gel electrophoresis, northern blot analysis, or RNAse protection assays, to determine the most suitable target site(s) within the target HER2 RNA sequence. The target HER2 RNA sequence can be obtained as is known in the art, for example, by cloning and/or transcription for in vitro systems, and by expression in in vivo systems.

[0085] By “target site” is meant a sequence within a target RNA that is “targeted” for cleavage mediated by a siNA construct which contains sequences within its antisense region that are complementary to the target sequence.

[0086] By “detectable level of cleavage” is meant cleavage of target RNA (and formation of cleaved product RNAs) to an extent sufficient to discern cleavage products above the background of RNAs produced by random degradation of the target RNA. Production of cleavage products from 1-5% of the target RNA is sufficient to detect above the background for most methods of detection.

[0087] In one embodiment, the invention features a composition comprising a siNA molecule of the invention, which can be chemically-modified, in a pharmaceutically acceptable carrier or diluent. In another embodiment, the invention features a pharmaceutical composition comprising siNA molecules of the invention, which can be chemically-modified, targeting one or more genes in a pharmaceutically acceptable carrier or diluent. In another embodiment, the invention features a method for treating or preventing a disease or condition in a patient, comprising administering to the patient a composition of the invention under conditions suitable for the treatment or prevention of the disease or condition in the patient, alone or in conjunction with one or more other therapeutic compounds. In yet another embodiment, the invention features a method for reducing or preventing tissue rejection in a patient comprising administering to the patient a composition of the invention under conditions suitable for the reduction or prevention of tissue rejection in the patient.

[0088] In another embodiment, the invention features a method for validating a EGFR gene target, comprising: (a) synthesizing a siNA molecule of the invention, which can be chemically-modified, wherein one of the siNA strands includes a sequence complementary to RNA of a EGFR target gene; (b) introducing the siNA molecule into a cell, tissue, or organism under conditions suitable for modulating expression of the EGFR target gene in the cell, tissue, or organism; and (c) determining the function of the gene by assaying for any phenotypic change in the cell, tissue, or organism.

[0089] In one embodiment, the invention features a kit containing a siNA molecule of the invention, which can be chemically-modified, that can be used to modulate the expression of a EGFR target gene in a cell, tissue, or organism. In another embodiment, the invention features a kit containing more than one siNA molecule of the invention, which can be chemically-modified, that can be used to modulate the expression of more than one EGFR target gene in a cell, tissue, or organism.

[0090] In one embodiment, the invention features a cell containing one or more siNA molecules of the invention, which can be chemically-modified. In another embodiment, the cell containing a siNA molecule of the invention is a mammalian cell. In yet another embodiment, the cell containing a siNA molecule of the invention is a human cell.

[0091] In one embodiment, the synthesis of a siNA molecule of the invention, which can be chemically-modified, comprises: (a) synthesis of two complementary strands of the siNA molecule; (b) annealing the two complementary strands together under conditions suitable to obtain a double-stranded siNA molecule. In another embodiment, synthesis of the two complementary strands of the siNA molecule is by solid phase oligonucleotide synthesis. In yet another embodiment, synthesis of the two complementary strands of the siNA molecule is by solid phase tandem oligonucleotide synthesis.

[0092] In one embodiment, the invention features a method for synthesizing a siNA duplex molecule comprising: (a) synthesizing a first oligonucleotide sequence strand of the siNA molecule, wherein the first oligonucleotide sequence strand comprises a cleavable linker molecule that can be used as a scaffold for the synthesis of the second oligonucleotide sequence strand of the siNA; (b) synthesizing the second oligonucleotide sequence strand of siNA on the scaffold of the first oligonucleotide sequence strand, wherein the second oligonucleotide sequence strand further comprises a chemical moiety than can be used to purify the siNA duplex; (c) cleaving the linker molecule of (a) under conditions suitable for the two siNA oligonucleotide strands to hybridize and form a stable duplex; and (d) purifying the siNA duplex utilizing the chemical moiety of the second oligonucleotide sequence strand. In another embodiment, cleavage of the linker molecule in (c) above takes place during deprotection of the oligonucleotide, for example under hydrolysis conditions using an alkylamine base such as methylamine. In another embodiment, the method of synthesis comprises solid phase synthesis on a solid support such as controlled pore glass (CPG) or polystyrene, wherein the first sequence of (a) is synthesized on a cleavable linker, such as a succinyl linker, using the solid support as a scaffold. The cleavable linker in (a) used as a scaffold for synthesizing the second strand can comprise similar reactivity as the solid support derivatized linker, such that cleavage of the solid support derivatized linker and the cleavable linker of (a) takes place concomitantly. In another embodiment, the chemical moiety of (b) that can used to isolate the attached oligonucleotide sequence comprises a trityl group, for example a dimethoxytrityl group, which can be employed in a trityl-on synthesis strategy as described herein. In yet another embodiment, the chemical moiety, such as a dimethoxytrityl group, is removed during purification, for example using acidic conditions.

[0093] In a further embodiment, the method for siNA synthesis is a solution phase synthesis or hybrid phase synthesis wherein both strands of the siNA duplex are synthesized in tandem using a cleavable linker attached to the first sequence which acts a scaffold for synthesis of the second sequence. Cleavage of the linker under conditions suitable for hybridization of the separate siNA sequence strands results in formation of the double-stranded siNA molecule.

[0094] In another embodiment, the invention features a method for synthesizing a siNA duplex molecule comprising: (a) synthesizing one oligonucleotide sequence strand of the siNA molecule, wherein the sequence comprises a cleavable linker molecule that can be used as a scaffold for the synthesis of another oligonucleotide sequence; (b) synthesizing a second oligonucleotide sequence having complementarity to the first sequence strand on the scaffold of (a), wherein the second sequence comprises the other strand of the double-stranded siNA molecule and wherein the second sequence further comprises a chemical moiety than can be used to isolate the attached oligonucleotide sequence; (c) purifying the product of (b) utilizing the chemical moiety of the second oligonucleotide sequence strand under conditions suitable for isolating the full-length sequence comprising both siNA oligonucleotide strands connected by the cleavable linker; and (d) under conditions suitable for the two siNA oligonucleotide strands to hybridize and form a stable duplex. In another embodiment, cleavage of the linker molecule in (c) above takes place during deprotection of the oligonucleotide, for example under hydrolysis conditions. In another embodiment, cleavage of the linker molecule in (c) above takes place after deprotection of the oligonucleotide. In another embodiment, the method of synthesis comprises solid phase synthesis on a solid support such as controlled pore glass (CPG) or polystyrene, wherein the first sequence of (a) is synthesized on a cleavable linker, such as a succinyl linker, using the solid support as a scaffold. The cleavable linker in (a) used as a scaffold for synthesizing the second strand can comprise similar reactivity or differing reactivity as the solid support derivatized linker, such that cleavage of the solid support derivatized linker and the cleavable linker of (a) takes place either concomitantly or sequentially. In another embodiment, the chemical moiety of (b) that can used to isolate the attached oligonucleotide sequence comprises a trityl group, for example a dimethoxytrityl group.

[0095] In another embodiment, the invention features a method for making a double-stranded siNA molecule in a single synthetic process, comprising: (a) synthesizing an oligonucleotide having a first and a second sequence, wherein the first sequence is complementary to the second sequence, and the first oligonucleotide sequence is linked to the second sequence via a cleavable linker, and wherein a terminal 5′-protecting group, for example a 5′-O-dimethoxytrityl group (5′-O-DMT) remains on the oligonucleotide having the second sequence; (b) deprotecting the oligonucleotide whereby the deprotection results in the cleavage of the linker joining the two oligonucleotide sequences; and (c) purifying the product of (b) under conditions suitable for isolating the double-stranded siNA molecule, for example using a trityl-on synthesis strategy as described herein.

[0096] In one embodiment, the invention features siNA constructs that mediate RNAi against EGFR, wherein the siNA construct comprises one or more chemical modifications, for example one or more chemical modifications having Formulae I, II, III, IV, V, VI or VII that increases the nuclease resistance of the siNA construct.

[0097] In another embodiment, the invention features a method for generating siNA molecules with increased nuclease resistance comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having increased nuclease resistance.

[0098] In one embodiment, the invention features siNA constructs that mediate RNAi against EGFR, wherein the siNA construct comprises one or more chemical modifications described herein that modulates the binding affinity between the sense and antisense strands of the siNA construct.

[0099] In another embodiment, the invention features a method for generating siNA molecules with increased binding affinity between the sense and antisense strands of the siNA molecule comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having increased binding affinity between the sense and antisense strands of the siNA molecule.

[0100] In one embodiment, the invention features siNA constructs that mediate RNAi against EGFR, wherein the siNA construct comprises one or more chemical modifications described herein that modulates the binding affinity between the antisense strand of the siNA construct and a complementary target RNA sequence within a cell.

[0101] In another embodiment, the invention features a method for generating siNA molecules with increased binding affinity between the antisense strand of the siNA molecule and a complementary target RNA sequence, comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having increased binding affinity between the antisense strand of the siNA molecule and a complementary target RNA sequence.

[0102] In one embodiment, the invention features siNA constructs that mediate RNAi against EGFR, wherein the siNA construct comprises one or more chemical modifications described herein that modulate the polymerase activity of a cellular polymerase capable of generating additional endogenous siNA molecules having sequence homology to the chemically-modified siNA construct.

[0103] In another embodiment, the invention features a method for generating siNA molecules capable of mediating increased polymerase activity of a cellular polymerase capable of generating additional endogenous siNA molecules having sequence homology to the chemically-modified siNA molecule comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules capable of mediating increased polymerase activity of a cellular polymerase capable of generating additional endogenous siNA molecules having sequence homology to the chemically-modified siNA molecule.

[0104] In one embodiment, the invention features chemically-modified siNA constructs that mediate RNAi against EGFR in a cell, wherein the chemical modifications do not significantly effect the interaction of siNA with a target RNA molecule and/or proteins or other factors that are essential for RNAi in a manner that would decrease the efficacy of RNAi mediated by such siNA constructs.

[0105] In another embodiment, the invention features a method for generating siNA molecules with improved RNAi activity against EGFR, comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having improved RNAi activity.

[0106] In yet another embodiment, the invention features a method for generating siNA molecules with improved RNAi activity against an EGFR target RNA, comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having improved RNAi activity against the target RNA.

[0107] In one embodiment, the invention features siNA constructs that mediate RNAi against EGFR, wherein the siNA construct comprises one or more chemical modifications described herein that modulates the cellular uptake of the siNA construct.

[0108] In another embodiment, the invention features a method for generating siNA molecules against EGFR with improved cellular uptake, comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having improved cellular uptake.

[0109] In one embodiment, the invention features siNA constructs that mediate RNAi against EGFR, wherein the siNA construct comprises one or more chemical modifications described herein that increases the bioavailability of the siNA construct, for example by attaching polymeric conjugates such as polyethyleneglycol or equivalent conjugates that improve the pharmacokinetics of the siNA construct, or by attaching conjugates that target specific tissue types or cell types in vivo. Non-limiting examples of such conjugates are described in Vargeese et al., U.S. Ser. No. 60/311,865 incorporated by reference herein.

[0110] In one embodiment, the invention features a method for generating siNA molecules of the invention with improved bioavailability, comprising (a) introducing a conjugate into the structure of a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having improved bioavailability. Such conjugates can include ligands for cellular receptors such as peptides derived from naturally occurring protein ligands, protein localization sequences including cellular ZIP code sequences, antibodies, nucleic acid aptamers, vitamins and other co-factors such as folate and N-acetylgalactosamine, polymers such as polyethyleneglycol (PEG), phospholipids, polyamines such as spermine or spermidine, and others.

[0111] In another embodiment, the invention features a method for generating siNA molecules of the invention with improved bioavailability, comprising (a) introducing an excipient formulation to a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having improved bioavailability. Such excipients include polymers such as cyclodextrines, lipids, cationic lipids, polyamines, phospholipids, and others.

[0112] In another embodiment, the invention features a method for generating siNA molecules of the invention with improved bioavailability, comprising (a) introducing nucleotides having any of Formulae I-VII into a siNA molecule, and (b) assaying the siNA molecule of step (a) under conditions suitable for isolating siNA molecules having improved bioavailability.

[0113] In another embodiment, polyethylene glycol (PEG) can be covalently attached to siNA compounds of the present invention. The attached PEG can be any molecular weight, preferably from about 2,000 to about 50,000 daltons (Da).

[0114] The present invention can be used alone or as a component of a kit having at least one of the reagents necessary to carry out the in vitro or in vivo introduction of RNA to test samples and/or subjects. For example, preferred components of the kit include the siNA and a vehicle that promotes introduction of the siNA. Such a kit can also include instructions to allow a user of the kit to practice the invention.

[0115] The term “short interfering nucleic acid”, “siNA”, “short interfering RNA”, “siRNA”, “short interfering nucleic acid molecule”, “short interfering oligonucleotide molecule”, or “chemically-modified short interfering nucleic acid moleule” as used herein refers to any nucleic acid molecule capable of mediating RNA interference “RNAi” or gene silencing; see for example Bass, 2001, Nature, 411, 428-429; Elbashir et al., 2001, Nature, 411, 494-498; and Kreutzer et al., International PCT Publication No. WO 00/44895; Zemicka-Goetz et al., International PCT Publication No. WO 01/36646; Fire, International PCT Publication No. WO 99/32619; Plaetinck et al., International PCT Publication No. WO 00/01846; Mello and Fire, International PCT Publication No. WO 01/29058; Deschamps-Depaillette, International PCT Publication No. WO 99/07409; and Li et al., International PCT Publication No. WO 00/44914. Non limiting examples of siRNA molecules of the invention are shown in FIG. 10. For example the siNA can be a double-stranded polynucleotide molecule comprising self-complementary sense and antisense regions, wherein the antisense region comprises complementarity to a target nucleic acid molecule. The siNA can be a single-stranded hairpin polynucleotide having self-complementary sense and antisense regions, wherein the antisense region comprises complementarity to a target nucleic acid molecule. The siNA can be a circular single-stranded polynucleotide having two or more loop structures and a stem comprising self-complementary sense and antisense regions, wherein the antisense region comprises complementarity to a target nucleic acid molecule, and wherein the circular polynucleotide can be processed either in vivo or in vitro to generate an active siNA capable of mediating RNAi. As used herein, siNA molecules need not be limited to those molecules containing only RNA, but further encompasses chemically-modified nucleotides and non-nucleotides. In certain embodiments, the short interfering nucleic acid molecules of the invention lack 2′-hydroxy (2′-OH) containing nucleotides. Applicant describes in certain embodiments short interfering nucleic acids that do not require the presence of nucleotides having a 2′-hydroxy group for mediating RNAi and as such, short interfering nucleic acid molecules of the invention optionally do not contain any ribonucleotides (e.g., nucleotides having a 2′-OH group). The modified short interfering nucleic acid molecules of the invention can also be referred to as short interfering modified oligonucleotides ““siMON.” As used herein, the term siNA is meant to be equivalent to other terms used to describe nucleic acid molecules that are capable of mediating sequence specific RNAi, for example short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA, short hairpin RNA (shRNA), short interfering oligonucleotide, short interfering nucleic acid, short interfering modified oligonucleotide, chemically-modified siRNA, post-transcriptional gene silencing RNA (ptgsRNA), and others. In addition, as used herein, the term RNAi is meant to be equivalent to other terms used to describe sequence specific RNA interference, such as post transcriptional gene silencing.

[0116] By “modulate” is meant that the expression of the gene, or level of RNA molecule or equivalent RNA molecules encoding one or more proteins or protein subunits, or activity of one or more proteins or protein subunits is up regulated or down regulated, such that expression, level, or activity is greater than or less than that observed in the absence of the modulator. For example, the term “modulate” can mean “inhibit,” but the use of the word “modulate” is not limited to this definition.

[0117] By “inhibit” it is meant that the activity of a gene expression product or level of RNAs or equivalent RNAs encoding one or more gene products is reduced below that observed in the absence of the nucleic acid molecule of the invention. In one embodiment, inhibition with a siNA molecule preferably is below that level observed in the presence of an inactive or attenuated molecule that is unable to mediate an RNAi response. In another embodiment, inhibition of gene expression with the siNA molecule of the instant invention is greater in the presence of the siNA molecule than in its absence.

[0118] By “gene” or “target gene” is meant, a nucleic acid that encodes an RNA, for example, nucleic acid sequences including, but not limited to, structural genes encoding a polypeptide. The target gene can be a gene derived from a cell, an endogenous gene, a transgene, or exogenous genes such as genes of a pathogen, for example a virus, which is present in the cell after infection thereof. The cell containing the target gene can be derived from or contained in any organism, for example a plant, animal, protozoan, virus, bacterium, or fungus. Non-limiting examples of plants include monocots, dicots, or gymnosperms. Non-limiting examples of animals include vertebrates or invertebrates. Non-limiting examples of fungi include molds or yeasts.

[0119] By “EGFR” as used herein is meant, any epidermal growth factor receptor, such as HER1 (for example encoded by Genbank Accession No. NM_(—)005228), HER2 (for example encoded by Genbank Accession No. NM_(—)004448), HER3 (for example encoded by Genbank Accession No. NM_(—)001982), and HER4 (for example encoded by Genbank Accession No. NM_(—)005235).

[0120] By “EGFR proteins” is meant, protein receptor or a mutant protein derivative thereof, having epidermal growth factor receptor activity, for example, having the ability to bind an epidermal growth factor and/or having tyrosine kinase activity.

[0121] By “highly conserved sequence region” is meant, a nucleotide sequence of one or more regions in a target gene does not vary significantly from one generation to the other or from one biological system to the other.

[0122] By “complementarity” is meant that a nucleic acid can form hydrogen bond(s) with another nucleic acid sequence by either traditional Watson-Crick or other non-traditional types. In reference to the nucleic molecules of the present invention, the binding free energy for a nucleic acid molecule with its complementary sequence is sufficient to allow the relevant function of the nucleic acid to proceed, e.g., RNAi activity. Determination of binding free energies for nucleic acid molecules is well known in the art (see, e.g., Turner et al., 1987, CSH Symp. Quant. Biol. LII pp.123-133; Frier et al., 1986, Proc. Nat. Acad. Sci. USA 83:9373-9377; Turner et al., 1987, J Am. Chem. Soc. 109:3783-3785). A percent complementarity indicates the percentage of contiguous residues in a nucleic acid molecule that can form hydrogen bonds (e.g., Watson-Crick base pairing) with a second nucleic acid sequence (e.g., 5, 6, 7, 8, 9, 10 out of 10 being 50%, 60%, 70%, 80%, 90%, and 100% complementary). “Perfectly complementary” means that all the contiguous residues of a nucleic acid sequence will hydrogen bond with the same number of contiguous residues in a second nucleic acid sequence.

[0123] The siNA molecules of the invention represent a novel therapeutic approach to treat a variety of pathologic indications, such as cancer, including but not limited to breast, lung, prostate, colorectal, brain, esophageal, bladder, pancreatic, cervical, head and neck, and ovarian cancer, melanoma, lymphoma, glioma, multidrug resistant cancers, and any other diseases or conditions that are related to or will respond to the levels of EGFR in a cell or tissue, alone or in combination with other therapies. The reduction of EGFR expression (specifically EGFR gene RNA levels) and thus reduction in the level of the respective protein relieves, to some extent, the symptoms of the disease or condition.

[0124] In one embodiment of the present invention, each sequence of a siNA molecule of the invention is independently 18 to 24 nucleotides in length, in specific embodiments about 18, 19, 20, 21, 22, 23, or 24 nucleotides in length. In another embodiment, the siNA duplexes of the invention independently comprise between about 17 and about 23 base pairs. In yet another embodiment, siNA molecules of the invention comprising hairpin or circular structures are about 35 to about 55 nucleotides in length, or about 38 to about 44 nucleotides in length and comprising 16-22 base pairs. Exemplary siNA molecules of the invention are shown in Tables I-V. Exemplary synthetic siNA molecules of the invention are shown in Table II, III and V and/or FIGS. 12-14.

[0125] As used herein “cell” is used in its usual biological sense, and does not refer to an entire multicellular organism, e.g., specifically does not refer to a human. The cell can be present in an organism, e.g., birds, plants and mammals such as humans, cows, sheep, apes, monkeys, swine, dogs, and cats. The cell can be prokaryotic (e.g., bacterial cell) or eukaryotic (e.g., mammalian or plant cell). The cell can be of somatic or germ line origin, totipotent or pluripotent, dividing or non-dividing. The cell can also be derived from or can comprise a gamete or embryo, a stem cell, or a fully differentiated cell.

[0126] The siNA molecules of the invention are added directly, or can be complexed with cationic lipids, packaged within liposomes, or otherwise delivered to target cells or tissues. The nucleic acid or nucleic acid complexes can be locally administered to relevant tissues ex vivo, or in vivo through injection, infusion pump or stent, with or without their incorporation in biopolymers. In particular embodiments, the nucleic acid molecules of the invention comprise sequences shown in Tables I-V and/or FIGS. 12-14. Examples of such nucleic acid molecules consist essentially of sequences defined in these tables and figures.

[0127] In another aspect, the invention provides mammalian cells containing one or more siNA molecules of this invention. The one or more siNA molecules can independently be targeted to the same or different sites.

[0128] By “RNA” is meant a molecule comprising at least one ribonucleotide residue. By “ribonucleotide” is meant a nucleotide with a hydroxyl group at the 2′ position of a β-D-ribo-furanose moiety. The terms include double-stranded RNA, single-stranded RNA, isolated RNA such as partially purified RNA, essentially pure RNA, synthetic RNA, recombinantly produced RNA, as well as altered RNA that differs from naturally occurring RNA by the addition, deletion, substitution and/or alteration of one or more nucleotides. Such alterations can include addition of non-nucleotide material, such as to the end(s) of the siNA or internally, for example at one or more nucleotides of the RNA. Nucleotides in the RNA molecules of the instant invention can also comprise non-standard nucleotides, such as non-naturally occurring nucleotides or chemically synthesized nucleotides or deoxynucleotides. These altered RNAs can be referred to as analogs or analogs of naturally-occurring RNA.

[0129] By “patient” is meant an organism, which is a donor or recipient of explanted cells or the cells themselves. “Patient” also refers to an organism to which the nucleic acid molecules of the invention can be administered. In one embodiment, a patient is a mammal or mammalian cells. In another embodiment, a patient is a human or human cells.

[0130] The term “phosphorothioate” as used herein refers to an internucleotide linkage having Formula I, wherein Z and/or W comprise a sulfur atom. Hence, the term phosphorothioate refers to both phosphorothioate and phosphorodithioate internucleotide linkages.

[0131] The term “universal base” as used herein refers to nucleotide base analogs that form base pairs with each of the natural DNA/RNA bases with little discrimination between them. Non-limiting examples of universal bases include C-phenyl, C-naphthyl and other aromatic derivatives, inosine, azole carboxamides, and nitroazole derivatives such as 3-nitropyrrole, 4-nitroindole, 5-nitroindole, and 6-nitroindole as known in the art (see for example Loakes, 2001, Nucleic Acids Research, 29, 2437-2447).

[0132] The term “acyclic nucleotide” as used herein refers to any nucleotide having an acyclic ribose sugar, for example where any of the ribose carbons (C1, C2, C3, C4, or C5), are independently or in combination absent from the nucleotide.

[0133] The nucleic acid molecules of the instant invention, individually, or in combination or in conjunction with other drugs, can be used to treat diseases or conditions discussed herein. For example, to treat a particular disease or condition, the siNA molecules can be administered to a patient or can be administered to other appropriate cells evident to those skilled in the art, individually or in combination with one or more drugs under conditions suitable for the treatment.

[0134] In a further embodiment, the siNA molecules can be used in combination with other known treatments to treat conditions or diseases discussed above. For example, the described molecules could be used in combination with one or more known therapeutic agents to treat a disease or condition. Non-limiting examples of other therapeutic agents that can be readily combined with a siNA molecule of the invention are enzymatic nucleic acid molecules, allosteric nucleic acid molecules, antisense, decoy, or aptamer nucleic acid molecules, antibodies such as monoclonal antibodies, small molecules, and other organic and/or inorganic compounds including metals, salts and ions.

[0135] In one embodiment, the invention features an expression vector comprising a nucleic acid sequence encoding at least one siNA molecule of the invention, in a manner that allows expression of the siNA molecule. For example, the vector can contain sequence(s) encoding both strands of a siNA molecule comprising a duplex. The vector can also contain sequence(s) encoding a single nucleic acid molecule that is self complementary and thus forms a siNA molecule. Non-limiting examples of such expression vectors are described in Paul et al., 2002, Nature Biotechnology, 19, 505; Miyagishi and Taira, 2002, Nature Biotechnology, 19, 497; Lee et al., 2002, Nature Biotechnology, 19, 500; and Novina et al., 2002, Nature Medicine, advance online publication doi: 10.1038/mn725.

[0136] In another embodiment, the invention features a mammalian cell, for example, a human cell, including an expression vector of the invention.

[0137] In yet another embodiment, the expression vector of the invention comprises a sequence for a siNA molecule having complementarity to a RNA molecule referred to by a Genbank Accession numbers, for example Genbank Accession No. NM_(—)005228 (HER1), Genbank Accession No. NM_(—)004448 (HER2), Genbank Accession No. NM_(—)001982 (HER3), and Genbank Accession No. NM_(—)005235 (HER4).

[0138] In one embodiment, an expression vector of the invention comprises a nucleic acid sequence encoding two or more siNA molecules, which can be the same or different.

[0139] In another aspect of the invention, siNA molecules that interact with target RNA molecules and down-regulate gene encoding target RNA molecules (for example target RNA molecules referred to by Genbank Accession numbers herein) are expressed from transcription units inserted into DNA or RNA vectors. The recombinant vectors can be DNA plasmids or viral vectors. siNA expressing viral vectors can be constructed based on, but not limited to, adeno-associated virus, retrovirus, adenovirus, or alphavirus. The recombinant vectors capable of expressing the siNA molecules can be delivered as described herein, and persist in target cells. Alternatively, viral vectors can be used that provide for transient expression of siNA molecules. Such vectors can be repeatedly administered as necessary. Once expressed, the siNA molecules bind and down-regulate gene function or expression via RNA interference (RNAi). Delivery of siNA expressing vectors can be systemic, such as by intravenous or intramuscular administration, by administration to target cells ex-planted from a patient followed by reintroduction into the patient, or by any other means that would allow for introduction into the desired target cell.

[0140] By “vectors” is meant any nucleic acid- and/or viral-based technique used to deliver a desired nucleic acid.

[0141] By “comprising” is meant including, but not limited to, whatever follows the word “comprising”. Thus, use of the term “comprising” indicates that the listed elements are required or mandatory, but that other elements are optional and may or may not be present. By “consisting of” is meant including, and limited to, whatever follows the phrase “consisting of”. Thus, the phrase “consisting of” indicates that the listed elements are required or mandatory, and that no other elements may be present. By “consisting essentially of” is meant including any elements listed after the phrase, and limited to other elements that do not interfere with or contribute to the activity or action specified in the disclosure for the listed elements. Thus, the phrase “consisting essentially of” indicates that the listed elements are required or mandatory, but that other elements are optional and may or may not be present depending upon whether or not they affect the activity or action of the listed elements.

[0142] Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0143] First the drawings will be described briefly.

Drawings

[0144]FIG. 1 shows a non-limiting example of a scheme for the synthesis of siNA molecules. The complementary siNA sequence strands, strand 1 and strand 2, are synthesized in tandem and are connected by a cleavable linkage, such as a nucleotide succinate or abasic succinate, which can be the same or different from the cleavable linker used for solid phase synthesis on a solid support. The synthesis can be either solid phase or solution phase, in the example shown, the synthesis is a solid phase synthesis. The synthesis is performed such that a protecting group, such as a dimethoxytrityl group, remains intact on the terminal nucleotide of the tandem oligonucleotide. Upon cleavage and deprotection of the oligonucleotide, the two siNA strands spontaneously hybridize to form a siNA duplex, which allows the purification of the duplex by utilizing the properties of the terminal protecting group, for example by applying a trityl on purification method wherein only duplexes/oligonucleotides with the terminal protecting group are isolated.

[0145]FIG. 2 shows a MALDI-TOV mass spectrum of a purified siNA duplex synthesized by a method of the invention. The two peaks shown correspond to the predicted mass of the separate siNA sequence strands. This result demonstrates that the siNA duplex generated from tandem synthesis can be purified as a single entity using a simple trityl-on purification methodology.

[0146]FIG. 3 shows a non-limiting example of HER2 protein in SK-BR-3 cells mediated siNA targeting HER2 mRNA site 2344. SK-BR-3 cells were transfected with 0.39-25 nM siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as indicated and cationic lipid (4 μg/mL). HER2 protein levels were measured 48 h post-treatment by ELISA. The ratio of HER2 protein over cell density (MTS assay) was determined for each treatment group and results are reported as normalized HER2 protein after treatment with lipid alone, active siNA or inverted control relative to untreated (UNT) cells. Results are reported as the mean of duplicate samples±1 SD.

[0147]FIG. 4 shows a non-limiting example of reduction of HER2 mRNA in SK-BR-3 cells mediated by siNA targeting HER2 mRNA site 2344. SK-BR-3 cells were transfected with 0.39-25 nM siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as indicated and cationic lipid (4 μg/mL). HER2 mRNA levels were measured 24 h post-treatment by real time RT-PCR. The ratio of HER2 mRNA over 36B4 mRNA was determined for each treatment group and results are reported as normalized HER2 mRNA after treatment with lipid alone, active siNA or inverted control relative to untreated (UNT) cells. Results are reported as the mean of triplicate samples±SD.

[0148]FIG. 5 shows a non-limiting example of antiproliferative activity of either unmodified (RPI#28268/28269) or chemically-modified (RPI#29991/29990) siNAs targeting HER2 site 2344 in SK-BR-3 cells. SK-BR-3 cells were transfected with 6.25-50 nM siNA or inverted controls (RPI#28268/28269) and (RPI#29997/29999) as indicated and cationic lipid (4 μg/mL) on days one and three. Cell proliferation was determined 96 h after treatment with lipid alone, active siNAs or inverted controls relative to untreated (UNT) cells. Results are reported as the mean of triplicate samples±SD.

[0149]FIG. 6 shows a non-limiting example of reduction of HER2 protein in SK-OV-3 cells mediated by siNA targeting HER2 mRNA site 2344. SK-BR-3 cells were transfected with 0.39-25 nM siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as indicated and cationic lipid (4 μg/mL). HER2 protein levels were measured 48 h post-treatment by ELISA. The ratio of HER2 protein over cell density (MTS assay) was determined for each treatment group and results are reported as normalized HER2 protein after treatment with lipid alone, active siNA or inverted control relative to untreated (UNT) cells. Results are reported as the mean of duplicate samples±SD.

[0150]FIG. 7 shows a non-limiting example of reduction of HER2 mRNA in SK-OV-3 cells mediated by siNA targeting HER2 mRNA site 2344. SK-BR-3 cells were transfected with 0.39-25 nM siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as indicated and cationic lipid (4 μg/mL). HER2 mRNA levels were measured 24 h post-treatment by real time RT-PCR. The ratio of HER2 mRNA over 36B4 mRNA was determined for each treatment group and results are reported as normalized HER2 mRNA after treatment with lipid alone, active siNA or inverted control relative to untreated (UNT) cells. Results are reported as the mean of triplicate samples±SD.

[0151]FIG. 8 shows a non-limiting example of reduction of HER2 mRNA in SK-OV-3 cells mediated by chemically-modified siNAs that target HER2 mRNA site 2344. SK-BR-3 cells were transfected with 6.25 or 25 nM unmodified siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as well as sets of chemically-modified siNAs as indicated and cationic lipid (4 μg/mL). A particular modified sense strand (RPI#29991) was mixed with each of four possible antisense strands (RPI#s 29990, 29994, 29995 or 29993) and cells were treated with these four sets. HER2 mRNA levels were measured 24 h post-treatment by real time RT-PCR. The ratio of HER2 mRNA over 36B4 mRNA was determined for each treatment group and results are reported as normalized HER2 mRNA after treatment with lipid alone, active siNA or inverted control, and modified sets of siNAs relative to untreated (UNT) cells. Results are reported as the mean of triplicate samples±SD.

[0152]FIG. 9 shows a non-limiting example of reduction of HER2 mRNA in SK-OV-3 cells mediated by chemically-modified siNAs that target HER2 mRNA site 2344. SK-BR-3 cells were transfected with 6.25 or 25 nM unmodified siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as well as sets of chemically-modified siNAs as indicated and cationic lipid (4 μg/mL). A particular modified sense strand (RPI#29989) was mixed with each of four possible antisense strands (RPI#s 29990, 29994, 29995 or 29993) and cells were treated with these four sets. HER2 mRNA levels were measured 24 h post-treatment by real time RT-PCR. The ratio of HER2 mRNA over 36B4 mRNA was determined for each treatment group and results are reported as normalized HER2 mRNA after treatment with lipid alone, active siNA or inverted control, and modified sets of siNAs relative to untreated (UNT) cells. Results are reported as the mean of triplicate samples ±SD.

[0153]FIG. 10 shows a non-limiting example of reduction of HER2 mRNA in SK-OV-3 cells mediated by chemically-modified siNAs that target HER2 mRNA site 2344. SK-BR-3 cells were transfected with 6.25 or 25 nM unmodified siNA (RPI#28266/28267) or the inverted control (RPI#28268/28269) as well as sets of chemically-modified siNAs as indicated and cationic lipid (4 μg/mL). A particular modified sense strand (RPI#29992) was mixed with each of four possible antisense strands (RPI#s 29990, 29994, 29995 or 29993) and cells were treated with these four sets. HER2 mRNA levels were measured 24 h post-treatment by real time RT-PCR. The ratio of HER2 mRNA over 36B4 mRNA was determined for each treatment group and results are reported as normalized HER2 mRNA after treatment with lipid alone, active siNA or inverted control, and modified sets of siNAs relative to untreated (UNT) cells. Results are reported as the mean of triplicate samples±SD.

[0154]FIG. 11 shows a non-limiting proposed mechanistic representation of target RNA degradation involved in RNAi. Double-stranded RNA (dsRNA), which is generated by RNA dependent RNA polymerase (RdRP) from foreign single-stranded RNA, for example viral, transposon, or other exogenous RNA, activates the DICER enzyme which in turn generates siNA duplexes having terminal phosphate groups (P). An active siNA complex forms which recognizes a target RNA, resulting in degradation of the target RNA by the RISC endonuclease complex or in the synthesis of additional RNA by RNA dependent RNA polymerase (RdRP), which can activate DICER and result in additional siNA molecules, thereby amplifying the RNAi response.

[0155] FIGS. 12A-F shows non-limiting examples of chemically-modified siNA constructs of the present invention. In the figure, N stands for any nucleotide (adenosine, guanosine, cytosine, uridine, or optionally thymidine, for example thymidine can be substituted in the overhanging regions designated by parenthesis (N N). Various modifications are shown for the sense and antisense strands of the siNA constructs.

[0156]FIG. 12A The sense strand comprises 21 nucleotides having four phosphorothioate 5′ and 3′-terminal internucleotide linkages, wherein the two terminal 3′-nucleotides are optionally base paired and wherein all pyrimidine nucleotides that may be present are 2′-O-methyl modified nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand comprises 21 nucleotides, wherein the two terminal 3′-nucleotides are optionally complementary to the target RNA sequence, and having one 3′-terminal phosphorothioate internucleotide linkage and four 5′-terminal phosphorothioate internucleotide linkages and wherein all pyrimidine nucleotides that may be present are 2′-deoxy-2′-fluoro modified nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein.

[0157]FIG. 12B The sense strand comprises 21 nucleotides wherein the two terminal 3′-nucleotides are optionally base paired and wherein all pyrimidine nucleotides that may be present are 2′-O-methyl modified nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand comprises 21 nucleotides, wherein the two terminal 3′-nucleotides are optionally complementary to the target RNA sequence, and wherein all pyrimidine nucleotides that may be present are 2′-deoxy-2′-fluoro modified nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein.

[0158]FIG. 12C The sense strand comprises 21 nucleotides having 5′- and 3′-terminal cap moieties wherein the two terminal 3′-nucleotides are optionally base paired and wherein all pyrimidine nucleotides that may be present are 2′-O-methyl modified nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand comprises 21 nucleotides, wherein the two terminal 3′-nucleotides are optionally complementary to the target RNA sequence, and wherein all pyrimidine nucleotides that may be present are 2′-deoxy-2′-fluoro modified nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein.

[0159]FIG. 12D The sense strand comprises 21 nucleotides having five phosphorothioate 5′ and 3′-terminal internucleotide linkages, wherein the two terminal 3′-nucleotides are optionally base paired and wherein all nucleotides are ribonucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand comprises 21 nucleotides, wherein the two terminal 3′-nucleotides are optionally complementary to the target RNA sequence, and having one 3′-terminal phosphorothioate internucleotide linkage and five 5′-terminal phosphorothioate internucleotide linkages and wherein all nucleotides are ribonucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein.

[0160]FIG. 12E The sense strand comprises 21 nucleotides wherein the two terminal 3′-nucleotides are optionally base paired and wherein all pyrimidine nucleotides that may be present are 2′-O-methyl nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand comprises 21 nucleotides all having phosphorothioate internucleotide linkages, wherein the two terminal 3′-nucleotides are optionally complementary to the target RNA sequence, and wherein all nucleotides are ribonucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein.

[0161]FIG. 12F The sense strand comprises 21 nucleotides having 5′- and 3′-terminal cap moieties, wherein the two terminal 3′-nucleotides are optionally base paired and wherein all pyrimidine nucleotides that may be present are 2′-O-methyl nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand comprises 21 nucleotides, wherein the two terminal 3′-nucleotides are optionally complementary to the target RNA sequence, and having one 3′-terminal phosphorothioate internucleotide linkage and wherein all pyrimidine nucleotides that may be present are 2′-deoxy-2′-fluoro nucleotides except for (N N) nucleotides, which can comprise naturally occurring ribonucleotides, deoxynucleotides, universal bases, or other chemical modifications described herein. The antisense strand of constructs A-F comprise sequence complementary to target RNA sequence of the invention.

[0162] FIGS. 13A-F shows non-limiting examples of specific chemically-modified siNA sequences of the invention. FIGS. 13A-F applies the chemical modifications described in FIGS. 12A-F to an HER2 siNA sequence.

[0163] FIGS. 14A-F shows non-limiting examples of specific chemically-modified siNA sequences of the invention. FIGS. 14A-F applies the chemical modifications described in FIGS. 12A-F to an HER1 siNA sequence.

[0164]FIG. 15 shows non-limiting examples of different siNA constructs of the invention. The examples shown (constructs 1, 2, and 3) have 19 representative base pairs, however, different embodiments of the invention include any number of base pairs described herein. Bracketed regions represent nucleotide overhangs, for example comprising between about 1, 2, 3, or 4 nucleotides in length, preferably about 2 nucleotides. Constructs 1 and 2 can be used independently for RNAi activity. Construct 2 can comprise a polynucleotide or non-nucleotide linker, which can optionally be designed as a biodegradable linker. In one embodiment, the loop structure shown in construct 2 can comprise a biodegradable linker that results in the formation of construct 1 in vivo and/or in vitro. In another example, construct 3 can be used to generate construct 2 under the same principle wherein a linker is used to generate the active siNA construct 2 in vivo and/or in vitro, which can optionally utilize another biodegradable linker to generate the active siNA construct 1 in vivo and/or in vitro. As such, the stability and/or activity of the siNA constructs can be modulated based on the design of the siNA construct for use in vivo or in vitro and/or in vitro.

[0165] FIGS. 16A-C is a diagrammatic representation of a scheme utilized in generating an expression cassette to generate siNA hairpin constructs.

[0166]FIG. 16A A DNA oligomer is synthesized with a 5′-restriction site (R1) sequence followed by a region having sequence identical (sense region of siNA) to a predetermined HER2 target seqeunce, wherein the sense region comprises, for example, about 19, 20, 21, or 22 nucleotides (N) in length, which is followed by a loop sequence of defined sequence (X), comprising, for example, between about 3 and 10 nucleotides.

[0167]FIG. 16B The synthetic construct is then extended by DNA polymerase to generate a hairpin structure having self complementary sequence that will result in a siNA transcript having specificity for an HER2 target sequence and having self complementary sense and antisense regions.

[0168]FIG. 16C The construct is heated (for example to about 95° C.) to linearize the sequence, thus allowing extension of a complementary second DNA strand using a primer to the 3′-restriction sequence of the first strand. The double-stranded DNA is then inserted into an appropriate vector for expression in cells. The construct can be designed such that a 3′-overhang results from the transcription, for example by engineering restriction sites and/or utilizing a poly-U termination region as described in Paul et al., 2002, Nature Biotechnology, 29, 505-508.

[0169] FIGS. 17A-C is a diagrammatic representation of a scheme utilized in generating an expression cassette to generate double-stranded siNA constructs.

[0170]FIG. 17A A DNA oligomer is synthesized with a 5′-restriction (R1) site sequence followed by a region having sequence identical (sense region of siNA) to a predetermined HER2 target seqeunce, wherein the sense region comprises, for example, about 19, 20, 21, or 22 nucleotides (N) in length, and which is followed by a 3′-restriction site (R2) which is adjacent to a loop sequence of defined sequence (X).

[0171]FIG. 17B The synthetic construct is then extended by DNA polymerase to generate a hairpin structure having self complementary sequence.

[0172]FIG. 17C The construct is processed by restriction enzymes specific to R1 and R2 to generate a double-stranded DNA which is then inserted into an appropriate vector for expression in cells. The transcription cassette is designed such that a U6 promoter region flanks each side of the dsDNA which generates the separate sense and antisense strands of the siNA. Poly T termination sequences can be added to the constructs to generate U overhangs in the resulting transcript.

[0173] FIGS. 18A-E is a diagrammatic representation of a method used to determine target sites for siNA mediated RNAi within a particular target nucleic acid sequence, such as messenger RNA.

[0174]FIG. 18A A pool of siNA oligonucleotides are synthesized wherein the antisense region of the siNA constructs has complementarity to target sites across the target nucleic acid sequence, and wherein the sense region comprises sequence complementary to the antisense region of the siNA.

[0175] FIGS. 18B-C The sequences are pooled and are inserted into vectors such that transfection of a vector into cells results in the expression of the siNA (FIG. 18C).

[0176]FIG. 18D Cells are sorted based on phenotypic change that is associated with modulation of the target nucleic acid sequence.

[0177]FIG. 18E The siNA is isolated from the sorted cells and is sequenced to identify efficacious target sites within the target nucleic acid sequence.

[0178]FIG. 19 shows non-limiting examples of different stabilization chemistries (1-10) that can be used, for example, to stabilize the 3′-end of siNA sequences of the invention, including (1) [3-3′]-inverted deoxyribose; (2) deoxyribonucleotide; (3) [5′-3′]-3′-deoxyribonucleotide; (4) [5′-3′]-ribonucleotide; (5) [5′-3′]-3′-O-methyl ribonucleotide; (6) 3′-glyceryl; (7) [3′-5′]-3′-deoxyribonucleotide; (8) [3′-3′]-deoxyribonucleotide; (9) [5′-2′]-deoxyribonucleotide; and (10) [5-3′]-dideoxyribonucleotide. In addition to modified and unmodified backbone chemistries indicated in the figure, these chemistries can be combined with different backbone modifications as described herein, for example, backbone modifications having Formula I. In addition, the 2′-deoxy nucleotide shown 5′ to the terminal modifications shown can be another modified or unmodified nucleotide or non-nucleotide described herein, for example modifications having Formulae II, III, IV, V, or VI.

[0179] Mechanism of action of Nucleic Acid Molecules of the Invention

[0180] The discussion that follows discusses the proposed mechanism of RNA interference mediated by short interfering RNA as is presently known, and is not meant to be limiting and is not an admission of prior art. Applicant demonstrates herein that chemically-modified short interfering nucleic acids possess similar or improved capacity to mediate RNAi as do siRNA molecules and are expected to possess improved stability and activity in vivo; therefore, this discussion is not meant to be limiting only to siRNA and can be applied to siNA as a whole. By “improved capacity to mediate RNAi” is meant to include RNAi activity measured in vitro and/or in vivo where the RNAi activity is a reflection of both the ability of the siNA to mediate RNAi and the stability of the siRNAs of the invention. In this invention, the product of these activities can be increased in vitro and/or in vivo compared to an all RNA siRNA or an siNA containing a plurality of ribonucleotides. In some cases, the activity or stability of the siNA molecule can be decreased (i.e., less than ten-fold), but the overall activity of the siNA molecule is enhanced, in vitro and/or in vivo.

[0181] RNA interference refers to the process of sequence specific post-transcriptional gene silencing in animals mediated by short interfering nucleic acid (siNA) or short interfering RNAs (siRNA) (Fire et al., 1998, Nature, 391, 806). The corresponding process in plants is commonly referred to as post-transcriptional gene silencing or RNA silencing and is also referred to as quelling in fungi. The process of post-transcriptional gene silencing is thought to be an evolutionarily conserved cellular defense mechanism used to prevent the expression of foreign genes which is commonly shared by diverse flora and phyla (Fire et al., 1999, Trends Genet., 15, 358). Such protection from foreign gene expression may have evolved in response to the production of double-stranded RNAs (dsRNA) derived from viral infection or the random integration of transposon elements into a host genome via a cellular response that specifically destroys homologous single-stranded RNA or viral genomic RNA. The presence of dsRNA in cells triggers the RNAi response though a mechanism that has yet to be fully characterized. This mechanism appears to be different from the interferon response that results from dsRNA mediated activation of protein kinase PKR and 2′,5′-oligoadenylate synthetase resulting in non-specific cleavage of mRNA by ribonuclease L.

[0182] The presence of long dsRNAs in cells stimulates the activity of a ribonuclease III enzyme referred to as dicer. Dicer is involved in the processing of the dsRNA into short pieces of dsRNA known as short interfering RNAs (siRNA) (Berstein et al., 2001, Nature, 409, 363). Short interfering RNAs derived from dicer activity are typically about 21 to about 23 nucleotides in length and comprise about 19 base pair duplexes. Dicer has also been implicated in the excision of about 21 and about 22 nucleotide small temporal RNAs (stRNA) from precursor RNA of conserved structure that are implicated in translational control (Hutvagner et al., 2001, Science, 293, 834). The RNAi response also features an endonuclease complex containing a siRNA, commonly referred to as an RNA-induced silencing complex (RISC), which mediates cleavage of single-stranded RNA having sequence homologous to the siRNA. Cleavage of the target RNA takes place in the middle of the region complementary to the guide sequence of the siRNA duplex (Elbashir et al., 2001, Genes Dev., 15, 188).

[0183] Short interfering RNA mediated RNAi has been studied in a variety of systems. Fire et al, 1998, Nature, 391, 806, were the first to observe RNAi in C. Elegans. Wianny and Goetz, 1999, Nature Cell Biol., 2, 70, describe RNAi mediated by dsRNA in mouse embryos. Hammond et al., 2000, Nature, 404, 293, describe RNAi in Drosophila cells transfected with dsRNA. Elbashir et al., 2001, Nature, 411, 494, describe RNAi induced by introduction of duplexes of synthetic 21-nucleotide RNAs in cultured mammalian cells including human embryonic kidney and HeLa cells. Recent work in Drosophila embryonic lysates has revealed certain requirements for siRNA length, structure, chemical composition, and sequence that are essential to mediate efficient RNAi activity. These studies have shown that 21 nucleotide siRNA duplexes are most active when containing two di-nucleotide 3′-terminal nucleotide overhangs. Furthermore, substitution of one or both siRNA strands with 2′-deoxy or 2′-O-methyl nucleotides abolishes RNAi activity, whereas substitution of 3′-terminal siRNA nucleotides with deoxy nucleotides was shown to be tolerated. Mismatch sequences in the center of the siRNA duplex were also shown to abolish RNAi activity. In addition, these studies also indicate that the position of the cleavage site in the target RNA is defined by the 5′-end of the siRNA guide sequence rather than the 3′-end (Elbashir et al., 2001, EMBO J, 20, 6877). Other studies have indicated that a 5′-phosphate on the target-complementary strand of a siRNA duplex is required for siRNA activity and that ATP is utilized to maintain the 5′-phosphate moiety on the siRNA (Nykanen et al., 2001, Cell, 107, 309), however siRNA molecules lacking a 5′-phosphate are active when introduced exogenously, suggesting that 5′-phosphorylation of siRNA constructs may occur in vivo.

[0184] Synthesis of Nucleic acid Molecules

[0185] Synthesis of nucleic acids greater than 100 nucleotides in length is difficult using automated methods, and the therapeutic cost of such molecules is prohibitive. In this invention, small nucleic acid motifs (“small” refers to nucleic acid motifs no more than 100 nucleotides in length, preferably no more than 80 nucleotides in length, and most preferably no more than 50 nucleotides in length; e.g., individual siNA oligonucleotide sequences or siNA sequences synthesized in tandem) are preferably used for exogenous delivery. The simple structure of these molecules increases the ability of the nucleic acid to invade targeted regions of protein and/or RNA structure. Exemplary molecules of the instant invention are chemically synthesized, and others can similarly be synthesized.

[0186] Oligonucleotides (e.g., certain modified oligonucleotides or portions of oligonucleotides lacking ribonucleotides) are synthesized using protocols known in the art, for example as described in Caruthers et al., 1992, Methods in Enzymology 211, 3-19, Thompson et al., International PCT Publication No. WO 99/54459, Wincott et al., 1995, Nucleic Acids Res. 23, 2677-2684, Wincott et al., 1997, Methods Mol. Bio., 74, 59, Brennan et al., 1998, Biotechnol Bioeng., 61, 33-45, and Brennan, U.S. Pat. No. 6,001,311. All of these references are incorporated herein by reference. The synthesis of oligonucleotides makes use of common nucleic acid protecting and coupling groups, such as dimethoxytrityl at the 5′-end, and phosphoramidites at the 3′-end. In a non-limiting example, small scale syntheses are conducted on a 394 Applied Biosystems, Inc. synthesizer using a 0.2 μmol scale protocol with a 2.5 min coupling step for 2′-O-methylated nucleotides and a 45 sec coupling step for 2′-deoxy nucleotides or 2′-deoxy-2′-fluoro nucleotides. Table VI outlines the amounts and the contact times of the reagents used in the synthesis cycle. Alternatively, syntheses at the 0.2 μmol scale can be performed on a 96-well plate synthesizer, such as the instrument produced by Protogene (Palo Alto, Calif.) with minimal modification to the cycle. A 33-fold excess (60 μL of 0.11 M=6.6 μmol) of 2′-O-methyl phosphoramidite and a 105-fold excess of S-ethyl tetrazole (60 μL of 0.25 M=15 μmol) can be used in each coupling cycle of 2′-O-methyl residues relative to polymer-bound 5′-hydroxyl. A 22-fold excess (40 μL of 0.11 M=4.4 μmol) of deoxy phosphoramidite and a 70-fold excess of S-ethyl tetrazole (40 μL of 0.25 M=10 μmol) can be used in each coupling cycle of deoxy residues relative to polymer-bound 5′-hydroxyl. Average coupling yields on the 394 Applied Biosystems, Inc. synthesizer, determined by colorimetric quantitation of the trityl fractions, are typically 97.5-99%. Other oligonucleotide synthesis reagents for the 394 Applied Biosystems, Inc. synthesizer include the following: detritylation solution is 3% TCA in methylene chloride (ABI); capping is performed with 16% N-methyl imidazole in THF (ABI) and 10% acetic anhydride/10% 2,6-lutidine in THF (ABI); and oxidation solution is 16.9 mM I₂, 49 mM pyridine, 9% water in THF (PERSEPTIVE™). Burdick & Jackson Synthesis Grade acetonitrile is used directly from the reagent bottle. S-Ethyltetrazole solution (0.25 M in acetonitrile) is made up from the solid obtained from American International Chemical, Inc. Alternately, for the introduction of phosphorothioate linkages, Beaucage reagent (3H-1,2-Benzodithiol-3-one 1,1-dioxide, 0.05 M in acetonitrile) is used.

[0187] Deprotection of the DNA-based oligonucleotides is performed as follows: the polymer-bound trityl-on oligoribonucleotide is transferred to a 4 mL glass screw top vial and suspended in a solution of 40% aq. methylamine (1 mL) at 65° C. for 10 min. After cooling to −20° C., the supernatant is removed from the polymer support. The support is washed three times with 1.0 mL of EtOH:MeCN:H2O/3:1:1, vortexed and the supernatant is then added to the first supernatant. The combined supernatants, containing the oligoribonucleotide, are dried to a white powder.

[0188] The method of synthesis used for RNA including certain siNA molecules of the invention follows the procedure as described in Usman et al., 1987, J. Am. Chem. Soc., 109, 7845; Scaringe et al., 1990, Nucleic Acids Res., 18, 5433; and Wincott et al., 1995, Nucleic Acids Res. 23, 2677-2684 Wincott et al., 1997, Methods Mol. Bio., 74, 59, and makes use of common nucleic acid protecting and coupling groups, such as dimethoxytrityl at the 5′-end, and phosphoramidites at the 3′-end. In a non-limiting example, small scale syntheses are conducted on a 394 Applied Biosystems, Inc. synthesizer using a 0.2 μmol scale protocol with a 7.5 min coupling step for alkylsilyl protected nucleotides and a 2.5 min coupling step for 2′-O-methylated nucleotides. Table VI outlines the amounts and the contact times of the reagents used in the synthesis cycle. Alternatively, syntheses at the 0.2 μmol scale can be done on a 96-well plate synthesizer, such as the instrument produced by Protogene (Palo Alto, Calif.) with minimal modification to the cycle. A 33-fold excess (60 μL of 0.11 M=6.6 μmol) of 2′-O-methyl phosphoramidite and a 75-fold excess of S-ethyl tetrazole (60 μL of 0.25 M=15 μmol) can be used in each coupling cycle of 2′-O-methyl residues relative to polymer-bound 5′-hydroxyl. A 66-fold excess (120 μL of 0.11 M=13.2 μmol) of alkylsilyl (ribo) protected phosphoramidite and a 150-fold excess of S-ethyl tetrazole (120 μL of 0.25 M=30 μmol) can be used in each coupling cycle of ribo residues relative to polymer-bound 5′-hydroxyl. Average coupling yields on the 394 Applied Biosystems, Inc. synthesizer, determined by colorimetric quantitation of the trityl fractions, are typically 97.5-99%. Other oligonucleotide synthesis reagents for the 394 Applied Biosystems, Inc. synthesizer include the following: detritylation solution is 3% TCA in methylene chloride (ABI); capping is performed with 16% N-methyl imidazole in THF (ABI) and 10% acetic anhydride/10% 2,6-lutidine in THF (ABI); oxidation solution is 16.9 mM I₂, 49 mM pyridine, 9% water in THF (PERSEPTIVE™). Burdick & Jackson Synthesis Grade acetonitrile is used directly from the reagent bottle. S-Ethyltetrazole solution (0.25 M in acetonitrile) is made up from the solid obtained from American International Chemical, Inc. Alternately, for the introduction of phosphorothioate linkages, Beaucage reagent (3H-1,2-Benzodithiol-3-one 1,1-dioxide 0.05 M in acetonitrile) is used.

[0189] Deprotection of the RNA is performed using either a two-pot or one-pot protocol. For the two-pot protocol, the polymer-bound trityl-on oligoribonucleotide is transferred to a 4 mL glass screw top vial and suspended in a solution of 40% aq. methylamine (1 mL) at 65° C. for 10 min. After cooling to −20° C., the supernatant is removed from the polymer support. The support is washed three times with 1.0 mL of EtOH:MeCN:H2O/3:1:1, vortexed and the supernatant is then added to the first supernatant. The combined supernatants, containing the oligoribonucleotide, are dried to a white powder. The base deprotected oligoribonucleotide is resuspended in anhydrous TEA/HF/NMP solution (300 μL of a solution of 1.5 mL N-methylpyrrolidinone, 750 μL TEA and 1 mL TEA·3HF to provide a 1.4 M HF concentration) and heated to 65° C. After 1.5 h, the oligomer is quenched with 1.5 M NH₄HCO₃.

[0190] Alternatively, for the one-pot protocol, the polymer-bound trityl-on oligoribonucleotide is transferred to a 4 mL glass screw top vial and suspended in a solution of 33% ethanolic methylamine/DMSO: 1/1 (0.8 mL) at 65° C. for 15 min. The vial is brought to r.t. TEA·3HF (0.1 mL) is added and the vial is heated at 65° C. for 15 min. The sample is cooled at −20° C. and then quenched with 1.5 M NH₄HCO₃.

[0191] For purification of the trityl-on oligomers, the quenched NH₄HCO₃ solution is loaded onto a C-18 containing cartridge that had been prewashed with acetonitrile followed by 50 mM TEAA. After washing the loaded cartridge with water, the RNA is detritylated with 0.5% TFA for 13 min. The cartridge is then washed again with water, salt exchanged with 1 M NaCl and washed with water again. The oligonucleotide is then eluted with 30% acetonitrile.

[0192] The average stepwise coupling yields are typically >98% (Wincott et al., 1995 Nucleic Acids Res. 23, 2677-2684). Those of ordinary skill in the art will recognize that the scale of synthesis can be adapted to be larger or smaller than the example described above including but not limited to 96-well format, all that is important is the ratio of chemicals used in the reaction.

[0193] Alternatively, the nucleic acid molecules of the present invention can be synthesized separately and joined together post-synthetically, for example, by ligation (Moore et al., 1992, Science 256, 9923; Draper et al., International PCT Publication No. WO 93/23569; Shabarova et al., 1991, Nucleic Acids Research 19, 4247; Bellon et al., 1997, Nucleosides & Nucleotides, 16, 951; Bellon et al., 1997, Bioconjugate Chem. 8, 204), or by hybridization following synthesis and/or deprotection.

[0194] The siNA molecules of the invention can also be synthesized via a tandem synthesis methodology as described in Example 1 herein, wherein both siNA strands are synthesized as a contiguous oligonucleotide sequence separated by a cleavable linker which is subsequently cleaved to provide separate siNA sequences that hybridize and permit purification of the siNA duplex. The tandem synthesis of siNA as described herein can be readily adapted to both multiwell/multiplate synthesis platforms such as 96 well or similarly larger multi-well platforms. The tandem synthesis of siNA as described herein can also be readily adapted to large scale synthesis platforms employing batch reactors, synthesis columns and the like.

[0195] The nucleic acid molecules of the present invention can be modified extensively to enhance stability by modification with nuclease resistant groups, for example, 2′-amino, 2′-C-allyl, 2′-flouro, 2′-O-methyl, 2′-H (for a review see Usman and Cedergren, 1992, TIBS 17, 34; Usman et al., 1994, Nucleic Acids Symp. Ser. 31, 163). siNA constructs can be purified by gel electrophoresis using general methods or can be purified by high pressure liquid chromatography (HPLC; see Wincott et al., supra, the totality of which is hereby incorporated herein by reference) and re-suspended in water.

[0196] In another aspect of the invention, siNA molecules of the invention are expressed from transcription units inserted into DNA or RNA vectors. The recombinant vectors can be DNA plasmids or viral vectors. siNA expressing viral vectors can be constructed based on, but not limited to, adeno-associated virus, retrovirus, adenovirus, or alphavirus. The recombinant vectors capable of expressing the siNA molecules can be delivered as described herein, and persist in target cells. Alternatively, viral vectors can be used that provide for transient expression of siNA molecules.

[0197] The sequences of the siNA constructs that are chemically synthesized, useful in this study, are shown in Table II, III, V and/or FIGS. 12-14. Similarly, the siNA construct sequences listed in the Tables can be formed of ribonucleotides or other nucleotides or non-nucleotides as described herein.

[0198] Optimizing Activity of the nucleic acid molecule of the invention.

[0199] Chemically synthesizing nucleic acid molecules with modifications (base, sugar and/or phosphate) can prevent their degradation by serum ribonucleases, which can increase their potency (see e.g., Eckstein et al., International Publication No. WO 92/07065; Perrault et al., 1990 Nature 344, 565; Pieken et al., 1991, Science 253, 314; Usman and Cedergren, 1992, Trends in Biochem. Sci. 17, 334; Usman et al., International Publication No. WO 93/15187; and Rossi et al., International Publication No. WO 91/03162; Sproat, U.S. Pat. No. 5,334,711; Gold et al., U.S. Pat. No. 6,300,074; and Burgin et al., supra; all of which are incorporated by reference herein). All of the above references describe various chemical modifications that can be made to the base, phosphate and/or sugar moieties of the nucleic acid molecules described herein. Modifications that enhance their efficacy in cells, and removal of bases from nucleic acid molecules to shorten oligonucleotide synthesis times and reduce chemical requirements are desired.

[0200] There are several examples in the art describing sugar, base and phosphate modifications that can be introduced into nucleic acid molecules with significant enhancement in their nuclease stability and efficacy. For example, oligonucleotides are modified to enhance stability and/or enhance biological activity by modification with nuclease resistant groups, for example, 2′-amino, 2′-C-allyl, 2′-flouro, 2′-O-methyl, 2′-O-allyl, and/or 2′-H, nucleotide base modifications (for a review see Usman and Cedergren, 1992, TIBS., 17, 34; Usman et al., 1994, Nucleic Acids Symp. Ser. 31, 163; Burgin et al., 1996, Biochemistry, 35, 14090). Sugar modification of nucleic acid molecules have been extensively described in the art (see Eckstein et al., International Publication PCT No. WO 92/07065; Perrault et al. Nature, 1990, 344, 565-568; Pieken et al. Science, 1991, 253, 314-317; Usman and Cedergren, Trends in Biochem. Sci., 1992, 17, 334-339; Usman et al. International Publication PCT No. WO 93/15187; Sproat, U.S. Pat. No. 5,334,711 and Beigelman et al., 1995, J. Biol. Chem., 270, 25702; Beigelman et al., International PCT publication No. WO 97/26270; Beigelman et al., U.S. Pat. No. 5,716,824; Usman et al., U.S. Pat. No. 5,627,053; Woolf et al., International PCT Publication No. WO 98/13526; Thompson et al., U.S. Ser. No. 60/082,404 filed on Apr. 20, 1998; Karpeisky et al., 1998, Tetrahedron Lett., 39, 1131; Earnshaw and Gait, 1998, Biopolymers (Nucleic Acid Sciences), 48, 39-55; Verma and Eckstein, 1998, Annu. Rev. Biochem., 67, 99-134; and Burlina et al., 1997, Bioorg. Med. Chem., 5, 1999-2010; all of the references are hereby incorporated in their totality by reference herein). Such publications describe general methods and strategies to determine the location of incorporation of sugar, base and/or phosphate modifications and the like into nucleic acid molecules without modulating catalysis, and are incorporated by reference herein. In view of such teachings, similar modifications can be used as described herein to modify the siNA nucleic acid molecules of the instant invention so long as the ability of siNA to promote RNAi is cells is not significantly inhibited.

[0201] While chemical modification of oligonucleotide internucleotide linkages with phosphorothioate, phosphorothioate, and/or 5′-methylphosphonate linkages improves stability, excessive modifications can cause some toxicity or decreased activity. Therefore, when designing nucleic acid molecules, the amount of these internucleotide linkages should be minimized. The reduction in the concentration of these linkages should lower toxicity, resulting in increased efficacy and higher specificity of these molecules.

[0202] Short interfering nucleic acid (siNA) molecules having chemical modifications that maintain or enhance activity are provided. Such a nucleic acid is also generally more resistant to nucleases than an unmodified nucleic acid. Accordingly, the in vitro and/or in vivo activity should not be significantly lowered. In cases in which modulation is the goal, therapeutic nucleic acid molecules delivered exogenously should optimally be stable within cells until translation of the target RNA has been modulated long enough to reduce the levels of the undesirable protein. This period of time varies between hours to days depending upon the disease state. Improvements in the chemical synthesis of RNA and DNA (Wincott et al., 1995, Nucleic Acids Res. 23, 2677; Caruthers et al., 1992, Methods in Enzymology 211,3-19 (incorporated by reference herein)) have expanded the ability to modify nucleic acid molecules by introducing nucleotide modifications to enhance their nuclease stability, as described above.

[0203] In one embodiment, nucleic acid molecules of the invention include one or more G-clamp nucleotides. A G-clamp nucleotide is a modified cytosine analog wherein the modifications confer the ability to hydrogen bond both Watson-Crick and Hoogsteen faces of a complementary guanine within a duplex, see for example Lin and Matteucci, 1998, J. Am. Chem. Soc., 120, 8531-8532. A single G-clamp analog substitution within an oligonucleotide can result in substantially enhanced helical thermal stability and mismatch discrimination when hybridized to complementary oligonucleotides. The inclusion of such nucleotides in nucleic acid molecules of the invention results in both enhanced affinity and specificity to nucleic acid targets, complementary sequences, or template strands. In another embodiment, nucleic acid molecules of the invention include one or more LNA “locked nucleic acid” nucleotides such as a 2′, 4′-C mythylene bicyclo nucleotide (see for example Wengel et al., International PCT Publication No. WO 00/66604 and WO 99/14226).

[0204] In another embodiment, the invention features conjugates and/or complexes of siNA molecules of the invention. Such conjugates and/or complexes can be used to facilitate delivery of siNA molecules into a biological system, such as a cell. The conjugates and complexes provided by the instant invention can impart therapeutic activity by transferring therapeutic compounds across cellular membranes, altering the pharmacokinetics, and/or modulating the localization of nucleic acid molecules of the invention. The present invention encompasses the design and synthesis of novel conjugates and complexes for the delivery of molecules, including, but not limited to, small molecules, lipids, phospholipids, nucleosides, nucleotides, nucleic acids, antibodies, toxins, negatively charged polymers and other polymers, for example proteins, peptides, hormones, carbohydrates, polyethylene glycols, or polyamines, across cellular membranes. In general, the transporters described are designed to be used either individually or as part of a multi-component system, with or without degradable linkers. These compounds are expected to improve delivery and/or localization of nucleic acid molecules of the invention into a number of cell types originating from different tissues, in the presence or absence of serum (see Sullenger and Cech, U.S. Pat. No. 5,854,038). Conjugates of the molecules described herein can be attached to biologically active molecules via linkers that are biodegradable, such as biodegradable nucleic acid linker molecules.

[0205] The term “biodegradable nucleic acid linker molecule” as used herein, refers to a nucleic acid molecule that is designed as a biodegradable linker to connect one molecule to another molecule, for example, a biologically active molecule. The stability of the biodegradable nucleic acid linker molecule can be modulated by using various combinations of ribonucleotides, deoxyribonucleotides, and chemically-modified nucleotides, for example, 2′-O-methyl, 2′-fluoro, 2′-amino, 2′-O-amino, 2′-C-allyl, 2′-O-allyl, and other 2′-modified or base-modified nucleotides. The biodegradable nucleic acid linker molecule can be a dimer, trimer, tetramer or longer nucleic acid molecule, for example, an oligonucleotide of about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleotides in length, or can comprise a single nucleotide with a phosphorus-based linkage, for example, a phosphoramidate or phosphodiester linkage. The biodegradable nucleic acid linker molecule can also comprise nucleic acid backbone, nucleic acid sugar, or nucleic acid base modifications.

[0206] The term “biodegradable” as used herein, refers to degradation in a biological system, for example enzymatic degradation or chemical degradation.

[0207] The term “biologically active molecule” as used herein, refers to compounds or molecules that are capable of eliciting or modifying a biological response in a system. Non-limiting examples of biologically active siNA molecules either alone or in combination with othe molecules contemplated by the instant invention include therapeutically active molecules such as antibodies, hormones, antivirals, peptides, proteins, chemotherapeutics, small molecules, vitamins, co-factors, nucleosides, nucleotides, oligonucleotides, enzymatic nucleic acids, antisense nucleic acids, triplex forming oligonucleotides, 2,5-A chimeras, siNA, dsRNA, allozymes, aptamers, decoys and analogs thereof. Biologically active molecules of the invention also include molecules capable of modulating the pharmacokinetics and/or pharmacodynamics of other biologically active molecules, for example, lipids and polymers such as polyamines, polyamides, polyethylene glycol and other polyethers.

[0208] The term “phospholipid” as used herein, refers to a hydrophobic molecule comprising at least one phosphorus group. For example, a phospholipid can comprise a phosphorus-containing group and saturated or unsaturated alkyl group, optionally substituted with OH, COOH, oxo, amine, or substituted or unsubstituted aryl groups.

[0209] Therapeutic nucleic acid molecules (e.g., siNA molecules) delivered exogenously optimally are stable within cells until reverse trascription of the RNA has been modulated long enough to reduce the levels of the RNA transcript. The nucleic acid molecules are resistant to nucleases in order to function as effective intracellular therapeutic agents. Improvements in the chemical synthesis of nucleic acid molecules described in the instant invention and in the art have expanded the ability to modify nucleic acid molecules by introducing nucleotide modifications to enhance their nuclease stability as described above.

[0210] In yet another embodiment, siNA molecules having chemical modifications that maintain or enhance enzymatic activity of proteins involved in RNAi are provided. Such nucleic acids are also generally more resistant to nucleases than unmodified nucleic acids. Thus, in vitro and/or in vivo the activity should not be significantly lowered.

[0211] Use of the nucleic acid-based molecules of the invention will lead to better treatment of the disease progression by affording the possibility of combination therapies (e.g., multiple siNA molecules targeted to different genes; nucleic acid molecules coupled with known small molecule modulators; or intermittent treatment with combinations of molecules, including different motifs and/or other chemical or biological molecules). The treatment of patients with siNA molecules can also include combinations of different types of nucleic acid molecules, such as enzymatic nucleic acid molecules (ribozymes), allozymes, antisense molecules, 2,5-A oligoadenylate, decoys, aptamers etc.

[0212] In another aspect a siNA molecule of the invention comprises one or more 5′ and/or a 3′-cap structure, for example on only the sense siNA strand, antisense siNA strand, or both siNA strands.

[0213] By “cap structure” is meant chemical modifications, which have been incorporated at either terminus of the oligonucleotide (see, for example, Adamic et al., U.S. Pat. No. 5,998,203, incorporated by reference herein). These terminal modifications protect the nucleic acid molecule from exonuclease degradation, and can help in delivery and/or localization within a cell. The cap can be present at the 5′-terminus (5′-cap) or at the 3′-terminal (3′-cap) or can be present on both termini. In non-limiting examples: the 5′-cap is selected from the group comprising inverted abasic residue (moiety); 4,5′-methylene nucleotide; 1-(beta-D-erythrofuranosyl) nucleotide, 4′-thio nucleotide; carbocyclic nucleotide; 1,5-anhydrohexitol nucleotide; L-nucleotides; alpha-nucleotides; modified base nucleotide; phosphorodithioate linkage; threo-pentofuranosyl nucleotide; acyclic 3′,4′-seco nucleotide; acyclic 3,4-dihydroxybutyl nucleotide; acyclic 3,5-dihydroxypentyl nucleotide, 3′-3′-inverted nucleotide moiety; 3′-3′-inverted abasic moiety; 3′-2′-inverted nucleotide moiety; 3′-2′-inverted abasic moiety; 1,4-butanediol phosphate; 3′-phosphoramidate; hexylphosphate; aminohexyl phosphate; 3′-phosphate; 3′-phosphorothioate; phosphorodithioate; or bridging or non-bridging methylphosphonate moiety.

[0214] In yet another preferred embodiment, the 3′-cap is selected from a group comprising, 4′,5′-methylene nucleotide; 1-(beta-D-erythrofuranosyl) nucleotide; 4′-thio nucleotide, carbocyclic nucleotide; 5′-amino-alkyl phosphate; 1,3-diamino-2-propyl phosphate; 3-aminopropyl phosphate; 6-aminohexyl phosphate; 1,2-aminododecyl phosphate; hydroxypropyl phosphate; 1,5-anhydrohexitol nucleotide; L-nucleotide; alpha-nucleotide; modified base nucleotide; phosphorodithioate; threo-pentofuranosyl nucleotide; acyclic 3′,4′-seco nucleotide; 3,4-dihydroxybutyl nucleotide; 3,5-dihydroxypentyl nucleotide, 5′-5′-inverted nucleotide moiety; 5′-5′-inverted abasic moiety; 5′-phosphoramidate; 5′-phosphorothioate; 1,4-butanediol phosphate; 5′-amino; bridging and/or non-bridging 5′-phosphoramidate, phosphorothioate and/or phosphorodithioate, bridging or non bridging methylphosphonate and 5′-mercapto moieties (for more details see Beaucage and Iyer, 1993, Tetrahedron 49, 1925; incorporated by reference herein).

[0215] By the term “non-nucleotide” is meant any group or compound which can be incorporated into a nucleic acid chain in the place of one or more nucleotide units, including either sugar and/or phosphate substitutions, and allows the remaining bases to exhibit their enzymatic activity. The group or compound is abasic in that it does not contain a commonly recognized nucleotide base, such as adenosine, guanine, cytosine, uracil or thymine and therefore lacks a base at the 1′-position.

[0216] An “alkyl” group refers to a saturated aliphatic hydrocarbon, including straight-chain, branched-chain, and cyclic alkyl groups. Preferably, the alkyl group has 1 to 12 carbons. More preferably, it is a lower alkyl of from 1 to 7 carbons, more preferably 1 to 4 carbons. The alkyl group can be substituted or unsubstituted. When substituted the substituted group(s) is preferably, hydroxyl, cyano, alkoxy, ═O, ═S, NO₂ or N(CH₃)₂, amino, or SH. The term also includes alkenyl groups that are unsaturated hydrocarbon groups containing at least one carbon-carbon double bond, including straight-chain, branched-chain, and cyclic groups. Preferably, the alkenyl group has 1 to 12 carbons. More preferably, it is a lower alkenyl of from 1 to 7 carbons, more preferably 1 to 4 carbons. The alkenyl group can be substituted or unsubstituted. When substituted the substituted group(s) is preferably, hydroxyl, cyano, alkoxy, ═O, ═S, NO₂, halogen, N(CH₃)₂, amino, or SH. The term “alkyl” also includes alkynyl groups that have an unsaturated hydrocarbon group containing at least one carbon-carbon triple bond, including straight-chain, branched-chain, and cyclic groups. Preferably, the alkynyl group has 1 to 12 carbons. More preferably, it is a lower alkynyl of from 1 to 7 carbons, more preferably 1 to 4 carbons. The alkynyl group can be substituted or unsubstituted. When substituted the substituted group(s) is preferably, hydroxyl, cyano, alkoxy, ═O, ═S, NO₂ or N(CH₃)₂, amino or SH.

[0217] Such alkyl groups can also include aryl, alkylaryl, carbocyclic aryl, heterocyclic aryl, amide and ester groups. An “aryl” group refers to an aromatic group that has at least one ring having a conjugated pi electron system and includes carbocyclic aryl, heterocyclic aryl and biaryl groups, all of which can be optionally substituted. The preferred substituent(s) of aryl groups are halogen, trihalomethyl, hydroxyl, SH, OH, cyano, alkoxy, alkyl, alkenyl, alkynyl, and amino groups. An “alkylaryl” group refers to an alkyl group (as described above) covalently joined to an aryl group (as described above). Carbocyclic aryl groups are groups wherein the ring atoms on the aromatic ring are all carbon atoms. The carbon atoms are optionally substituted. Heterocyclic aryl groups are groups having from 1 to 3 heteroatoms as ring atoms in the aromatic ring and the remainder of the ring atoms are carbon atoms. Suitable heteroatoms include oxygen, sulfur, and nitrogen, and include furanyl, thienyl, pyridyl, pyrrolyl, N-lower alkyl pyrrolo, pyrimidyl, pyrazinyl, imidazolyl and the like, all optionally substituted. An “amide” refers to an —C(O)—NH—R, where R is either alkyl, aryl, alkylaryl or hydrogen. An “ester” refers to an —C(O)—OR′, where R is either alkyl, aryl, alkylaryl or hydrogen.

[0218] By “nucleotide” as used herein is as recognized in the art to include natural bases (standard), and modified bases well known in the art. Such bases are generally located at the 1′ position of a nucleotide sugar moiety. Nucleotides generally comprise a base, sugar and a phosphate group. The nucleotides can be unmodified or modified at the sugar, phosphate and/or base moiety, (also referred to interchangeably as nucleotide analogs, modified nucleotides, non-natural nucleotides, non-standard nucleotides and other; see, for example, Usman and McSwiggen, supra; Eckstein et al., International PCT Publication No. WO 92/07065; Usman et al., International PCT Publication No. WO 93/15187; Uhlman & Peyman, supra, all are hereby incorporated by reference herein). There are several examples of modified nucleic acid bases known in the art as summarized by Limbach et al., 1994, Nucleic Acids Res. 22, 2183. Some of the non-limiting examples of base modifications that can be introduced into nucleic acid molecules include, inosine, purine, pyridin-4-one, pyridin-2-one, phenyl, pseudouracil, 2, 4, 6-trimethoxy benzene, 3-methyl uracil, dihydrouridine, naphthyl, aminophenyl, 5-alkylcytidines (e.g., 5-methylcytidine), 5-alkyluridines (e.g., ribothymidine), 5-halouridine (e.g., 5-bromouridine) or 6-azapyrimidines or 6-alkylpyrimidines (e.g. 6-methyluridine), propyne, and others (Burgin et al., 1996, Biochemistry, 35, 14090; Uhlman & Peyman, supra). By “modified bases” in this aspect is meant nucleotide bases other than adenine, guanine, cytosine and uracil at 1′ position or their equivalents.

[0219] In one embodiment, the invention features modified siNA molecules, with phosphate backbone modifications comprising one or more phosphorothioate, phosphorodithioate, methylphosphonate, morpholino, amidate carbamate, carboxymethyl, acetamidate, polyamide, sulfonate, sulfonamide, sulfamate, formacetal, thioformacetal, and/or alkylsilyl, substitutions. For a review of oligonucleotide backbone modifications, see Hunziker and Leumann, 1995, Nucleic Acid Analogues: Synthesis and Properties, in Modern Synthetic Methods, VCH, 331-417, and Mesmaeker et al., 1994, Novel Backbone Replacements for Oligonucleotides, in Carbohydrate Modifications in Antisense Research, ACS, 24-39.

[0220] By “abasic” is meant sugar moieties lacking a base or having other chemical groups in place of a base at the 1′ position, see for example Adamic et al., U.S. Pat. No. 5,998,203.

[0221] By “unmodified nucleoside” is meant one of the bases adenine, cytosine, guanine, thymine, uracil joined to the 1′ carbon of β-D-ribo-furanose.

[0222] By “modified nucleoside” is meant any nucleotide base that contains a modification in the chemical structure of an unmodified nucleotide base, sugar and/or phosphate.

[0223] In connection with 2′-modified nucleotides as described for the present invention, by “amino” is meant 2′—NH₂ or 2′—O—NH_(2,) which can be modified or unmodified. Such modified groups are described, for example, in Eckstein et al., U.S. Pat. No. 5,672,695 and Matulic-Adamic et al., U.S. Pat. No. 6,248,878, which are both incorporated by reference in their entireties.

[0224] Various modifications to nucleic acid siNA structure can be made to enhance the utility of these molecules. Such modifications will enhance shelf-life, half-life in vitro, stability, and ease of introduction of such oligonucleotides to the target site, e.g., to enhance penetration of cellular membranes, and confer the ability to recognize and bind to targeted cells.

[0225] Administration of Nucleic Acid Molecules

[0226] A siNA molecule of the invention can be adapted for use to treat, for example, cancer and any other indications that can respond to the level of EGFR in a cell or tissue, alone or in combination with other therapies. For example, a siNA molecule can comprise a delivery vehicle, including liposomes, for administration to a subject, carriers and diluents and their salts, and/or can be present in pharmaceutically acceptable formulations. Methods for the delivery of nucleic acid molecules are described in Akhtar et al., 1992, Trends Cell Bio., 2, 139; Delivery Strategies for Antisense Oligonucleotide Therapeutics, ed. Akbtar, 1995, Maurer et al., 1999, Mol. Membr. Biol., 16, 129-140; Hofland and Huang, 1999, Handb. Exp. Pharmacol., 137, 165-192; and Lee et al., 2000, ACS Symp. Ser., 752, 184-192, all of which are incorporated herein by reference. Beigelman et al., U.S. Pat. No. 6,395,713 and Sullivan et al., PCT WO 94/02595 further describe the general methods for delivery of nucleic acid molecules. These protocols can be utilized for the delivery of virtually any nucleic acid molecule. Nucleic acid molecules can be administered to cells by a variety of methods known to those of skill in the art, including, but not restricted to, encapsulation in liposomes, by iontophoresis, or by incorporation into other vehicles, such as hydrogels, cyclodextrins, biodegradable nanocapsules, and bioadhesive microspheres, or by proteinaceous vectors (O'Hare and Normand, International PCT Publication No. WO 00/53722). Alternatively, the nucleic acid/vehicle combination is locally delivered by direct injection or by use of an infusion pump. Direct injection of the nucleic acid molecules of the invention, whether subcutaneous, intramuscular, or intradermal, can take place using standard needle and syringe methodologies, or by needle-free technologies such as those described in Conry et al., 1999, Clin. Cancer Res., 5, 2330-2337 and Barry et al., International PCT Publication No. WO 99/31262. The molecules of the instant invention can be used as pharmaceutical agents. Pharmaceutical agents prevent, modulate the occurrence, or treat (alleviate a symptom to some extent, preferably all of the symptoms) of a disease state in a patient.

[0227] Thus, the invention features a pharmaceutical composition comprising one or more nucleic acid(s) of the invention in an acceptable carrier, such as a stabilizer, buffer, and the like. The polynucleotides of the invention can be administered (e.g., RNA, DNA or protein) and introduced into a patient by any standard means, with or without stabilizers, buffers, and the like, to form a pharmaceutical composition. When it is desired to use a liposome delivery mechanism, standard protocols for formation of liposomes can be followed. The compositions of the present invention can also be formulated and used as tablets, capsules or elixirs for oral administration, suppositories for rectal administration, sterile solutions, suspensions for injectable administration, and the other compositions known in the art.

[0228] The present invention also includes pharmaceutically acceptable formulations of the compounds described. These formulations include salts of the above compounds, e.g., acid addition salts, for example, salts of hydrochloric, hydrobromic, acetic acid, and benzene sulfonic acid.

[0229] A pharmacological composition or formulation refers to a composition or formulation in a form suitable for administration, e.g., systemic administration, into a cell or patient, including for example a human. Suitable forms, in part, depend upon the use or the route of entry, for example oral, transdermal, or by injection. Such forms should not prevent the composition or formulation from reaching a target cell (i.e., a cell to which the negatively charged nucleic acid is desirable for delivery). For example, pharmacological compositions injected into the blood stream should be soluble. Other factors are known in the art, and include considerations such as toxicity and forms that prevent the composition or formulation from exerting its effect.

[0230] By “systemic administration” is meant in vivo systemic absorption or accumulation of drugs in the blood stream followed by distribution throughout the entire body. Administration routes that lead to systemic absorption include, without limitation: intravenous, subcutaneous, intraperitoneal, inhalation, oral, intrapulmonary and intramuscular. Each of these administration routes exposes the siNA molecules of the invention to an accessible diseased tissue. The rate of entry of a drug into the circulation has been shown to be a function of molecular weight or size. The use of a liposome or other drug carrier comprising the compounds of the instant invention can potentially localize the drug, for example, in certain tissue types, such as the tissues of the reticular endothelial system (RES). A liposome formulation that can facilitate the association of drug with the surface of cells, such as, lymphocytes and macrophages is also useful. This approach can provide enhanced delivery of the drug to target cells by taking advantage of the specificity of macrophage and lymphocyte immune recognition of abnormal cells, such as cancer cells.

[0231] By “pharmaceutically acceptable formulation” is meant, a composition or formulation that allows for the effective distribution of the nucleic acid molecules of the instant invention in the physical location most suitable for their desired activity. Nonlimiting examples of agents suitable for formulation with the nucleic acid molecules of the instant invention include: P-glycoprotein inhibitors (such as Pluronic P85), which can enhance entry of drugs into the CNS (Jolliet-Riant and Tillement, 1999, Fundam. Clin. Pharmacol., 13, 16-26); biodegradable polymers, such as poly (DL-lactide-coglycolide) microspheres for sustained release delivery after intracerebral implantation (Emerich, DF et al, 1999, Cell Transplant, 8, 47-58) (Alkermes, Inc. Cambridge, Mass.); and loaded nanoparticles, such as those made of polybutylcyanoacrylate, which can deliver drugs across the blood brain barrier and can alter neuronal uptake mechanisms (Prog Neuropsychopharmacol Biol Psychiatry, 23, 941-949, 1999). Other non-limiting examples of delivery strategies for the nucleic acid molecules of the instant invention include material described in Boado et al., 1998, J. Pharm. Sci., 87, 1308-1315; Tyler et al., 1999, FEBS Lett., 421, 280-284; Pardridge et al., 1995, PNAS USA., 92, 5592-5596; Boado, 1995, Adv. Drug Delivery Rev., 15, 73-107; Aldrian-Herrada et al., 1998, Nucleic Acids Res., 26, 4910-4916; and Tyler et al., 1999, PNAS USA., 96, 7053-7058.

[0232] The invention also features the use of the composition comprising surface-modified liposomes containing poly (ethylene glycol) lipids (PEG-modified, or long-circulating liposomes or stealth liposomes). These formulations offer a method for increasing the accumulation of drugs in target tissues. This class of drug carriers resists opsonization and elimination by the mononuclear phagocytic system (MPS or RES), thereby enabling longer blood circulation times and enhanced tissue exposure for the encapsulated drug (Lasic et al. Chem. Rev. 1995, 95, 2601-2627; Ishiwata et al., Chem. Pharm. Bull. 1995, 43, 1005-1011). Such liposomes have been shown to accumulate selectively in tumors, presumably by extravasation and capture in the neovascularized target tissues (Lasic et al., Science 1995, 267, 1275-1276; Oku et al.,1995, Biochim. Biophys. Acta, 1238, 86-90). The long-circulating liposomes enhance the pharmacokinetics and pharmacodynamics of DNA and RNA, particularly compared to conventional cationic liposomes which are known to accumulate in tissues of the MPS (Liu et al., J. Biol. Chem. 1995, 42, 24864-24870; Choi et al., International PCT Publication No. WO 96/10391; Ansell et al., International PCT Publication No. WO 96/10390; Holland et al., International PCT Publication No. WO 96/10392). Long-circulating liposomes are also likely to protect drugs from nuclease degradation to a greater extent compared to cationic liposomes, based on their ability to avoid accumulation in metabolically aggressive MPS tissues such as the liver and spleen.

[0233] The present invention also includes compositions prepared for storage or administration, which include a pharmaceutically effective amount of the desired compounds in a pharmaceutically acceptable carrier or diluent. Acceptable carriers or diluents for therapeutic use are well known in the pharmaceutical art, and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co. (A. R. Gennaro edit. 1985) hereby incorporated by reference herein. For example, preservatives, stabilizers, dyes and flavoring agents can be provided. These include sodium benzoate, sorbic acid and esters of p-hydroxybenzoic acid. In addition, antioxidants and suspending agents can be used.

[0234] The present invention also includes compositions prepared for storage or administration that include a pharmaceutically effective amount of the desired compounds in a pharmaceutically acceptable carrier or diluent. Acceptable carriers or diluents for therapeutic use are well known in the pharmaceutical art, and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co. (A. R. Gennaro edit. 1985), hereby incorporated by reference herein. For example, preservatives, stabilizers, dyes and flavoring agents can be provided. These include sodium benzoate, sorbic acid and esters of p-hydroxybenzoic acid. In addition, antioxidants and suspending agents can be used.

[0235] A pharmaceutically effective dose is that dose required to prevent, inhibit the occurrence, or treat (alleviate a symptom to some extent, preferably all of the symptoms) of a disease state. The pharmaceutically effective dose depends on the type of disease, the composition used, the route of administration, the type of mammal being treated, the physical characteristics of the specific mammal under consideration, concurrent medication, and other factors that those skilled in the medical arts will recognize. Generally, an amount between 0.1 mg/kg and 100 mg/kg body weight/day of active ingredients is administered dependent upon potency of the negatively charged polymer.

[0236] The nucleic acid molecules of the invention and formulations thereof can be administered orally, topically, parenterally, by inhalation or spray, or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and/or vehicles. The term parenteral as used herein includes percutaneous, subcutaneous, intravascular (e.g., intravenous), intramuscular, or intrathecal injection or infusion techniques and the like. In addition, there is provided a pharmaceutical formulation comprising a nucleic acid molecule of the invention and a pharmaceutically acceptable carrier. One or more nucleic acid molecules of the invention can be present in association with one or more non-toxic pharmaceutically acceptable carriers and/or diluents and/or adjuvants, and if desired other active ingredients. The pharmaceutical compositions containing nucleic acid molecules of the invention can be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs.

[0237] Compositions intended for oral use can be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions can contain one or more such sweetening agents, flavoring agents, coloring agents or preservative agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients that are suitable for the manufacture of tablets. These excipients can be, for example, inert diluents; such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia; and lubricating agents, for example magnesium stearate, stearic acid or talc. The tablets can be uncoated or they can be coated by known techniques. In some cases such coatings can be prepared by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monosterate or glyceryl distearate can be employed.

[0238] Formulations for oral use can also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.

[0239] Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydropropyl-methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents can be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions can also contain one or more preservatives, for example ethyl, or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.

[0240] Oily suspensions can be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin. The oily suspensions can contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents and flavoring agents can be added to provide palatable oral preparations. These compositions can be preserved by the addition of an anti-oxidant such as ascorbic acid.

[0241] Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents or suspending agents are exemplified by those already mentioned above. Additional excipients, for example sweetening, flavoring and coloring agents, can also be present.

[0242] Pharmaceutical compositions of the invention can also be in the form of oil-in-water emulsions. The oily phase can be a vegetable oil or a mineral oil or mixtures of these. Suitable emulsifying agents can be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monooleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate. The emulsions can also contain sweetening and flavoring agents.

[0243] Syrups and elixirs can be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol, glucose or sucrose. Such formulations can also contain a demulcent, a preservative and flavoring and coloring agents. The pharmaceutical compositions can be in the form of a sterile injectable aqueous or oleaginous suspension. This suspension can be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents that have been mentioned above. The sterile injectable preparation can also be a sterile injectable solution or suspension in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil can be employed including synthetic mono-or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

[0244] The nucleic acid molecules of the invention can also be administered in the form of suppositories, e.g., for rectal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non-irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Such materials include cocoa butter and polyethylene glycols.

[0245] Nucleic acid molecules of the invention can be administered parenterally in a sterile medium. The drug, depending on the vehicle and concentration used, can either be suspended or dissolved in the vehicle. Advantageously, adjuvants such as local anesthetics, preservatives and buffering agents can be dissolved in the vehicle.

[0246] Dosage levels of the order of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions (about 0.5 mg to about 7 g per patient per day). The amount of active ingredient that can be combined with the carrier materials to produce a single dosage form varies depending upon the host treated and the particular mode of administration. Dosage unit forms generally contain between from about 1 mg to about 500 mg of an active ingredient.

[0247] It is understood that the specific dose level for any particular patient depends upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy.

[0248] For administration to non-human animals, the composition can also be added to the animal feed or drinking water. It can be convenient to formulate the animal feed and drinking water compositions so that the animal takes in a therapeutically appropriate quantity of the composition along with its diet. It can also be convenient to present the composition as a premix for addition to the feed or drinking water.

[0249] The nucleic acid molecules of the present invention can also be administered to a patient in combination with other therapeutic compounds to increase the overall therapeutic effect. The use of multiple compounds to treat an indication can increase the beneficial effects while reducing the presence of side effects.

[0250] In one embodiment, the invention comprises compositions suitable for administering nucleic acid molecules of the invention to specific cell types, such as hepatocytes. For example, the asialoglycoprotein receptor (ASGPr) (Wu and Wu, 1987, J. Biol. Chem. 262, 4429-4432) is unique to hepatocytes and binds branched galactose-terminal glycoproteins, such as asialoorosomucoid (ASOR). Binding of such glycoproteins or synthetic glycoconjugates to the receptor takes place with an affinity that strongly depends on the degree of branching of the oligosaccharide chain, for example, triatennary structures are bound with greater affinity than biatenarry or monoatennary chains (Baenziger and Fiete, 1980, Cell, 22, 611-620; Connolly et al., 1982, J. Biol. Chem., 257, 939-945). Lee and Lee, 1987, Glycoconjugate J., 4, 317-328, obtained this high specificity through the use of N-acetyl-D-galactosamine as the carbohydrate moiety, which has higher affinity for the receptor, compared to galactose. This “clustering effect” has also been described for the binding and uptake of mannosyl-terminating glycoproteins or glycoconjugates (Ponpipom et al., 1981, J. Med. Chem., 24, 1388-1395). The use of galactose and galactosamine based conjugates to transport exogenous compounds across cell membranes can provide a targeted delivery approach to the treatment of liver disease such as HBV infection or hepatocellular carcinoma. The use of bioconjugates can also provide a reduction in the required dose of therapeutic compounds required for treatment. Furthermore, therapeutic bioavialability, pharmacodynamics, and pharmacokinetic parameters can be modulated through the use of nucleic acid bioconjugates of the invention.

[0251] Alternatively, certain siNA molecules of the instant invention can be expressed within cells from eukaryotic promoters (e.g., Izant and Weintraub, 1985, Science, 229, 345; McGarry and Lindquist, 1986, Proc. Natl. Acad. Sci., USA 83, 399; Scanlon et al., 1991, Proc. Natl. Acad. Sci. USA, 88, 10591-5; Kashani-Sabet et al., 1992, Antisense Res. Dev., 2, 3-15; Dropulic et al., 1992, J. Virol., 66, 1432-41; Weerasinghe et al., 1991, J. Virol., 65, 5531-4; Ojwang et al., 1992, Proc. Natl. Acad. Sci. USA, 89, 10802-6; Chen et al., 1992, Nucleic Acids Res., 20, 4581-9; Sarver et al., 1990 Science, 247, 1222-1225; Thompson et al., 1995, Nucleic Acids Res., 23, 2259; Good et al., 1997, Gene Therapy, 4, 45. Those skilled in the art realize that any nucleic acid can be expressed in eukaryotic cells from the appropriate DNA/RNA vector. The activity of such nucleic acids can be augmented by their release from the primary transcript by a enzymatic nucleic acid (Draper et al., PCT WO 93/23569, and Sullivan et al., PCT WO 94/02595; Ohkawa et al., 1992, Nucleic Acids Symp. Ser., 27, 15-6; Taira et al., 1991, Nucleic Acids Res., 19, 5125-30; Ventura et al., 1993, Nucleic Acids Res., 21, 3249-55; Chowrira et al., 1994, J. Biol. Chem., 269, 25856.

[0252] In another aspect of the invention, RNA molecules of the present invention can be expressed from transcription units (see for example Couture et al., 1996, TIG., 12, 510) inserted into DNA or RNA vectors. The recombinant vectors can be DNA plasmids or viral vectors. siNA expressing viral vectors can be constructed based on, but not limited to, adeno-associated virus, retrovirus, adenovirus, or alphavirus. In another embodiment, pol III based constructs are used to express nucleic acid molecules of the invention (see for example Thompson, U.S. Pat. Nos. 5,902,880 and 6,146,886). The recombinant vectors capable of expressing the siNA molecules can be delivered as described above, and persist in target cells. Alternatively, viral vectors can be used that provide for transient expression of nucleic acid molecules. Such vectors can be repeatedly administered as necessary. Once expressed, the siNA molecule interacts with the target mRNA and generates an RNAi response. Delivery of siNA molecule expressing vectors can be systemic, such as by intravenous or intra-muscular administration, by administration to target cells ex-planted from a patient followed by reintroduction into the patient, or by any other means that would allow for introduction into the desired target cell (for a review see Couture et al., 1996, TIG., 12, 510).

[0253] In one aspect the invention features an expression vector comprising a nucleic acid sequence encoding at least one siNA molecule of the instant invention. The expression vector can encode one or both strands of a siNA duplex, or a single self-complementary strand that self hybridizes into a siNA duplex. The nucleic acid sequences encoding the siNA molecules of the instant invention can be operably linked in a manner that allows expression of the siNA molecule (see for example Paul et al., 2002, Nature Biotechnology, 19, 505; Miyagishi and Taira, 2002, Nature Biotechnology, 19, 497; Lee et al., 2002, Nature Biotechnology, 19, 500; and Novina et al., 2002, Nature Medicine, advance online publication doi:10.1038/nm725).

[0254] In another aspect, the invention features an expression vector comprising: a) a transcription initiation region (e.g., eukaryotic pol I, II or III initiation region); b) a transcription termination region (e.g., eukaryotic pol I, II or III termination region); and c) a nucleic acid sequence encoding at least one of the siNA molecules of the instant invention; wherein said sequence is operably linked to said initiation region and said termination region, in a manner that allows expression and/or delivery of the siNA molecule. The vector can optionally include an open reading frame (ORF) for a protein operably linked on the 5′ side or the 3′-side of the sequence encoding the siNA of the invention; and/or an intron (intervening sequences).

[0255] Transcription of the siNA molecule sequences can be driven from a promoter for eukaryotic RNA polymerase I (pol I), RNA polymerase II (pol II), or RNA polymerase III (pol III). Transcripts from pol II or pol III promoters are expressed at high levels in all cells; the levels of a given pol II promoter in a given cell type depends on the nature of the gene regulatory sequences (enhancers, silencers, etc.) present nearby. Prokaryotic RNA polymerase promoters are also used, providing that the prokaryotic RNA polymerase enzyme is expressed in the appropriate cells (Elroy-Stein and Moss, 1990, Proc. Natl. Acad. Sci. U S A, 87, 6743-7; Gao and Huang 1993, Nucleic Acids Res., 21, 2867-72; Lieber et al., 1993, Methods Enzymol., 217, 47-66; Zhou et al., 1990, Mol. Cell. Biol., 10, 4529-37). Several investigators have demonstrated that nucleic acid molecules expressed from such promoters can function in mammalian cells (e.g. Kashani-Sabet et al., 1992, Antisense Res. Dev., 2, 3-15; Ojwang et al., 1992, Proc. Natl. Acad. Sci. USA, 89, 10802-6; Chen et al., 1992, Nucleic Acids Res., 20, 4581-9; Yu et al., 1993, Proc. Natl. Acad. Sci. U S A, 90, 6340-4; L'Huillier et al., 1992, EMBO J, 11, 4411-8; Lisziewicz et al., 1993, Proc. Natl. Acad. Sci. U. S. A, 90, 8000-4; Thompson et al., 1995, Nucleic Acids Res., 23, 2259; Sullenger & Cech, 1993, Science, 262, 1566). More specifically, transcription units such as the ones derived from genes encoding U6 small nuclear (snRNA), transfer RNA (tRNA) and adenovirus VA RNA are useful in generating high concentrations of desired RNA molecules such as siNA in cells (Thompson et al., supra; Couture and Stinchcomb, 1996, supra; Noonberg et al., 1994, Nucleic Acid Res., 22, 2830; Noonberg et al., U.S. Pat. No. 5,624,803; Good et al., 1997, Gene Ther., 4, 45; Beigelman et al., International PCT Publication No. WO 96/18736. The above siNA transcription units can be incorporated into a variety of vectors for introduction into mammalian cells, including but not restricted to, plasmid DNA vectors, viral DNA vectors (such as adenovirus or adeno-associated virus vectors), or viral RNA vectors (such as retroviral or alphavirus vectors) (for a review see Couture and Stinchcomb, 1996, supra).

[0256] In another aspect the invention features an expression vector comprising a nucleic acid sequence encoding at least one of the siNA molecules of the invention, in a manner that allows expression of that siNA molecule. The expression vector comprises in one embodiment; a) a transcription initiation region; b) a transcription termination region; and c) a nucleic acid sequence encoding at least one strand of the siNA molecule; wherein the sequence is operably linked to the initiation region and the termination region, in a manner that allows expression and/or delivery of the siNA molecule.

[0257] In another embodiment the expression vector comprises: a) a transcription initiation region; b) a transcription termination region; c) an open reading frame; and d) a nucleic acid sequence encoding at least one strand of a siNA molecule, wherein the sequence is operably linked to the 3′-end of the open reading frame; and wherein the sequence is operably linked to the initiation region, the open reading frame and the termination region, in a manner that allows expression and/or delivery of the siNA molecule. In yet another embodiment the expression vector comprises: a) a transcription initiation region; b) a transcription termination region; c) an intron; and d) a nucleic acid sequence encoding at least one siNA molecule; wherein the sequence is operably linked to the initiation region, the intron and the termination region, in a manner which allows expression and/or delivery of the nucleic acid molecule.

[0258] In another embodiment, the expression vector comprises: a) a transcription initiation region; b) a transcription termination region; c) an intron; d) an open reading frame; and e) a nucleic acid sequence encoding at least one strand of a siNA molecule, wherein the sequence is operably linked to the 3′-end of the open reading frame; and wherein the sequence is operably linked to the initiation region, the intron, the open reading frame and the termination region, in a manner which allows expression and/or delivery of the siNA molecule.

EXAMPLES

[0259] The following are non-limiting examples showing the selection, isolation, synthesis and activity of nucleic acids of the instant invention.

Example 1 Tandem Synthesis of siNA Constructs

[0260] Exemplary siNA molecules of the invention are synthesized in tandem using a cleavable linker, for example a succinyl-based linker. Tandem synthesis as described herein is followed by a one-step purification process that provides RNAi molecules in high yield. This approach is highly amenable to siNA synthesis in support of high throughput RNAi screening, and can be readily adapted to multi-column or multi-well synthesis platforms.

[0261] After completing a tandem synthesis of an siNA oligo and its compliment in which the 5′-terminal dimethoxytrityl (5′-O-DMT) group remains intact (trityl on synthesis), the oligonucleotides are deprotected as described above. Following deprotection, the siNA sequence strands are allowed to spontaneously hybridize. This hybridization yields a duplex in which one strand has retained the 5′-O-DMT group while the complementary strand comprises a terminal 5′-hydroxyl. The newly formed duplex to behaves as a single molecule during routine solid-phase extraction purification (Trityl-On purification) even though only one molecule has a dimethoxytrityl group. Because the strands form a stable duplex, this dimethoxytrityl group (or an equivalent group, such as other trityl groups or other hydrophobic moieties) is all that is required to purify the pair of oligos, for example by using a C18 cartridge.

[0262] Standard phosphoramidite synthesis chemistry is used up to point of introducing a tandem linker, such as an inverted deoxy abasic succinate or glyceryl succinate linker (see FIG. 1) or an equivalent cleavable linker. A non-limiting example of linker coupling conditions that can be used includes a hindered base such as diisopropylethylamine (DIPA) and/or DMAP in the presence of an activator reagent such as Bromotripyrrolidinophosphoniumhexaflurorophosphate (PyBrOP). After the linker is coupled, standard synthesis chemistry is utilized to complete synthesis of the second sequence leaving the terminal the 5′-O-DMT intact. Following synthesis, the resulting oligonucleotide is deprotected according to the procedures described herein and quenched with a suitable buffer, for example with 50 mM NaOAc or 1.5M NH₄H₂CO₃.

[0263] Purification of the siNA duplex can be readily accomplished using solid phase extraction, for example using a Waters C18 SepPak 1 g cartridge conditioned with 1 column volume (CV) of acetonitrile, 2 CV H2O, and 2 CV 50 mM NaOAc. The sample is loaded and then washed with 1 CV H2O or 50 mM NaOAc. Failure sequences are eluted with 1 CV 14% ACN (Aqueous with 50 mM NaOAc and 50 mM NaCl). The column is then washed, for example with 1 CV H2O followed by on-column detritylation, for example by passing 1 CV of 1% aqueous trifluoroacetic acid (TFA) over the column, then adding a second CV of 1% aqueous TFA to the column and allowing to stand for approx. 10 minutes. The remaining TFA solution is removed and the column washed with H2O followed by 1 CV 1M NaCl and additional H2O. The siNA duplex product is then eluted, for example using 1 CV 20% aqueous CAN.

[0264]FIG. 2 provides an example of MALDI-TOV mass spectrometry analysis of a purified siNA construct in which each peak corresponds to the calculated mass of an individual siNA strand of the siNA duplex. The same purified siNA provides three peaks when analyzed by capillary gel electrophoresis (CGE), one peak presumably corresponding to the duplex siNA, and two peaks presumably corresponding to the separate siNA sequence strands. Ion exchange HPLC analysis of the same siNA contract only shows a single peak. Testing of the purified siNA construct using a luciferase reporter assay described below demonstrated the same RNAi activity compared to siNA constructs generated from separately synthesized oligonucleotide sequence strands.

Example 2 Identification of Potential siNA Target Sites in any RNA Sequence

[0265] The sequence of an RNA target of interest, such as a viral or human mRNA transcript, is screened for target sites, for example by using a computer folding algorithm. In a non-limiting example, the sequence of a gene or RNA gene transcript derived from a database, such as Genbank, is used to generate siNA targets having complimentarity to the target. Such sequences can be obtained from a database, or can be determined experimentally as known in the art. Target sites that are known, for example, those target sites determined to be effective target sites based on studies with other nucleic acid molecules, for example ribozymes or antisense, or those targets known to be associated with a disease or condition such as those sites containing mutations or deletions, can be used to design siNA molecules targeting those sites as well. Various parameters can be used to determine which sites are the most suitable target sites within the target RNA sequence. These parameters include but are not limited to secondary or tertiary RNA structure, the nucleotide base composition of the target sequence, the degree of homology between various regions of the target sequence, or the relative position of the target sequence within the RNA transcript. Based on these determinations, any number of target sites within the RNA transcript can be chosen to screen siNA molecules for efficacy, for example by using in vitro RNA cleavage assays, cell culture, or animal models. In a non-limiting example, anywhere from 1 to 1000 target sites are chosen within the transcript based on the size of the siNA contruct to be used. High throughput screening assays can be developed for screening siNA molecules using methods known in the art, such as with multi-well or multi-plate assays to determine efficient reduction in target gene expression.

Example 3 Selection of siNA Molecule Target Sites in a RNA

[0266] The following non-limiting steps can be used to carry out the selection of siNAs targeting a given gene sequence or transcipt.

[0267] 1. The target sequence is parsed in silico into a list of all fragments or subsequences of a particular length, for example 23 nucleotide fragments, contained within the target sequence. This step is typically carried out using a custom Perl script, but commercial sequence analysis programs such as Oligo, MacVector, or the GCG Wisconsin Package can be employed as well.

[0268] 2. In some instances the siNAs correspond to more than one target sequence; such would be the case for example in targeting many different strains of a viral sequence, for targeting different transcipts of the same gene, targeting different transcipts of more than one gene, or for targeting both the human gene and an animal homolog. In this case, a subsequence list of a particular length is generated for each of the targets, and then the lists are compared to find matching sequences in each list. The subsequences are then ranked according to the number of target sequences that contain the given subsequence; the goal is to find subsequences that are present in most or all of the target sequences. Alternately, the ranking can indentify subsequences that are unique to a target sequence, such as a mutant target sequence. Such an approach would enable the use of siNA to target specifically the mutant sequence and not effect the expression of the normal sequence.

[0269] 3. In some instances the siNA subsequences are absent in one or more sequences while present in the desired target sequence; such would be the case if the siNA targets a gene with a paralogous family member that is to remain untargeted. As in case 2 above, a subsequence list of a particular length is generated for each of the targets, and then the lists are compared to find sequences that are present in the target gene but are absent in the untargeted paralog.

[0270] 4. The ranked siNA subsequences can be further analyzed and ranked according to GC content. A preference can be given to sites containing 30-70% GC, with a further preference to sites containing 40-60% GC.

[0271] 5. The ranked siNA subsequences can be further analyzed and ranked according to self-folding and internal hairpins. Weaker internal folds are preferred; strong hairpin structures are to be avoided.

[0272] 6. The ranked siNA subsequences can be further analyzed and ranked according to whether they have runs of GGG or CCC in the sequence. GGG (or even more Gs) in either strand can make oligonucleotide synthesis problematic, so it is avoided whenever better sequences are available. CCC is searched in the target strand because that will place GGG in the antisense strand.

[0273] 7. The ranked siNA subsequences can be further analyzed and ranked according to whether they have the dinucleotide UU (uridine dinucleotide) on the 3′ end of the sequence, and/or AA on the 5′ end of the sequence (to yield 3′ UU on the antisense sequence). These sequences allow one to design siNA molecules with terminal TT thymidine dinucleotides.

[0274] 8. Four or five target sites are chosen from the ranked list of subsequences as described above. For example, in subsequences having 23 nucleotides, the right 21 nucleotides of each chosen 23-mer subsequence are then designed and synthesized for the upper (sense) strand of the siNA duplex, while the reverse complement of the left 21 nucleotides of each chosen 23-mer subsequence are then designed and synthesized for the lower (antisense) strand of the siNA duplex. If terminal TT residues are desired for the sequence (as described in paragraph 7), then the two 3′ terminal nucleotides of both the sense and antisense strands are replaced by TT prior to synthesizing the oligos.

[0275] 9. The siNA molecules are screened in an in vitro, cell culture or animal model system to identify the most active siNA molecule or the most preferred target site within the target RNA sequence.

[0276] In an alternate approach, a pool of siNA constructs specific to an EGFR (e.g., HER1, HER2) target sequence is used to screen for target sites in cells expressing EGFR RNA. The general strategy used in this approach is shown in FIG. 18. Cells expressing EGFR (e.g., HER1, HER2) are transfected with the pool of siNA constructs and cells that demonstrate a phenotype associated with EGFR (e.g., HER1, HER2) inhibition are sorted. The pool of siNA constructs can be expressed from transcription cassettes inserted into appropriate vectors (see for example FIG. 16 and FIG. 17). Cells in which EGFR (e.g., HER1, HER2) expression is decreased due to siNA treatment demonstrate a phenotypic change, for example decreased production of EGFR (e.g., HER1, HER2) RNA or protein(s) compared to untreated cells or cells treated with a control siNA. The siNA from cells demonstrating a positive phenotypic change (e.g., decreased EGFR EGFR (e.g., HER1, HER2) RNA or protein), are sequenced to determine the most suitable target site(s) within the target RNA sequence.

Example 4 EGFR Targeted siNA Design

[0277] siNA target sites were chosen by analyzing sequences of the EGFR (e.g., HER1, HER2) RNA target and optionally prioritizing the target sites on the basis of folding (structure of any given sequence analyzed to determine siNA accessibility to the target). siNA molecules were designed that could bind each target and are optionally individually analyzed by computer folding to assess whether the siNA molecule can interact with the target sequence. Varying the length of the siNA molecules can be chosen to optimize activity. Generally, a sufficient number of complementary nucleotide bases are chosen to bind to, or otherwise interact with, the target RNA, but the degree of complementarity can be modulated to accommodate siNA duplexes or varying length or base composition. By using such methodologies, siNA molecules can be designed to target sites within any known RNA sequence, for example those RNA sequences corresponding to the any gene transcript.

Example 5 Chemical Synthesis and Purification of siNA

[0278] siNA molecules can be designed to interact with various sites in the RNA message, for example target sequences within the RNA sequences described herein. The sequence of one strand of the siNA molecule(s) are complementary to the target site sequences described above. The siNA molecules can be chemically synthesized using methods described herein. Inactive siNA molecules that are used as control sequences can be synthesized by scrambling the sequence of the siNA molecules such that it is not complementary to the target sequence.

Example 6 In Vivo Models Used to Evaluate the Down-Regulation of EGFR Gene Expression

[0279] Nucleic acid molecules targeted to the human EGFR RNA are designed and synthesized as described above. These nucleic acid molecules can be tested for cleavage activity in vivo, for example, using the procedures described below. A variety of endpoints have been used in cell culture models to evaluate EGFR-mediated effects after treatment with anti-EGFR agents. Phenotypic endpoints include inhibition of cell proliferation, apoptosis assays and reduction of EGFR protein expression. Because overexpression of EGFR is directly associated with increased proliferation of tumor cells, a proliferation endpoint for cell culture assays is preferably used as a primary screen. There are several methods by which this endpoint can be measured. Following treatment of cells with nucleic acid molecules, cells are allowed to grow (typically 5 days) after which either the cell viability, the incorporation of [³H] thymidine into cellular DNA and/or the cell density can be measured. The assay of cell density is well-known to those skilled in the art and can, for example, be performed in a 96-well format using commercially available fluorescent nucleic acid stains (such as Syto® 13 or CyQuant®) or the ability of live cells to reduce MTS to formazon (Promega, Madison, Wis.). For example, the MTS assay is described herein.

[0280] As a secondary, confirmatory endpoint, a nucleic acid-mediated decrease in the level of EGFR RNA and/or EGFR protein expression can be evaluated using methods known in the art, such as RT-PCR, Northern blot, ELISA, Western blot, and immunoprecipitation analyses, to name a few techniques.

[0281] Validation of Cell Lines and Ribozyme Treatment Conditions

[0282] Two human cell lines (SKBR-3 and SKOV-3) that are known to express medium to high levels of EGFR protein are considered for nucleic acid screening. In order to validate these cell lines for EGFR-mediated sensitivity, both cell lines are treated with an EGFR specific antibody, for example mAB IMC-C225 (ImClone) and its effect on cell proliferation is determined. mAB is added to cells at concentrations ranging from 0-8 μM in medium containing either no serum (OptiMem), 0.1% or 0.5% FBS and efficacy is determined via cell proliferation. Inhibition of proliferation (˜50%) in both cell lines after addition of mAB at 0.5 nM in medium containing 0.1% or no FBS, indicates that both cell lines are sensitive to an anti-EGFR agent (mAB) and supports their use in experiments testing anti-EGFR nucleic acid molecules.

[0283] Prior to nucleic acid screening, the choice of the optimal lipid(s) and conditions for nucleic acid delivery is determined empirically for each cell line. Applicant has established a panel of cationic lipids (lipids as described in PCT application WO99/05094) that can be used to deliver nucleic acids to cultured cells and are useful for cell proliferation assays that are typically 3-5 days in length. Additional description of useful lipids is provided above, and those skilled in the art are also familiar with a variety of lipids that can be used for delivery of oligonucleotide to cells in culture. Initially, this panel of lipid delivery vehicles is screened in SKBR-3 and SKOV-3 cells using previously established control oligonucleotides. Specific lipids and conditions for optimal delivery are selected for each cell line based on these screens. These conditions are used to deliver EGFR specific nucleic acids to cells for primary (inhibition of cell proliferation) and secondary (decrease in EGFR RNA/protein) efficacy endpoints.

[0284] Primary Screen: Inhibition of Cell Proliferation

[0285] Nucleic acid screens were performed using an automated, high throughput 96-well cell proliferation assay. Cell proliferation was measured over a 5-day treatment period using the MTS assay for determining cell density. The growth of cells treated with siNA/lipid complexes was compared to untreated cells, lipid treatment alone, and to cells treated with a inverted control sequence. Inverted controls can no longer bind to the target site due to a reversal of the native sequence. These controls are used to determine non-specific inhibition of cell growth caused by nucleic acid chemistry. The growth of cells treated with siNA/lipid complexes was compared to untreated cells, lipid treatment alone, and to cells treated with an inverted control sequence. Lead nucleic acids are chosen from the primary screen based on their ability to inhibit cell proliferation in a specific manner. Dose response assays are carried out on these leads and a subset are advanced into a secondary screen using a reduction in the level of EGFR protein and/or RNA as an endpoint.

[0286] Secondary Screen: Decrease in EGFR Protein and/or RNA

[0287] A secondary screen that measures the effect of anti-EGFR nucleic acids on EGFR protein and/or RNA levels is used to affirm preliminary findings. A EGFR ELISA for both SKBR-3 and SKOV-3 cells can been established and made available for use as an additional endpoint. In addition, a real time RT-PCR assay (TaqMan assay) has been developed to assess EGFR RNA reduction. Dose response activity of nucleic acid molecules of the instant invention can be used to assess both EGFR protein and RNA reduction endpoints.

[0288] siNA Mechanism Assays

[0289] A TaqMan® assay for measuring the siNA-mediated decrease in EGFR RNA has been established. This assay is based on PCR technology and can measure in real time the production of EGFR mRNA relative to a standard cellular mRNA such as 36B4. This RNA assay is used to establish proof that lead siNAs are working through an RNA cleavage mechanism and result in a decrease in the level of EGFR mRNA, thus leading to a decrease in cell surface EGFR protein receptors and a subsequent decrease in tumor cell proliferation.

[0290] Animal Models

[0291] Evaluating the efficacy of anti-HER2 agents in animal models is an important prerequisite to human clinical trials. As in cell culture models, the most HER2 sensitive mouse tumor xenografts are those derived from human carcinoma cells that express high levels of HER2 protein. In a recent study, nude mice bearing human vulvar (A431), lung (A549 and SK-LC-16 NSCL and LX-1) and prostate (PC-3 and TSU-PRI) xenografts were sensitive to the anti-HER2 tyrosine kinase inhibitor ZD1839 (Iressa), resulting in a partial regression of A431 tumor growth, 70-80% inhibition of tumor growth (A549, SKLC-16, TSU-PRI and PC-3 tumors), and 50-55% inhibition against the LX-1 tumor at a 150 mg kg dose (ip, every 3-4 days×4), (Sirotnak et al., 2000, Clin. Cancer Res., 6, 4885-48892). This same study compared the efficacy of ZD1839 alone or in combination with the commonly used chemotherapeutics, cisplatin, carboplatin, paclitaxel, docetaxel, edatrexate, gemcitabine, vinorelbine. When used in combination with certain chemotherapeutic agents, most notably cisplatin, carboplatin, paclitaxel, docetaxel, and edatrexate, marked response was observed compared to treatment with these agents alone, resulting in partial or complete regression in some cases. The above studies provide evidence that inhibition of HER2 expression by anti-HER2 agents causes inhibition of tumor growth in animals.

[0292] Animal Model Development

[0293] Tumor cell lines (SKBR-3 and SKOV-3) are characterized to establish their growth curves in mice. These cell lines are implanted into both nude and SCID mice and primary tumor volumes are measured 3 times per week. Growth characteristics of these tumor lines using a Matrigel implantation format can also be established. The use of other cell lines that have been engineered to express high levels of EGFR can also be used in the described studies. The tumor cell line(s) and implantation method that supports the most consistent and reliable tumor growth is used in animal studies testing the lead EGFR nucleic acid(s). Nucleic acids are administered by daily subcutaneous injection or by continuous subcutaneous infusion from Alzet mini osmotic pumps beginning 3 days after tumor implantation and continuing for the duration of the study. Group sizes of at least 10 animals are employed. Efficacy is determined by statistical comparison of tumor volume of nucleic acid-treated animals to a control group of animals treated with saline alone. Because the growth of these tumors is generally slow (45-60 days), an initial endpoint is the time in days it takes to establish an easily measurable primary tumor (i.e. 50-100 mm³) in the presence or absence of nucleic acid treatment.

[0294] EGFR Protein Levels for Patient Screening and as a Potential Endpoint

[0295] Because elevated EGFR levels can be detected in several cancers, cancer patients can be pre-screened for elevated EGFR prior to admission to initial clinical trials testing an anti-EGFR nucleic acid. Initial EGFR levels can be determined (by ELISA) from tumor biopsies or resected tumor samples. During clinical trials, it may be possible to monitor circulating EGFR protein by ELISA. Evaluation of serial blood/serum samples over the course of the anti-EGFR nucleic acid treatment period could be useful in determining early indications of efficacy.

Example 7 RNAi Mediated Inhibition of HER2 Expression

[0296] Unmodified and chemically-modified (see Table II) siNAs against HER2 site 2344 were tested for the ability to reduce endogenous HER2 RNA and protein in the HER2 overexpressing breast cancer cell line SK-BR-3. Additionally, siNAs were tested for the ability to inhibit proliferation of SK-BR-3 cells. Further, unmodified and additional chemically-modified siNAs (see Table II) against HER2 site 2344 were tested for the ability to reduce endogenous HER2 RNA in the HER2 overexpressing ovarian cancer cell line SK-OV-3.

[0297] SK-BR-3 cells were maintained in McCoy's medium (GIBCO/BRL, Bethesda, Md.) supplemented with 10% fetal bovine serum, L-glutamine (2 mM), bovine insulin (10 μg/mL). SK-OV-3 cells were maintained in EMEM medium (GIBCO/BRL, Bethesda, Md.) supplemented with 10% fetal bovine serum.

[0298] Cells were seeded in 96-well plates at a density of 7,500 and 5,000 cells/well for SK-BR-3 and SK-OV-3 cells, respectivelyin 100 μL of growth medium and incubated at 37° C. under 5% CO₂ for 24 h. Transfection of siNAs or inverted controls for RNA and protein endpoints was achieved by the following method: a 5×mixture of siNA (1.95-250 nM) and a cationic lipid formulation (20 μg/mL) was made in 150 μL of growth medium. siNA/lipid complexes were allowed to form for 20 min at 37° C. under 5% CO₂. A 25 μL aliquot of 5×siNA/lipid complexes was then added to treatment wells containing 100 μL of medium, resulting in a 1×final concentration of siNA (0.39-50 nM) and lipid (4 μg/mL). siNA/lipid complexes were left on cells for 24 h (RNA endpoint) or 48 h (protein endpoint).

[0299] Total RNA was purified from transfected cells at 24 h post-treatment. Real time RT-PCR (Taqman assay) was performed on purified RNA samples using separate primer/probe sets for target HER2 mRNA or control 36B4 RNA. 36B4 RNA levels were used to normalize for differences in well to well sample recovery. RT-PCR conditions were: 30 min at 48° C., 10 min at 95° C., followed by 40 cycles of 15 sec at 95° C. and 1 min at 60° C. Reactions were performed on an ABI Prism 7700 sequence detector. Results for all RNA siNA constructs are shown in FIGS. 3 and 4, whereas results for chemically-modified siNA constructs compared to all RNA (unmodified) constructs are shown in FIGS. 8-10 as the average of triplicate treatments±SD.

[0300] HER2 protein levels were determined by ELISA 48 h post-treatment. HER2 protein levels were normalized to cell number (MTS assay) to control for differences in well to well sample recovery. Results are shown in FIGS. 6 and 7 as the average of duplicate treatments±SD.

[0301] Transfection of siNAs for proliferation assays was the same as above except for the following changes. Short pulse transfection and multiple dosing was used, at 24 h post-plating 5×siNA/lipid complexes were added to and left on cells for 4 h then removed and replaced with growth medium. Final concentration of siNA and inverted controls was 6.25-50 nM. A second dose of siNA/lipid was added at 72 h post-plating and once again replaced with growth medium after 4 h of treatment. Inhibition of cell growth was determined by MTS assay at 48, 72 and 96 h post-treatment. Data for the 96 h point is shown in FIG. 5. Results are shown as the average of triplicate treatments±SD. As shown in FIG. 5, significant inhibition of proliferation is observed using both all RNA and chemically-modified siNA constructs targeting HER2 site 2344 in SKBR-3 cells.

Example 8 RNAi In Vitro Assay to Assess siNA Activity

[0302] An in vitro assay that recapitulates RNAi in a cell free system is used to evaluate siNA constructs targeting EGFR (e.g., HER1, HER2) RNA targets. The assay comprises the system described by Tuschl et al, 1999, Genes and Development, 13, 3191-3197 and Zamore et al., 2000, Cell, 101, 25-33 adapted for use with HER2 target RNA. A Drosophila extract derived from syncytial blastoderm is used to reconstitute RNAi activity in vitro. Target RNA is generated via in vitro transcription from an appropriate EGFR (e.g., HER1, HER2) expressing plasmid using T7 RNA polymerase or via chemical synthesis as described herein. Sense and antisense siNA strands (for example 20 uM each) are annealed by incubation in buffer (such as 100 mM potassium acetate, 30 mM HEPES-KOH, pH 7.4, 2 mM magnesium acetate) for 1 min. at 90° C. followed by 1 hour at 37° C., then diluted in lysis buffer (for example 100 mM potassium acetate, 30 mM HEPES-KOH at pH 7.4, 2mM magnesium acetate). Annealing can be monitored by gel electrophoresis on an agarose gel in TBE buffer and stained with ethidium bromide. The Drosophila lysate is prepared using zero to two hour old embryos from Oregon R flies collected on yeasted molasses agar that are dechorionated and lysed. The lysate is centrifuged and the supernatant isolated. The assay comprises a reaction mixture containing 50% lysate [vol/vol], RNA (10-50 pM final concentration), and 10% [vol/vol] lysis buffer containing siNA (10 nM final concentration). The reaction mixture also contains 10 mM creatine phosphate, 10 ug.ml creatine phosphokinase, 100 um GTP, 100 uM UTP, 100 uM CTP, 500 uM ATP, 5 mM DTT, 0.1 U/uL RNasin (Promega), and 100 uM of each amino acid. The final concentration of potassium acetate is adjusted to 100 mM. The reactions are pre-assembled on ice and preincubated at 25° C. for 10 minutes before adding RNA, then incubated at 25° C. for an additional 60 minutes. Reactions are quenched with 4 volumes of 1.25×Passive Lysis Buffer (Promega). Target RNA cleavage is assayed by RT-PCR analysis or other methods known in the art and are compared to control reactions in which siNA is omitted from the reaction.

[0303] Alternately, internally-labeled target RNA for the assay is prepared by in vitro transcription in the presence of [a-³²p] CTP, passed over a G 50 Sephadex column by spin chromatography and used as target RNA without further purification. Optionally, target RNA is 5′-³²P-end labeled using T4 polynucleotide kinase enzyme. Assays are performed as described above and target RNA and the specific RNA cleavage products generated by RNAi are visualized on an autoradiograph of a gel. The percentage of cleavage is determined by Phosphor Imager® quantitation of bands representing intact control RNA or RNA from control reactions without siNA and the cleavage products generated by the assay.

[0304] In one embodiment, this assay is used to determine target sites the EGFR (e.g., HER1, HER2) RNA target for siNA mediated RNAi cleavage, wherein a plurality of siNA constructs are screened for RNAi mediated cleavage of the EGFR (e.g., HER1, HER2) RNA target, for example by analyzing the assay reaction by electrophoresis of labeled target RNA, or by northern blotting, as well as by other methodology well known in the art.

Example 9 Diagnostic Uses

[0305] The siNA molecules of the invention can be used in a variety of diagnostic applications, such as in identifying molecular targets such as RNA in a variety of applications, for example, in clinical, industrial, environmental, agricultural and/or research settings. Such diagnostic use of siNA molecules involves utilizing reconstituted RNAi systems, for example using cellular lysates or partially purified cellular lysates. siNA molecules of this invention can be used as diagnostic tools to examine genetic drift and mutations within diseased cells or to detect the presence of endogenous or exogenous, for example viral, RNA in a cell. The close relationship between siNA activity and the structure of the target RNA allows the detection of mutations in any region of the molecule, which alters the base-pairing and three-dimensional structure of the target RNA. By using multiple siNA molecules described in this invention, one can map nucleotide changes, which are important to RNA structure and function in vitro, as well as in cells and tissues. Cleavage of target RNAs with siNA molecules can be used to inhibit gene expression and define the role (essentially) of specified gene products in the progression of disease or infection. In this manner, other genetic targets can be defined as important mediators of the disease. These experiments will lead to better treatment of the disease progression by affording the possibility of combination therapies (e.g., multiple siNA molecules targeted to different genes, siNA molecules coupled with known small molecule inhibitors, or intermittent treatment with combinations siNA molecules and/or other chemical or biological molecules). Other in vitro uses of siNA molecules of this invention are well known in the art, and include detection of the presence of mRNAs associated with a disease, infection, or related condition. Such RNA is detected by determining the presence of a cleavage product after treatment with a siNA using standard methodologies, for example fluorescence resonance emission transfer (FRET).

[0306] In a specific example, siNA molecules that can cleave only wild-type or mutant forms of the target RNA are used for the assay. The first siNA molecules is used to identify wild-type RNA present in the sample and the second siNA molecules will be used to identify mutant RNA in the sample. As reaction controls, synthetic substrates of both wild-type and mutant RNA will be cleaved by both siNA molecules to demonstrate the relative siNA efficiencies in the reactions and the absence of cleavage of the “non-targeted” RNA species. The cleavage products from the synthetic substrates will also serve to generate size markers for the analysis of wild-type and mutant RNAs in the sample population. Thus each analysis will require two siNA molecules, two substrates and one unknown sample which will be combined into six reactions. The presence of cleavage products will be determined using an RNase protection assay so that full-length and cleavage fragments of each RNA can be analyzed in one lane of a polyacrylamide gel. It is not absolutely required to quantify the results to gain insight into the expression of mutant RNAs and putative risk of the desired phenotypic changes in target cells. The expression of mRNA whose protein product is implicated in the development of the phenotype (i.e., disease related or infection related) is adequate to establish risk. If probes of comparable specific activity are used for both transcripts, then a qualitative comparison of RNA levels will be adequate and will decrease the cost of the initial diagnosis. Higher mutant form to wild-type ratios will be correlated with higher risk whether RNA levels are compared qualitatively or quantitatively.

[0307] All patents and publications mentioned in the specification are indicative of the levels of skill of those skilled in the art to which the invention pertains. All references cited in this disclosure are incorporated by reference to the same extent as if each reference had been incorporated by reference in its entirety individually.

[0308] One skilled in the art would readily appreciate that the present invention is well adapted to carry out the objects and obtain the ends and advantages mentioned, as well as those inherent therein. The methods and compositions described herein as presently representative of preferred embodiments are exemplary and are not intended as limitations on the scope of the invention. Changes therein and other uses will occur to those skilled in the art, which are encompassed within the spirit of the invention, are defined by the scope of the claims.

[0309] It will be readily apparent to one skilled in the art that varying substitutions and modifications can be made to the invention disclosed herein without departing from the scope and spirit of the invention. Thus, such additional embodiments are within the scope of the present invention and the following claims.

[0310] The invention illustratively described herein suitably can be practiced in the absence of any element or elements, limitation or limitations that are not specifically disclosed herein. Thus, for example, in each instance herein any of the terms “comprising”, “consisting essentially of” and “consisting of” may be replaced with either of the other two terms. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention that in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments, optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the description and the appended claims.

[0311] In addition, where features or aspects of the invention are described in terms of Markush groups or other grouping of alternatives, those skilled in the art will recognize that the invention is also thereby described in terms of any individual member or subgroup of members of the Markush group or other group. TABLE 1 HER2 siNA and Target Sequences Seq Seq Seq Pos Target Sequence ID UPos Upper seq ID Lpos Lower seq ID 1 AAGGGGAGGUAACCCUGGC 1 1 AAGGGGAGGUAACCCUGGC 1 23 GCCAGGGUUACCUCCCCUU 250 19 CCCCUUUGGUCGGGGCCCC 2 19 CCCCUUUGGUCGGGGCCCC 2 41 GGGGCCCCGACCXAAGGGG 251 37 CGGGCAGCCGCGCGCCCCU 3 37 CGGGCAGCCGCGCGCCCCU 3 59 AGGGGCGCGCGGCUGCCCG 252 55 UUCCCACGGGGCCCUUUAC 4 55 UUCCCACGGGGCCCUUUAC 4 77 GUAAAGGGCCCCGUGGGAA 253 73 CUGCGCCGCGOGCCCGGCC 5 73 CUGCGCCGCGCGCCCGGCC 5 95 GGCCGGGCGCGCGGCGCAG 254 91 CCCCACCCCUCGCAGCACC 6 91 CCCCACCCCUCGCAGCACC 6 113 GGUGCUGCGAGGGGUGGGG 255 109 CCCGCGCCCCGCGCCCUCC 7 109 CCCGCGCCCCGCGCCCUCC 7 131 GGAGGGCGCGGGGCGCGGG 256 127 CCAGCCGGGUCCAGCCGGA 8 127 CCAGCCGGGUCCAGCCGGA 8 149 UCCGGCUGGACCCGGCUGG 257 145 AGCCAUGGGGCCGGAGCCG 9 145 AGCCAUGGGGCCGGAGCCG 9 167 CGGCUCCGGCCCCAUGGCU 258 163 GCAGUGAGCACCAUGGAGC 10 163 GCAGUGAGCACCAUGGAGC 10 185 GCUCCAUGGUGCUCACUGC 259 181 CUGGCGGCCUUGUGCCGCU 11 181 CUGGCGGCCUUGUGCCGCU 11 203 AGCGGCACAAGGCCGCCAG 260 199 UGGGGGCUCCUCCUCGCCC 12 199 UGGGGGCUCCUCCUCGCCC 12 221 GGGCGAGGAGGAGCCCCCA 261 217 CUCUUGCCCCCCGGAGCCG 13 217 CUCUUGCCCCCCGGAGCCG 13 239 CGGCUCCGGGGGGCAAGAG 262 235 GCGAGCACCCAAGUGUGCA 14 235 GCGAGCACCCAAGUGUGCA 14 257 UGCACACUUGGGUGCUCGC 263 253 ACCGGCACAGACAUGAAGC 15 253 ACCGGCACAGACAUGAAGC 15 275 GCUUCAUGUCUGUGCCGGU 264 271 CUGCGGCUCCCUGCCAGUC 16 271 CUGCGGCUCCCUGCCAGUC 16 293 GACUGGCAGGGAGCCGCAG 265 289 CCCGAGACCCACCUGGACA 17 289 CCCGAGACCCACCUGGACA 17 311 UGUCCAGGUGGGUCUCGGG 266 307 AUGCUCCGCCACCUCUACC 18 307 AUGCUCCGCCACCUCUACC 18 329 GGUAGAGGUGGCGGAGCAU 267 325 CAGGGCUGCCAGGUGGUGC 19 325 CAGGGCUGCCAGGUGGUGC 19 347 GCACCACCUGGCAGCCCUG 268 343 CAGGGAAACCUGGAACUCA 20 343 CAGGGCAACCUGGAACUCA 20 365 UGAGUUCCAGGUUUCCCUG 269 361 ACCUACCUGCCCACCAAUG 21 361 ACCUACCUGCCCACCAAUG 21 383 CAUUGGUGGGCAGGUAGGU 270 379 GCCAGCCUGUCCUUCCUGC 22 379 GCCAGCCUGUCCUUCCUGC 22 401 GCAGGAAGGACAGGCUGGC 271 397 CAGGAUAUCCAGGAGGUGC 23 397 CAGGAUAUCCAGGAGGUGC 23 419 GCACCUCCUGGAUAUCCUG 272 415 CAGGGCUACGUGCUCAUCG 24 415 CAGGGCUACGUGCUCAUCG 24 437 CGAUGAGCACGUAGCCCUG 273 433 GCUCACAACCAAGUGAGGC 25 433 GCUCACAACCAAGUGAGGC 25 455 GCCUCACUUGGUUGUGAGC 274 451 CAGGUCCCACUGCAGAGGC 26 451 CAGGUCCCACUGCAGAGGC 26 473 GCCUCUGCAGUGGGACCUG 275 469 CUGCGGAUUGUGCGAGGCA 27 469 CUGCGGAUUGUGCGAGGCA 27 491 UGCCUCGCACAAUCCGCAG 276 487 ACCCAGCUCUUUGAGGACA 28 487 ACCCAGCUCUUUGAGGACA 28 509 UGUCCUCAAAGAGCUGGGU 277 505 AACUAUGCCCUGGCCGUGC 29 505 AACUAUGCCCUGGCCGUGC 29 527 GCACGGCCAGGGCAUAGUU 278 523 CUAGACAAUGGAGACCCGC 30 523 CUAGACAAUGGAGACCCGC 30 545 GCGGGUCUCCAUUGUCUAG 279 541 CUGAACAAUACCACCCCUG 31 541 CUGAACAAUACCACCCCUG 31 563 CAGGGGUGGUAUUGUUCAG 280 559 GUCACAGGGGCCUCCCCAG 32 559 GUCACAGGGGCCUCCCCAG 32 581 CUGGGGAGGCCCCUGUGAC 281 577 GGAGGCCUGCGGGAGCUGC 33 577 GGAGGCCUGCGGGAGCUGC 33 599 GCAGCUCCCGCAGGCCUCC 282 595 CAGCUUCGAAGCCUCACAG 34 595 CAGCUUCGAAGCCUCACAG 34 617 CUGUGAGGCUUCGAAGCUG 283 613 GAGAUCUUGAAAGGAGGGG 35 613 GAGAUCUUGAAAGGAGGGG 35 635 CCCCUCCUUUCAAGAUCUC 284 631 GUCUUGAUCCAGCGGAACC 36 631 GUCUUGAUCCAGCGGAACC 36 653 GGUUCCGCUGGAUCAAGAC 285 649 CCCCAGCUCUGCUACCAGG 37 649 CCCCAGCUCUGCUACCAGG 37 671 CCUGGUAGCAGAGCUGGGG 286 667 GACACGAUUUUGUGGAAGG 38 667 GACACGAUUUUGUGGAAGG 38 689 CCUUCCACAAAAUCGUGUC 287 685 GACAUCUUCCACAAGAACA 39 685 GACAUCUUCCACAAGAACA 39 707 UGUUCUUGUGGAAGAUGUC 288 703 AACCAGCUGGCUCUCACAC 40 703 AACCAGCUGGCUCUCACAC 40 725 GUGUGAGAGCCAGCUGGUU 289 721 CUGAUAGACACCAACCGCU 41 721 CUGAUAGACACCAACCGCU 41 743 AGCGGUUGGUGUCUAUCAG 290 739 UCUCGGGCCUGCCACCCCU 42 739 UCUCGGGCCUGCCACCCCU 42 761 AGGGGUGGCAGGCCCGAGA 291 757 UGUUCUCCGAUGUGUAAGG 43 757 UGUUCUCCGAUGUGUAAGG 43 779 CCUUACACAUCGGAGAACA 292 775 GGCUCCCGCUGCUGGGGAG 44 775 GGCUCCCGCUGCUGGGGAG 44 797 CUCCCCAGCAGCGGGAGCC 293 793 GAGAGUUCUGAGGAUUGUC 45 793 GAGAGUUCUGAGGAUUGUC 45 815 GACAAUCCUCAGAACUCUC 294 811 CAGAGCCUGACGCGCACUG 46 811 CAGAGCCUGACGCGCACUG 46 833 CAGUGOGOGUCAGGOUCUG 295 829 GUCUGUGCCGGUGGCUGUG 47 829 GUCUGUGCCGGUGGCUGUG 47 851 CACAGCCACCGGCACAGAC 296 847 GCCCGCUGCAAGGGGCCAC 48 847 GCCCGCUGCAAGGGGCCAC 48 869 GUGGCCCCUUGCAGCGGGC 297 865 CUGCCCACUGACUGCUGCC 49 865 CUGCCCACUGACUGCUGCC 49 887 GGCAGCAGUCAGUGGGCAG 298 883 CAUGAGCAGUGUGCUGCCG 50 883 CAUGAGCAGUGUGCUGCCG 50 905 CGGCAGCACACUGCUCAUG 299 901 GGCUGCACGGGCCCCAAGC 51 901 GGCUGCACGGGCCCCAAGC 51 923 GCUUGGGGCCCGUGCAGCC 300 919 CACUCUGACUGCCUGGCCU 52 919 CACUCUGACUGCCUGGCCU 52 941 AGGCCAGGCAGUCAGAGUG 301 937 UGCCUCCACUUCAACCACA 53 937 UGCCUCCACUUCAACCACA 53 959 UGUGGUUGAAGUGGAGGCA 302 955 AGUGGCAUCUGUGAGCUGC 54 955 AGUGGCAUCUGUGAGCUGC 54 977 GCAGCUCACAGAUGCCACU 303 973 CACUGCCCAGCCCUGGUCA 55 973 CACUGCCCAGCCCUGGUCA 55 995 UGACCAGGGCUGGGCAGUG 304 991 ACCUACAACACAGACACGU 56 991 ACCUACAACACAGACACGU 56 1013 ACGUGUCUGUGUUGUAGGU 305 1009 UUUGAGUCCAUGCCCAAUC 57 1009 UUUGAGUCCAUGCCCAAUC 57 1031 GAUUGGGCAUGGACUCAAA 306 1027 CCCGAGGGCCGGUAUACAU 58 1027 CCCGAGGGCCGGUAUACAU 58 1049 AUGUAUACCGGCCCUCGGG 307 1045 UUCGGCGCCAGCUGUGUGA 59 1045 UUCGGCGCCAGCUGUGUGA 59 1067 UCACACAGCUGGCGCCGAA 308 1063 ACUGCCUGUCCCUACAACU 60 1063 ACUGCCUGUCCCUACAACU 60 1085 AGUUGUAGGGACAGGCAGU 309 1081 UACCUUUCUACGGACGUGG 61 1081 UACCUUUCUACGGACGUGG 61 1103 CCACGUCCGUAGAAAGGUA 310 1099 GGAUCCUGCACCCUCGUCU 62 1099 GGAUCCUGCACCCUCGUCU 62 1121 AGACGAGGGUGCAGGAUCC 311 1117 UGCCCCCUGCACAACCAAG 63 1117 UGCCCCCUGCACAACCAAG 63 1139 CUUGGUUGUGCAGGGGGCA 312 1135 GAGGUGACAGCAGAGGAUG 64 1135 GAGGUGACAGCAGAGGAUG 64 1157 CAUCCUCUGCUGUCACCUC 313 1153 GGAACACAGCGGUGUGAGA 65 1153 GGAACACAGCGGUGUGAGA 65 1175 UCUCACACCGCUGUGUUCC 314 1171 AAGUGCAGCAAGCCCUGUG 66 1171 AAGUGCAGCAAGCCCUGUG 66 1193 CACAGGGCUUGCUGCACUU 315 1189 GCCCGAGUGUGCUAUGGUC 67 1189 GCCCGAGUGUGCUAUGGUC 67 1211 GACCAUAGCACACUCGGGC 316 1207 CUGGGCAUGGAGCACUUGC 68 1207 CUGGGCAUGGAGCACUUGC 68 1229 GCAAGUGCUCCAUGCCCAG 317 1225 CGAGAGGUGAGGGCAGUUA 69 1225 CGAGAGGUGAGGGCAGUUA 69 1247 UAACUGCCCUCACCUCUCG 318 1243 ACCAGUGCCAAUAUCCAGG 70 1243 ACCAGUGCCAAUAUCCAGG 70 1265 CCUGGAUAUUGGCACUGGU 319 1261 GAGUUUGCUGGCUGCAAGA 71 1261 GAGUUUGCUGGCUGCAAGA 71 1283 UCUUGCAGCCAGCAAACUC 320 1279 AAGAUCUUUGGGAGCCUGG 72 1279 AAGAUCUUUGGGAGCCUGG 72 1301 CCAGGCUCCCAAAGAUCUU 321 1297 GCAUUUCUGCCGGAGAGCU 73 1297 GCAUUUCUGCCGGAGAGCU 73 1319 AGCUCUCCGGCAGAAAUGC 322 1315 UUUGAUGGGGACCCAGCCU 74 1315 UUUGAUGGGGACCCAGCCU 74 1337 AGGCUGGGUCCCCAUCAAA 323 1333 UCCAACACUGCCCCGCUCC 75 1333 UCCAACACUGCCCCGCUCC 75 1355 GGAGCGGGGCAGUGUUGGA 324 1351 CAGCCAGAGCAGCUCCAAG 76 1351 CAGCCAGAGCAGCUCCAAG 76 1373 CUUGGAGCUGCUCUGGCUG 325 1369 GUGUUUGAGACUCUGGAAG 77 1369 GUGUUUGAGACUCUGGAAG 77 1391 CUUCCAGAGUCUCAAACAC 326 1387 GAGAUCACAGGUUACCUAU 78 1387 GAGAUCACAGGUUACCUAU 78 1409 AUAGGUAACCUGUGAUCUC 327 1405 UACAUCUCAGCAUGGCCGG 79 1405 UACAUCUCAGCAUGGCCGG 79 1427 CCGGCCAUGCUGAGAUGUA 328 1423 GACAGCCUGGCUGACCUCA 80 1423 GACAGCCUGCCUGACCUCA 80 1445 UGAGGUCAGGCAGGCUGUC 329 1441 AGCGUCUUCCAGAACCUGC 81 1441 AGCGUCUUCCAGAACCUGC 81 1463 GCAGGUUCUGGAAGACGCU 330 1459 CAAGUAAUCCGGGGACGAA 82 1459 CAAGUAAUCCGGGGACGAA 82 1481 UUCGUCCCCGGAUUACUUG 331 1477 AUUCUGCACAAUGGCGCCU 83 1477 AUUCUGCACAAUGGCGCCU 83 1499 AGGCGCCAUUGUGCAGAAU 332 1495 UACUCGCUGACCCUGCAAG 84 1495 UACUCGCUGACCCUGCAAG 84 1517 CUUGCAGGGUCAGCGAGUA 333 1513 GGGCUGGGCAUCAGCUGGC 85 1513 GGGCUGGGCAUCAGCUGGC 85 1535 GCCAGCUGAUGCCCAGCCC 334 1531 CUGGGGCUGCGCUCACUGA 86 1531 CUGGGGCUGCGCUCACUGA 86 1553 UCAGUGAGCGCAGCCCCAG 335 1549 AGGGAACUGGGCAGUGGAC 87 1549 AGGGAACUGGGCAGUGGAC 87 1571 GUCCACUGCCCAGUUCCCU 336 1567 CUGGCCCUCAUCCACCAUA 88 1567 CUGGCCCUCAUCCACCAUA 88 1589 UAUGGUGGAUGAGGGCCAG 337 1585 AACACCCACCUCUGCUUCG 89 1585 AACACCCACCUCUGCUUCG 89 1607 CGAAGCAGAGGUGGGUGUU 338 1603 GUGCACACGGUGCCCUGGG 90 1603 GUGCACACGGUGCCCUGGG 90 1625 CCCAGGGCACCGUGUGCAC 339 1621 GACCAGCUCUUUCGGAACC 91 1621 GACCAGCUCUUUCGGAACC 91 1643 GGUUCCGAAAGAGCUGGUC 340 1639 CCGCACCAAGCUCUGCUCC 92 1639 CCGCACCAAGCUCUGCUCC 92 1661 GGAGCAGAGCUUGGUGCGG 341 1657 CACACUGCCAACCGGCCAG 93 1657 CACACUGCCAACCGGCCAG 93 1679 CUGGCCGGUUGGCAGUGUG 342 1675 GAGGACGAGUGUGUGGGCG 94 1675 GAGGACGAGUGUGUGGGCG 94 1697 CGCCCACACACUCGUCCUC 343 1693 GAGGGCCUGGCCUGCCACC 95 1693 GAGGGCCUGGCCUGCCACC 95 1715 GGUGGCAGGCCAGGCCCUC 344 1711 CAGCUGUGCGCCCGAGGGC 96 1711 CAGCUGUGCGCCCGAGGGC 96 1733 GCCCUCGGGCGCACAGCUG 345 1729 CACUGCUGGGGUCCAGGGC 97 1729 CACUGCUGGGGUCCAGGGC 97 1751 GCCCUGGACCCCAGCAGUG 346 1747 CCCACCCAGUGUGUCAACU 98 1747 CCCACCCAGUGUGUCAACU 98 1769 AGUUGACACACUGGGUGGG 347 1765 UGCAGCCAGUUCCUUCGGG 99 1765 UGCAGCCAGUUCCUUCGGG 99 1787 CCCGAAGGAACUGGCUGCA 348 1783 GGCCAGGAGUGCGUGGAGG 100 1783 GGCCAGGAGUGCGUGGAGG 100 1805 CCUCCACGCACUCCUGGCC 349 1801 GAAUGCCGAGUACUGCAGG 101 1801 GAAUGCCGAGUACUGCAGG 101 1823 CCUGCAGUACUCGGCAUUC 350 1819 GGGCUCCCCAGGGAGUAUG 102 1819 GGGCUCCCCAGGGAGUAUG 102 1841 CAUACUCCCUGGGGAGCCC 351 1837 GUGAAUGCCAGGCACUGUU 103 1837 GUGAAUGCCAGGCACUGUU 103 1859 AACAGUGCCUGGCAUUCAC 352 1855 UUGCCGUGCCACCCUGAGU 104 1855 UUGCCGUGCCACCCUGAGU 104 1877 ACUCAGGGUGGCACGGCAA 353 1873 UGUCAGCCCCAGAAUGGCU 105 1873 UGUCAGCCCCAGAAUGGCU 105 1895 AGCCAUUCUGGGGCUGACA 354 1891 UCAGUGACCUGUUUUGGAC 106 1891 UCAGUGACCUGUUUUGGAC 106 1913 GUCCAAAACAGGUCACUGA 355 1909 CCGGAGGCUGACCAGUGUG 107 1909 CCGGAGGCUGACCAGUGUG 107 1931 CACACUGGUCAGCCUCCGG 356 1927 GUGGCCUGUGCCCACUAUA 108 1927 GUGGCCUGUGCCCACUAUA 108 1949 UAUAGUGGGCACAGGCCAC 357 1945 AAGGACCCUCCCUUCUGCG 109 1945 AAGGACCCUCCCUUCUGCG 109 1967 CGCAGAAGGGAGGGUCCUU 358 1963 GUGGCCCGCUGCCCCAGCG 110 1963 GUGGCCCGCUGCCCCAGCG 110 1985 CGCUGGGGCAGCGGGCCAC 359 1981 GGUGUGAAACCUGACCUCU 111 1981 GGUGUGAAACCUGACCUCU 111 2003 AGAGGUCAGGUUUCACACC 360 1999 UCCUACAUGOCCAUCUGGA 112 1999 UCCUACAUGCCCAUCUGGA 112 2021 UCCAGAUGGGCAUGUAGGA 361 2017 AAGUUUCCAGAUGAGGAGG 113 2017 AAGUUUCCAGAUGAGGAGG 113 2039 CCUCCUCAUCUGGAAACUU 362 2035 GGCGCAUGCCAGCCUUGCC 114 2035 GGCGCAUGCCAGCCUUGCC 114 2057 GGCAAGGCUGGCAUGCGCC 363 2053 CCCAUCAACUGCACCCACU 115 2053 CCCAUCAACUGCACCCACU 115 2075 AGUGGGUGCAGUUGAUGGG 364 2071 UCCUGUGUGGACCUGGAUG 116 2071 UCCUGUGUGGACCUGGAUG 116 2093 CAUCCAGGUCCACACAGGA 365 2089 GACAAGGGCUGCCCCGCCG 117 2089 GACAAGGGCUGCCCCGCCG 117 2111 CGGCGGGGCAGCCCUUGUC 366 2107 GAGCAGAGAGCCAGCCCUC 118 2107 GAGCAGAGAGCCAGCCCUC 118 2129 GAGGGCUGGCUCUCUGCUC 367 2125 CUGACGUCCAUCAUCUCUG 119 2125 CUGACGUCCAUCAUCUCUG 119 2147 CAGAGAUGAUGGACGUCAG 368 2143 GCGGUGGUUGGCAUUCUGC 120 2143 GCGGUGGUUGGCAUUCUGC 120 2165 GCAGAAUGCCAACCACCGC 369 2161 CUGGUCGUGGUCUUGGGGG 121 2161 CUGGUCGUGGUCUUGGGGG 121 2183 CCCCCAAGACCACGACCAG 370 2179 GUGGUCUUUGGGAUCCUCA 122 2179 GUGGUCUUUGGGAUCCUCA 122 2201 UGAGGAUCCCAAAGACCAC 371 2197 AUCAAGCGACGGCAGCAGA 123 2197 AUCAAGCGACGGCAGCAGA 123 2219 UCUGCUGCCGUCGCUUGAU 372 2215 AAGAUCCGGAAGUACACGA 124 2215 AAGAUCCGGAAGUACACGA 124 2237 UCGUGUACUUCCGGAUCUU 373 2233 AUGCGGAGACUGCUGCAGG 125 2233 AUGCGGAGACUGCUGCAGG 125 2255 CCUGCAGCAGUCUCCGCAU 374 2251 GAAACGGAGCUGGUGGAGC 126 2251 GAAACGGAGCUGGUGGAGC 126 2273 GCUCCACCAGCUCCGUUUC 375 2269 CCGCUGACACCUAGCGGAG 127 2269 CCGCUGACACCUAGCGGAG 127 2291 CUCCGCUAGGUGUCAGCGG 376 2287 GCGAUGCCCAACCAGGCGC 128 2287 GCGAUGCCCAACCAGGCGC 128 2309 GCGCCUGGUUGGGCAUCGC 377 2305 CAGAUGCGGAUCCUGAAAG 129 2305 CAGAUGCGGAUCCUGAAAG 129 2327 CUUUCAGGAUCCGCAUCUG 378 2323 GAGACGGAGCUGAGGkAGG 130 2323 GAGACGGAGCUGAGGAAGG 130 2345 CCUUCCUCAGCUCCGUCUC 379 2341 GUGAAGGUGCUUGGAUCUG 131 2341 GUGAAGGUGCUUGGAUCUG 131 2363 CAGAUCCAAGCACCUUCAC 380 2359 GGCGCUUUUGGCACAGUCU 132 2359 GGCGCUUUUGGCACAGUCU 132 2381 AGACUGUGCCAAAAGCGCC 381 2377 UACAAGGGCAUCUGGAUCC 133 2377 UACAAGGGCAUCUGGAUCC 133 2399 GGAUCCAGAUGCCCUUGUA 382 2395 CCUGAUGGGGAGAAUGUGA 134 2395 CCUGAUGGGGAGAAUGUGA 134 2417 UCACAUUCUCCCCAUCAGG 383 2413 AAAAUUCCAGUGGCCAUCA 135 2413 AAAAUUCCAGUGGCCAUCA 135 2435 UGAUGGCCACUGGAAUUUU 384 2431 AAAGUGUUGAGGGAAACA 136 2431 AAAGUGUUGAGGGAAAACA 136 2453 UGUUUUCCCUCAACACUUU 385 2449 ACAUCCCCCAAAGCCAACA 137 2449 ACAUCCCCCAAAGCCAACA 137 2471 UGUUGGCUUUGGGGGAUGU 386 2467 AAAGAAAUCUUAGACGAAG 138 2467 AAAGAAAUCUUAGACGAAG 138 2489 CUUCGUCUAAGAUUUCUUU 387 2485 GCAUACGUGAUGGCUGGUG 139 2485 GCAUACGUGAUGGCUGGUG 139 2507 CACCAGCCAUCACGUAUGC 388 2503 GUGGGCUCCCCAUAUGUCU 140 2503 GUGGGCUCCCCAUAUGUCU 140 2525 AGACAUAUGGGGAGCCCAC 389 2521 UCCCGCCUUCUGGGCAUCU 141 2521 UCCCGCCUUCUGGGCAUCU 141 2543 AGAUGCCCAGAAGGCGGGA 390 2539 UGCCUGACAUCCACGGUGC 142 2539 UGCCUGACAUCCACGGUGC 142 2561 GCACCGUGGAUGUCAGGCA 391 2557 CAGCUGGUGACACAGCUUA 143 2557 CAGCUGGUGACACAGCUUA 143 2579 UAAGCUGUGUCACCAGCUG 392 2575 AUGCCCUAUGGCUGCCUCU 144 2575 AUGCCCUAUGGCUGCCUCU 144 2597 AGAGGCAGCCAUAGGGCAU 393 2593 UUAGACCAUGUCCGGGAAA 145 2593 UUAGACCAUGUCCGGGAAA 145 2615 UUUCCCGGACAUGGUCUAA 394 2611 AACCGCGGACGCCUGGGCU 146 2611 AACCGCGGACGCCUGGGCU 146 2633 AGCCCAGGCGUCCGCGGUU 395 2629 UCCCAGGACCUGCUGAACU 147 2629 UCCCAGGACCUGCUGAACU 147 2651 AGUUCAGCAGGUCCUGGGA 396 2647 UGGUGUAUGCAGAUUGCCA 148 2647 UGGUGUAUGCAGAUUGCCA 148 2669 UGGCAAUCUGCAUACACCA 397 2665 AAGGGGAUGAGCUACCUGG 149 2665 AAGGGGAUGAGCUACCUGG 149 2687 CCAGGUAGCUCAUCCCCUU 398 2683 GAGGAUGUGCGGCUCGUAC 150 2683 GAGGAUGUGCGGCUOGUAC 150 2705 GUACGAGCCGCACAUCCUC 399 2701 CACAGGGACUUGGCCGCUC 151 2701 CACAGGGACUUGGCCGCUC 151 2723 GAGCGGCCAAGUCCCUGUG 400 2719 CGGPACGUGCUGGUCAAGA 152 2719 CGGAACGUGCUGGUCAAGA 152 2741 UCUUGACCAGCACGUUCCG 401 2737 AGUCCCAACCAUGUCAAAA 153 2737 AGUCCCAACCAUGUCAAAA 153 2759 UUUUGACAUGGUUGGGACU 402 2755 AUUACAGACUUCGGGCUGG 154 2755 AUUACAGACUUCGGGCUGG 154 2777 CCAGCCCGAAGUCUGUAAU 403 2773 GCUCGGCUGCUGGACAUUG 155 2773 GCUCGGCUGCUGGACAUUG 155 2795 CAAUGUCCAGCAGCCGAGC 404 2791 GACGAGACAGAGUACCAUG 156 2791 GACGAGACAGAGUACCAUG 156 2813 CAUGGUACUCUGUCUOGUC 405 2809 GCAGAUGGGGGCAAGGUGC 157 2809 GCAGAUGGGGGCAAGGUGC 157 2831 GCACCUUGCCCCCAUCUGC 406 2827 CCCAUCAAGUGGAUGGCGC 158 2827 CCCAUCAAGUGGAUGGCGC 158 2849 GCGCCAUCCACUUGAUGGG 407 2845 CUGGAGUCCAUUCUCCGCC 159 2845 CUGGAGUCCAUUCUCCGCC 159 2867 GGCGGAGAAUGGACUCCAG 408 2863 CGGCGGUUCACCCACCAGA 160 2863 CGGCGGUUCACCCACCAGA 160 2885 UCUGGUGGGUGAACCGCCG 409 2881 AGUGAUGUGUGGAGUUAUG 161 2881 AGUGAUGUGUGGAGUUAUG 161 2903 CAUAACUCCACACAUCACU 410 2899 GGUGUGACUGUGUGGGAGC 162 2899 GGUGUGACUGUGUGGGAGC 162 2921 GCUCCCACACAGUCACACC 411 2917 CUGAUGACUUUUGGGGCCA 163 2917 CUGAUGACUUUUGGGGCCA 163 2939 UGGCCCCAAAAGUCAUCAG 412 2935 AAACCUUACGAUGGGAUCC 164 2935 AAACCUUACGAUGGGAUCC 164 2957 GGAUCCCAUCGUAAGGUUU 413 2953 CCAGCCCGGGAGAUCCCUG 165 2953 CCAGCCCGGGAGAUCCCUG 165 2975 CAGGGAUCUCCCGGGCUGG 414 2971 GACCUGCUGGAAAAGGGGG 166 2971 GACCUGCUGGAAAAGGGGG 166 2993 CCCCCUUUUCCAGCAGGUC 415 2989 GAGCGGCUGCCCCAGCCCC 167 2989 GAGCGGCUGCCCCAGCCCC 167 3011 GGGGCUGGGGCAGCCGCUC 416 3007 CCCAUCUGCACCAUUGAUG 168 3007 CCCAUCUGCACCAUUGAUG 168 3029 CAUCAAUGGUGCAGAUGGG 417 3025 GUCUACAUGAUCAUGGUCA 169 3025 GUCUACAUGAUCAUGGUCA 169 3047 UGACCAUGAUCAUGUAGAC 418 3043 AAAUGUUGGAUGAUUGACU 170 3043 AAAUGUUGGAUGAUUGACU 170 3065 AGUCAAUCAUCCAACAUUU 419 3061 UCUGAAUGUCGGCCAAGAU 171 3061 UCUGAAUGUCGGCCAAGAU 171 3083 AUCUUGGCCGACAUUCAGA 420 3079 UUCCGGGAGUUGGUGUCUG 172 3079 UUCCGGGAGUUGGUGUCUG 172 3101 CAGACACCAACUCCCGGAA 421 3097 GAAUUCUCCCGCAUGGCCA 173 3097 GAAUUCUCCCGCAUGGCCA 173 3119 UGGCCAUGCGGGAGAAUUC 422 3115 AGGGACCCCCAGCGCUUUG 174 3115 AGGGACCCCCAGCGCUUUG 174 3137 CAAAGCGCUGGGGGUCCCU 423 3133 GUGGUCAUCCAGAAUGAGG 175 3133 GUGGUCAUCCAGAAUGAGG 175 3155 CCUCAUUCUGGAUGACCAC 424 3151 GACUUGGGCCCAGCCAGUC 176 3151 GACUUGGGCCCAGCCAGUC 176 3173 GACUGGCUGGGCCCAAGUC 425 3169 CCCUUGGACAGCACCUUCU 177 3169 CCCUUGGACAGCACCUUCU 177 3191 AGAAGGUGCUGUCCAAGGG 426 3187 UACCGCUCACUGCUGGAGG 178 3187 UACCGCUCACUGCUGGAGG 178 3209 CCUCCAGCAGUGAGCGGUA 427 3205 GACGAUGACAUGGGGGACC 179 3205 GACGAUGACAUGGGGGACC 179 3227 GGUCCCCCAUGUCAUCGUC 428 3223 CUGGUGGAUGCUGAGGAGU 180 3223 CUGGUGGAUGCUGAGGAGU 180 3245 ACUCCUCAGCAUCCACCAG 429 3241 UAUCUGGUACCCCAGCAGG 181 3241 UAUCUGGUACCCCAGCAGG 181 3263 CCUGCUGGGGUACCAGAUA 430 3259 GGCUUCUUCUGUCCAGACC 182 3259 GGCUUCUUCUGUCCAGACC 182 3281 GGUCUGGACAGAAGAAGCC 431 3277 CCUGCCCCGGGCGCUGGGG 183 3277 CCUGCCCCGGGCGCUGGGG 183 3299 CCCCAGCGCCCGGGGCAGG 432 3295 GGCAUGGUCCACCACAGGC 184 3295 GGCAUGGUCCACCACAGGC 184 3317 GCCUGUGGUGGACCAUGCC 433 3313 CACCGCAGCUCAUCUACCA 185 3313 CACCGCAGCUCAUCUACCA 185 3335 UGGUAGAUGAGCUGCGGUG 434 3331 AGGAGUGGCGGUGGGGACC 186 3331 AGGAGUGGCGGUGGGGACC 186 3353 GGUCCCCACCGCCACUCCU 435 3349 CUGACACUAGGGCUGGAGC 187 3349 CUGACACUAGGGCUGGAGC 187 3371 GCUCCAGCCCUAGUGUCAG 436 3367 CCCUCUGAAGAGGAGGCCC 188 3367 CCCUCUGAAGAGGAGGCCC 188 3389 GGGCCUCCUCUUCAGAGGG 437 3385 CCCAGGUCUCCACUGGCAC 189 3385 CCCAGGUCUCCACUGGCAC 189 3407 GUGCCAGUGGAGACCUGGG 438 3403 CCCUCCGAAGGGGCUGGCU 190 3403 CCCUCCGAAGGGGCUGGCU 190 3425 AGCCAGCCCCUUCGGAGGG 439 3421 UCCGAUGUAUUUGAUGGUG 191 3421 UCCGAUGUAUUUGAUGGUG 191 3443 CACCAUCPAAUACAUCGGA 440 3439 GACCUGGGAAUGGGGGCAG 192 3439 GACCUGGGAAUGGGGGCAG 192 3461 CUGCCCCCAUUCCCAGGUC 441 3457 GCCAAGGGGCUGCAAAGCC 193 3457 GCCAAGGGGCUGCAAAGCC 193 3479 GGCUUUGCAGCCCCUUGGC 442 3475 CUCCCCACACAUGACCCCA 194 3475 CUCCCCACACAUGACCCCA 194 3497 UGGGGUCAUGUGUGGGGAG 443 3493 AGCCCUCUACAGCGGUACA 195 3493 AGCCCUCUACAGCGGUACA 195 3515 UGUACCGCUGUAGAGGGCU 444 3511 AGUGAGGACCOCACAGUAC 196 3511 AGUGAGGACCCCACAGUAC 196 3533 GUACUGUGGGGUCCUCACU 445 3529 CCCCUGCCCUCUGAGACUG 197 3529 CCCCUGCCCUCUGAGACUG 197 3551 CAGUCUCAGAGGGCAGGGG 446 3547 GAUGGCUACGUUGCCCCCC 198 3547 GAUGGCUACGUUGCCCCCC 198 3569 GGGGGGCAACGUAGCCAUC 447 3565 CUGACCUGCAGCCCCCAGC 199 3565 CUGACCUGCAGCCCCCAGC 199 3587 GCUGGGGGCUGCAGGUCAG 448 3583 CCUGAAUAUGUGAACCAGC 200 3583 CCUGAAUAUGUGAACCAGC 200 3605 GCUGGUUCACAUAUUCAGG 449 3601 CCAGAUGUUCGGCCCCAGC 201 3601 CCAGAUGUUCGGCCCCAGC 201 3623 GCUGGGGCCGAACAUCUGG 450 3619 CCCCCUUCGCCCCGAGAGG 202 3619 CCCCCUUCGCCCCGAGAGG 202 3641 CCUCUCGGGGCGAAGGGGG 451 3637 GGCCCUCUGCCUGCUGCCC 203 3637 GGCCCUCUGCCUGCUGCCC 203 3659 GGGCAGCAGGCAGAGGGCC 452 3655 CGACCUGCUGGUGCCACUC 204 3655 CGACCUGCUGGUGCCACUC 204 3677 GAGUGGCACCAGCAGGUCG 453 3673 CUGGAAAGGCCCAAGACUC 205 3673 CUGGAAAGGCCCAAGACUC 205 3695 GAGUCUUGGGCCUUUCCAG 454 3691 CUCUCCCCAGGGAAGAAUG 206 3691 CUCUCCCCAGGGAAGAAUG 206 3713 CAUUCUUCCCUGGGGAGAG 455 3709 GGGGUCGUCAAAGACGUUU 207 3709 GGGGUCGUCAAAGACGUUU 207 3731 AAACGUCUUUGACGACCCC 456 3727 UUUGCCUUUGGGGGUGCCG 208 3727 UUUGCCUUUGGGGGUGCCG 208 3749 CGGCACCCCCkAAGGCAAA 457 3745 GUGGAGAACCCCGAGUACU 209 3745 GUGGAGAACCCCGAGUACU 209 3767 AGUACUCGGGGUUCUCCAC 458 3763 UUGACACCCCAGGGAGGAG 210 3763 UUGACACCCCAGGGAGGAG 210 3785 CUCCUCCCUGGGGUGUCAA 459 3781 GCUGCCCCUCAGCCCCACC 211 3781 GCUGCCCCUCAGCCCCACC 211 3803 GGUGGGGCUGAGGGGCAGC 460 3799 CCUCCUCCUGCCUUCAGCC 212 3799 CCUCCUCCUGCCUUCAGCC 212 3821 GGCUGAAGGCAGGAGGAGG 461 3817 CCAGCCUUCGACAACCUCU 213 3817 CCAGCCUUCGACAACCUCU 213 3839 AGAGGUUGUCGAAGGCUGG 462 3835 UAUUACUGGGACCAGGACC 214 3835 UAUUACUGGGACCAGGACC 214 3857 GGUCCUGGUCCCAGUAAUA 463 3853 CCACCAGAGCGGGGGGCUC 215 3853 CCACCAGAGCGGGGGGCUC 215 3875 GAGCCCCCCGCUCUGGUGG 464 3871 CCACCCAGCACCUUCAAAG 216 3871 CCACCCAGCACCUUCAAAG 216 3893 CUUUGAAGGUGCUGGGUGG 465 3889 GGGACACCUACGGCAGAGA 217 3889 GGGACACCUACGGCAGAGA 217 3911 UCUCUGCCGUAGGUGUCCC 466 3907 AACCCAGAGUACCUGGGUC 218 3907 AACCCAGAGUACCUGGGUC 218 3929 GACCCAGGUACUCUGGGUU 467 3925 CUGGACGUGCCAGUGUGAA 219 3925 CUGGACGUGCCAGUGUGAA 219 3947 UUCACACUGGCACGUCCAG 468 3943 ACCAGAAGGCCAAGUCCGC 220 3943 ACCAGAAGGCCAAGUCCGC 220 3965 GCGGACUUGGCCUUCUGGU 469 3961 CAGAAGCCCUGAUGUGUCC 221 3961 CAGAAGCCCUGAUGUGUCC 221 3983 GGACACAUCAGGGCUUCUG 470 3979 CUCAGGGAGCAGGGAAGGC 222 3979 CUCAGGGAGCAGGGAAGGC 222 4001 GCCUUCCCUGCUCCCUGAG 471 3997 CCUGACUUCUGCUGGCAUC 223 3997 CCUGACUUCUGCUGGCAUC 223 4019 GAUGCCAGCAGAAGUCAGG 472 4015 CAAGAGGUGGGAGGGCCCU 224 4015 CAAGAGGUGGGAGGGCCCU 224 4037 AGGGCCCUCCCACCUCUUG 473 4033 UCCGACCACUUCCAGGGGA 225 4033 UCCGACCACUUCCAGGGGA 225 4055 UCCCCUGGAAGUGGUCGGA 474 4051 AACCUGCCAUGCCAGGAAC 226 4051 AACCUGCCAUGCCAGGAAC 226 4073 GUUCCUGGCAUGGCAGGUU 475 4069 CCUGUCCUAAGGAACCUUC 227 4069 CCUGUCCUAAGGAACCUUC 227 4091 GAAGGUUCCUUAGGACAGG 476 4087 CCUUCCUGCUUGAGUUCCC 228 4087 CCUUCCUGCUUGAGUUCCC 228 4109 GGGAACUCAAGCAGGAAGG 477 4105 CAGAUGGCUGGAAGGGGUC 229 4105 CAGAUGGCUGGAAGGGGUC 229 4127 GACCCCUUCCAGCCAUCUG 478 4123 CCAGCCUCGUUGGAAGAGG 230 4123 CCAGCCUCGUUGGAAGAGG 230 4145 CCUCUUCCAACGAGGCUGG 479 4141 GAACAGCACUGGGGAGUCU 231 4141 GAACAGCACUGGGGAGUCU 231 4163 AGACUCCCCAGUGCUGUUC 480 4159 UUUGUGGAUUCUGAGGCCC 232 4159 UUUGUGGAUUCUGAGGCCC 232 4181 GGGCCUCAGAAUCCACkAA 481 4177 CUGCCCAAUGAGACUCUAG 233 4177 CUGCCCAAUGAGACUCUAG 233 4199 CUAGAGUCUCAUUGGGCAG 482 4195 GGGUCCAGUGGAUGCCACA 234 4195 GGGUCCAGUGGAUGCCACA 234 4217 UGUGGCAUCCACUGGACCC 483 4213 AGCCCAGCUUGGCCCUUUC 235 4213 AGCCCAGCUUGGCCCUUUC 235 4235 GAAAGGGCCAAGCUGGGCU 484 4231 CCUUCCAGAUCCUGGGUAC 236 4231 CCUUCCAGAUCCUGGGUAC 236 4253 GUACCCAGGAUCUGGAAGG 485 4249 CUGAAAGCCUUAGGGAAGC 237 4249 CUGAAAGCCUUAGGGAAGC 237 4271 GCUUCCCUAAGGCUUUCAG 486 4267 CUGGCCUGAGAGGGGAAGC 238 4267 CUGGCCUGAGAGGGGAAGC 238 4289 GCUUCCCCUCUCAGGCCAG 487 4285 CGGCCCUAAGGGAGUGUCU 239 4285 CGGCCCUAAGGGAGUGUCU 239 4307 AGACACUCCCUUAGGGCCG 488 4303 UAAGAACAAAAGCGACCCA 240 4303 UAAGAACAAAAGCGACCCA 240 4325 UGGGUCGCUUUUGUUCUUA 489 4321 AUUCAGAGACUGUCCCUGA 241 4321 AUUCAGAGACUGUCCCUGA 241 4343 UCAGGGACAGUCUCUGAAU 490 4339 AAACCUAGUACUGCCCCCC 242 4339 AAACCUAGUACUGCCCCCC 242 4361 GGGGGGCAGUACUAGGUUU 491 4357 CAUGAGGAAGGAACAGCAA 243 4357 CAUGAGGAAGGAACAGCAA 243 4379 UUGCUGUUCCUUCCUCAUG 492 4375 AUGGUGUCAGUAUCCAGGC 244 4375 AUGGUGUCAGUAUCCAGGC 244 4397 GCCUGGAUACUGACACCAU 493 4393 CUUUGUACAGAGUGCUUUU 245 4393 CUUUGUACAGAGUGCUUUU 245 4415 AAAAGCACUCUGUACAAAG 494 4411 UCUGUUUAGUUUUUACUUU 246 4411 UCUGUUUAGUUUUUACUUU 246 4433 AAAGUAAAAACUAAACAGA 495 4429 UUUUUGUUUUGUUUUUUUA 247 4429 UUUUUGUUUUGUUUUUUUA 247 4451 UAAAAAACAAAACAAAAAA 496 4447 AAGAUGAAAUAAAGACCCA 248 4447 AAAGAUGAAAUAAAGACCC 248 4469 GGGUCUUUAUUUCAUCUUU 497 4455 AAUAAAGACCCAGGGGGAG 249 4455 AAUAAAGACCCAGGGGGAG 249 4477 CUCCCCCUGGGUCUUUAUU 498 HSERB2R (X03363)

[0312] TABLE II +HZ,1 HER2 Synthetic siNA constructs SEQ RPI# Aliases Sequence ID# 25245 RPI 17763 Her2Neu AS as siNA Str 2 BUCCAUGGUGOUCACUGOGGOUB 499 (antisense) 25246 RPI 17763 Her2Neu AS as siNA Str 1 BAGCCGCAGUGAGCACCAUGGAB 500 (sense) 25247 RPI 17763 Her2Neu AS as siNA Str 1 BAGGUACCACGAGUGACGCCGAB 501 (sense) Inverted control 25248 RPI 17763 Her2Neu AS as siNA Str 1 BUCGGCGUCACUCGUGGUACCUB 502 (sense) Inverted control compliment 25822 RPI 17763 Her2Neu AS as siNA Str 2 UCCAUGGUGCUCACUGCGGCUUU 503 (antisense)+2U overhang 25823 RPI 17763 Her2Neu AS as siNA Str 1 AGCCGCAGUGAGCACCAUGGAUU 504 (sense)+2U overhang 25842 RPI 17763 Her2Neu AS as siNA Str 2 BUCCAUGGUGCUCACUGCGGCUUUB 505 (antisense)+2U overhang 25843 RPI 17763 Her2Neu AS as siNA Str 1 BAGCCGCAGUGAGCACCAUGGAUUB 506 (sense)+2U overhang 28262 Her2.1.sense Str1 UGGGGUCGUCAAAGACGUUTT 507 28263 Her2.1.antisense Str2 AACGUCUUUGACGACCCCATT 508 28264 Her2.1.sense Str1 inverted UUGCAGAAACUGCUGGGGUTT 509 28265 Her2.1.antisense Str2 inverted ACCCCAGCAGUUUCUGCAATT 510 28266 Her2.2.sense Str1 GGUGCUUGGAUCUGGCGCUTT 511 28267 Her2.2.antisense Str2 AGCGCCAGAUCCAAGCACCTT 512 28268 Her2.2.sense Str1 inverted UCGCGGUCUAGGUUCGUGGTT 513 28269 Her2.2.antisense Str2 inverted CCACGAACCUAGACCGCGATT 514 28270 Her2.3.sense Str1 GAUCUUUGGGAGCCUGGCATT 515 28271 Her2.3.antisense Str2 UGCCAGGCUCCCAAAGAUCTT 516 28272 Her2.3.sense Str1 inverted ACGGUCCGAGGGUUUCUAGTT 517 28273 Her2.3.antisense Str2 inverted CUAGAAACCCUCGGACCGUTT 518 29989 Her2.2.sense Str1 (site 2344) G_(S)G_(S)u_(S)G_(S)cuuGGAucuGGcG_(S)csu_(S)T_(S)T 519 29990 Her2.2.antisense Str2 A_(S)G_(S)C_(S)G_(S)C_(S)CAGAUCCAAGCACCT_(S)T 520 29991 Her2.2.sense Str1 (site 2344) G_(S)G_(S)U_(S)G_(S)C_(S)UUGGAUCUGGCGCUT_(S)T 521 29992 Her2.2.sense Str1 (site 2344) G_(S)G_(S)u_(S)G_(S)cuuGGAucuGGcGcuTTB 522 29993 Her2.2.antisense Str2 A_(S)G_(S)C_(S)G_(S)C_(S)C_(S)A_(S)G_(S)A_(S)U_(S)C_(S)C_(S)A_(S)A_(S)G_(S)C_(S)A_(S)C_(S)C_(S)T_(S)T 523 29994 Her2.2.antisense Str2 A_(S)G_(S)C_(S)G_(S)C_(S)C_(S)A_(S)G_(S)A_(S)U_(S)CCAAGCACCT_(S)T 524 29995 Her2.2.antisense Str2 A_(S)G_(S)C_(S)G_(S)C_(S)C_(S)A_(S)G_(S)A_(S)U_(S)C_(S)C_(S)A_(S)A_(S)GCACCT_(S)T 525 29996 Her2.2.sense Str1 inverted u_(S)c_(S)G_(S)c_(S)GGucuAGGuucGu_(S)G_(S)G_(S)T_(S)AT 526 29997 Her2.2.sense Str1 inverted U_(S)C_(S)G_(S)C_(S)G_(S)GUCUAGGUUCGUGGT_(S)T 527 29998 Her2.2.sense Str1 inverted u_(S)c_(S)G_(S)c_(S)GGucuAGGuucGuGGTTB 528 29999 Her2.2.antisense Str2 inverted C_(S)C_(S)A_(S)C_(S)G_(S)AACCUAGACCGCGAT_(S)T 529 30000 Her2.2.antisense Str2 inverted C_(S)C_(S)A_(S)C_(S)G_(S)A_(S)A_(S)C_(S)C_(S)U_(S)A_(S)G_(S)A_(S)C_(S)C_(S)G_(S)C_(S)G_(S)A_(S)T_(S)T 530 30001 Her2.2.antisense Str2 inverted C_(S)C_(S)A_(S)C_(S)G_(S)A_(S)A_(S)C_(S)C_(S)U_(S)AGACCGCGAT_(S)T 531 30002 Her2.2.antisense Str2 inverted C_(S)C_(S)A_(S)C_(S)G_(S)A_(S)A_(S)C_(S)C_(S)U_(S)A_(S)G_(S)A_(S)C_(S)CGCGAT_(S)T 532

[0313] TABLE III HER2 Synthetic Sequences with all RNA counterparts and Target Sequences Target Seq Pos Target ID Aliases Sequence Seq ID 986 CGUGUCUGUGUUGUAGGUGACCA 533 HER2:988U21 siNA GUCACCUACAACA0AGACACG 537 1882 AAACAGGUCACUGAGCCAUUCUG 534 HER2:1884U21 siN AGAAUGGCUOAGUGACOUGUUU 538 3128 CUCAUUCUGGAUGACCACAAAGC 535 HER2:3130U21 siNA UUUGUGGUCAUCCAGAAUGAG 539 3877 GUAGGUGUCCCUUUGAAGGUGCU 536 HER2:3879U21 siNA CACCUUCAAAGGGACACCUAC 540 986 CGUGUCUGUGUUGUAGGUGACCA 533 HER2:1006L21 siNA (988C) UGUCUGUGUUGUAGGUGACCA 541 1882 AAACAGGUCACUGAGCCAUUCUG 534 HER2:1902L21 siNA (1884C) ACAGGUCACUGAGCCAUUCUG 542 3128 CUCAUUCUGGAUGACCACAAAGC 535 HER2:3148L21 siNA (3130C) CAUUCUGGAUGACCACXAAGC 543 3877 GUAGGUGUCCCUUUGAAGGUGCU 536 HER2:3897L21 siNA (3879C) AGGUGUCCCUUUGAAGGUGCU 544 986 CGUGUCUGUGUUGUAGGUGACCA 533 HER2:988U21 siNA stab4 B GucAccuAcAAcAcAGAcAcG B 545 1882 AAACAGGUCACUGAGCCAUUCUG 534 HER2:1884U21 siNA stab4 B GAAuGGcucAGuGAccuGuuu B 546 3128 CUCAUUCUGGAUGACCACAAAGC 535 HER2:3130U21 siNA stab4 B uuuGuGGucAuccAGAAuGAG B 547 3877 GUAGGUGUCCCUUUGAAGGUGCU 536 HER2:3879U21 siNA stab4 B cAccuucAAAGGGAcAccuAc B 548 986 CGUGUCUGUGUUGUAGGUGACCA 533 HER2:1006L21 siNA (988C) stab5 uGucuGuGuuGuAGGuGAcTsT 549 1882 AAACAGGUCACUGAGCCAUUCUG 534 HER2:1902L21 siNA (1884C) stab5 AcAGGucAcuGAGccAuucTsT 550 3128 CUCAUUCUGGAUGA0CACAAAGC 535 HER2:3148L21 siNA (3130C) stab5 cAuucuGGAuGAccAcAAATsT 551 3877 GUAGGUGUCCCUUUGAAGGUGCU 536 HER2:3897L21 siNA (3879C) stab5 AGGuGucccuuuGAAGGuGTsT 552tz,1/64

[0314] TABLE IV EGFR (HER1) siNA and Target Sequences Seq Seq Seq Pos Target Sequence ID UPos Upper seq ID LPos Lower seq ID 3 CGCGCUGCGCCGGAGUCCC 553 3 CGCGCUGCGCCGGAGUCCC 553 21 GGGACUCCGGCGCAGCGCG 860 21 CGAGCUAGCCCCGGCGCCG 554 21 CGAGCUAGCCCCGGCGCCG 554 39 CGGCGCCGGGGCUAGCUCG 861 39 GCCGCCGCCCAGACCGGAC 555 39 GCCGCCGCCCAGACCGGAC 555 57 GUCCGGUCUGGGCGGCGGC 862 57 CGACAGGCCACCUCGUCGG 556 57 CGACAGGCCACCUCGUCGG 556 75 CCGACGAGGUGGCCUGUCG 863 75 GCGUCCGCCCGAGUCCCCG 557 75 GCGUCCGCCCGAGUCCCCG 557 93 CGGGGACUCGGGCGGACGC 864 93 GCCUCGCCGCCAACGCCAC 558 93 GCCUCGCCGCCAACGCCAC 558 111 GUGGCGUUGGCGGCGAGGC 865 111 CAACCACCGCGCACGGCCC 559 111 CAACCACCGCGCACGGCCC 559 129 GGGCCGUGCGCGGUGGUUG 866 129 CCCUGACUCCGUCCAGUAU 560 129 CCCUGACUCCGUCCAGUAU 560 147 AUACUGGACGGAGUCAGGG 867 147 UUGAUCGGGAGAGCCGGAG 561 147 UUGAUCGGGAGAGCCGGAG 561 165 CUCCGGCUCUCCCGAUCAA 868 165 GCGAGCUCUUCGGGGAGCA 562 165 GCGAGCUCUUCGGGGAGCA 562 183 UGCUCCCCGAAGAGCUCGC 869 183 AGCGAUGCGACCCUCCGGG 563 183 AGCGAUGCGACCCUCCGGG 563 201 CCCGGAGGGUCGCAUCGCU 870 201 GACGGCCGGGGCAGCGCUC 564 201 GACGGCCGGGGCAGCGCUC 564 219 GAGCGCUGCCCCGGCCGUC 871 219 CCUGGCGCUGCUGGCUGCG 565 219 CCUGGCGCUGCUGGCUGCG 565 237 CGCAGCCAGCAGCGCCAGG 872 237 GCUCUGCCCGGCGAGUCGG 566 237 GCUCUGCCCGGCGAGUCGG 566 255 CCGACUCGCCGGGCAGAGC 873 255 GGCUCUGGAGGAAAAGAAA 567 255 GGCUCUGGAGGAAAAGAAA 567 273 UUUCUUUUCCUCCAGAGCC 874 273 AGUUUGCCAAGGCACGAGU 568 273 AGUUUGCCAAGGCACGAGU 568 291 ACUCGUGCCUUGGCAAACU 875 291 UAACAAGCUCACGCAGUUG 569 291 UAACAAGCUCACGCAGUUG 569 309 CAACUGCGUGAGCUUGUUA 876 309 GGGCACUUUUGAAGAUCAU 570 309 GGGCACUUUUGAAGAUCAU 570 327 AUGAUCUUCAAAAGUGCCC 877 327 UUUUCUCAGCCUCCAGAGG 571 327 UUUUCUCAGCCUCCAGAGG 571 345 CCUCUGGAGGCUGAGAAAA 878 345 GAUGUUCAAUAACUGUGAG 572 345 GAUGUUCAAUAACUGUGAG 572 363 CUCACAGUUAUUGAACAUC 879 363 GGUGGUCCUUGGGAAUUUG 573 363 GGUGGUCCUUGGGAAUUUG 573 381 CAAAUUCCCAAGGACCACC 880 381 GGAAAUUACCUAUGUGCAG 574 381 GGAAAUUACCUAUGUGCAG 574 399 CUGCACAUAGGUAAUUUCC 881 399 GAGGAAUUAUGAUCUUUCC 575 399 GAGGAAUUAUGAUCUUUCC 575 417 GGAAAGAUCAUAAUUCCUC 882 417 CUUCUUAAAGACCAUCCAG 576 417 CUUCUUAAAGACCAUCCAG 576 435 CUGGAUGGUCUUUAAGAAG 883 435 GGAGGUGGCUGGUUAUGUC 577 435 GGAGGUGGCUGGUUAUGUC 577 453 GACAUAACCAGCCACCUCC 884 453 CCUCAUUGCCCUCAACACA 578 453 CCUCAUUGCCCUCAACACA 578 471 UGUGUUGAGGGCAAUGAGG 885 471 AGUGGAGCGAAUUCCUUUG 579 471 AGUGGAGCGAAUUCCUUUG 579 489 CAAAGGAAUUCGCUCCACU 886 489 GGAAAACCUGCAGAUCAUC 580 489 GGAAAACCUGCAGAUCAUC 580 507 GAUGAUCUGCAGGUUUUCC 887 507 CAGAGGAAAUAUGUACUAC 581 507 CAGAGGAAAUAUGUACUAC 581 525 GUAGUACAUAUUUCCUCUG 888 525 CGAAAAUUCCUAUGCCUUA 582 525 CGAAAAUUCCUAUGCCUUA 582 543 UAAGGCAUAGGAAUUUUCG 889 543 AGCAGUCUUAUCUAACUAU 583 543 AGCAGUCUUAUCUAACUAU 583 561 AUAGUUAGAUAAGACUGCU 890 561 UGAUGCAAAUAAAACCGGA 584 561 UGAUGCAAAUAAAACCGGA 584 579 UCCGGUUUUAUUUGCAUCA 891 579 ACUGAAGGAGCUGCCCAUG 585 579 ACUGAAGGAGCUGCCCAUG 585 597 CAUGGGCAGCUCCUUCAGU 892 597 GAGAAAUUUACAGGAAAUC 586 597 GAGAAAUUUACAGGAAAUC 586 615 GAUUUCCUGUAAAUUUCUC 893 615 CCUGCAUGGCGCCGUGCGG 587 615 CCUGCAUGGCGCCGUGCGG 587 633 CCGCACGGCGCCAUGCAGG 894 633 GUUCAGCAACAACCCUGCC 588 633 GUUCAGCAACAACCCUGCC 588 651 GGCAGGGUUGUUGCUGAAC 895 651 CCUGUGCAACGUGGAGAGC 589 651 CCUGUGCAACGUGGAGAGC 589 669 GCUCUCCACGUUGCACAGG 896 669 CAUCCAGUGGCGGGACAUA 590 669 CAUCCAGUGGCGGGACAUA 590 687 UAUGUCCCGCCACUGGAUG 897 687 AGUCAGCAGUGACUUUCUC 591 687 AGUCAGCAGUGACUUUCUC 591 705 GAGAAAGUCACUGCUGACU 898 705 CAGCAACAUGUCGAUGGAC 592 705 CAGCAACAUGUCGAUGGAC 592 723 GUCCAUCGACAUGUUGCUG 899 723 CUUCCAGAACCACCUGGGC 593 723 CUUCCAGAACCACCUGGGC 593 741 GCCCAGGUGGUUCUGGAAG 900 741 CAGCUGCCAAAAGUGUGAU 594 741 CAGCUGCCAAAAGUGUGAU 594 759 AUCACACUUUUGGCAGCUG 901 759 UCCAAGCUGUCCCAAUGGG 595 759 UCCAAGCUGUCCCAAUGGG 595 777 CCCAUUGGGACAGCUUGGA 902 777 GAGCUGCUGGGGUGCAGGA 596 777 GAGCUGCUGGGGUGCAGGA 596 795 UCCUGCACCCCAGCAGCUC 903 795 AGAGGAGAACUGCCAGAAA 597 795 AGAGGAGAACUGCCAGAAA 597 813 UUUCUGGCAGUUCUCCUCU 904 813 ACUGACCAAAAUCAUCUGU 598 813 ACUGACCAAAAUCAUCUGU 598 831 ACAGAUGAUUUUGGUCAGU 905 831 UGCCCAGCAGUGCUCCGGG 599 831 UGCCCAGCAGUGCUCCGGG 599 849 CCCGGAGCACUGCUGGGCA 906 849 GCGCUGCCGUGGCAAGUCC 600 849 GCGCUGCCGUGGCAAGUCC 600 867 GGACUUGCCACGGCAGCGC 907 867 CCCCAGUGACUGOUGCCAC 601 867 CCCCAGUGACUGCUGCCAC 601 885 GUGGCAGCAGUCACUGGGG 908 885 CAACCAGUGUGCUGCAGGC 602 885 CAACCAGUGUGCUGCAGGC 602 903 GCCUGCAGCACACUGGUUG 909 903 CUGCACAGGCCCCCGGGAG 603 903 CUGCACAGGCCCCCGGGAG 603 921 CUCCCGGGGGCCUGUGCAG 910 921 GAGCGACUGCCUGGUCUGC 604 921 GAGCGACUGCCUGGUCUGC 604 939 GCAGACCAGGCAGUCGCUC 911 939 CCGCAAAUUCCGAGACGAA 605 939 CCGCAAAUUCCGAGACGAA 605 957 UUCGUCUCGGAAUUUGCGG 912 957 AGCCACGUGCAAGGACACC 606 957 AGCCACGUGCAAGGACACC 606 975 GGUGUCCUUGCACGUGGCU 913 975 CUGCCCCCCACUCAUGCUC 607 975 CUGCCCCCCACUCAUGCUC 607 993 GAGCAUGAGUGGGGGGCAG 914 993 CUACAACCCCACCACGUAC 608 993 CUACAACCCCACCACGUAC 608 1011 GUACGUGGUGGGGUUGUAG 915 1011 CCAGAUGGAUGUGAACCCC 609 1011 CCAGAUGGAUGUGAACCCC 609 1029 GGGGUUCACAUCCAUCUGG 916 1029 CGAGGGCAAAUACAGCUUU 610 1029 CGAGGGCAAAUACAGCUUU 610 1047 AAAGCUGUAUUUGCCCUCG 917 1047 UGGUGCCACCUGCGUGAAG 611 1047 UGGUGCCACCUGCGUGAAG 611 1065 CUUCACGCAGGUGGCACCA 918 1065 GAAGUGUCCCCGUAAUUAU 612 1065 GAAGUGUCCCCGUAAUUAU 612 1083 AUAAUUACGGGGACACUUC 919 1083 UGUGGUGACAGAUCACGGC 613 1083 UGUGGUGACAGAUCACGGC 613 1101 GCCGUGAUCUGUCACCACA 920 1101 CUCGUGCGUCCGAGCCUGU 614 1101 CUCGUGCGUCCGAGCCUGU 614 1119 ACAGGCUCGGACGCACGAG 921 1119 UGGGGCCGACAGCUAUGAG 615 1119 UGGGGCCGACAGCUAUGAG 615 1137 CUCAUAGCUGUCGGCCCCA 922 1137 GAUGGAGGAAGACGGCGUC 616 1137 GAUGGAGGAAGACGGCGUC 616 1155 GACGCCGUCUUCCUCCAUC 923 1155 CCGCAAGUGUAAGAAGUGC 617 1155 CCGCAAGUGUAAGAAGUGC 617 1173 GCACUUCUUACACUUGCGG 924 1173 CGAAGGGCCUUGCCGCAAA 618 1173 CGAAGGGCCUUGCCGCAAA 618 1191 UUUGCGGCAAGGCCCUUCG 925 1191 AGUGUGUAACGGAAUAGGU 619 1191 AGUGUGUAACGGAAUAGGU 619 1209 ACCUAUUCCGUUACACACU 926 1209 UAUUGGUGAAUUUAAAGAC 620 1209 UAUUGGUGAAUUUAAAGAC 620 1227 GUCUUUAAAUUCACCAAUA 927 1227 CUCACUCUCCAUAAAUGCU 621 1227 CUCACUCUCCAUAAAUGCU 621 1245 AGCAUUUAUGGAGAGUGAG 928 1245 UACGAAUAUUAAACACUUC 622 1245 UACGAAUAUUAAACACUUC 622 1263 GAAGUGUUUAAUAUUCGUA 929 1263 CAAAAACUGCACCUCCAUC 623 1263 CAAAAACUGCACCUCCAUC 623 1281 GAUGGAGGUGCAGUUUUUG 930 1281 CAGUGGCGAUCUCCACAUC 624 1281 CAGUGGCGAUCUCCACAUC 624 1299 GAUGUGGAGAUCGCCACUG 931 1299 CCUGCCGGUGGCAUUUAGG 625 1299 CCUGCCGGUGGCAUUUAGG 625 1317 CCUAAAUGCCACCGGCAGG 932 1317 GGGUGACUCCUUCACACAU 626 1317 GGGUGACUCCUUCACACAU 626 1335 AUGUGUGAAGGAGUCACCC 933 1335 UACUCCUCCUCUGGAUCCA 627 1335 UACUCCUCCUCUGGAUCCA 627 1353 UGGAUCCAGAGGAGGAGUA 934 1353 ACAGGAACUGGAUAUUCUG 628 1353 ACAGGAACUGGAUAUUCUG 628 1371 CAGAAUAUCCAGUUCCUGU 935 1371 GAAAACCGUAAAGGAAAUC 629 1371 GAAAACCGUAAAGGAAAUC 629 1389 GAUUUCCUUUACGGUUUUC 936 1389 CACAGGGUUUUUGCUGAUU 630 1389 CACAGGGUUUUUGCUGAUU 630 1407 AAUCAGCAAAAACCCUGUG 937 1407 UCAGGCUUGGCCUGAAAAC 631 1407 UCAGGCUUGGCCUGAAAAC 631 1425 GUUUUCAGGCCAAGCCUGA 938 1425 CAGGACGGACCUCCAUGCC 632 1425 CAGGACGGACCUCCAUGCC 632 1443 GGCAUGGAGGUCCGUCCUG 939 1443 CUUUGAGAACCUAGAAAUC 633 1443 CUUUGAGAACCUAGAAAUC 633 1461 GAUUUCUAGGUUCUCAAAG 940 1461 CAUACGCGGCAGGACCAAG 634 1461 CAUACGCGGCAGGACCAAG 634 1479 CUUGGUCCUGCCGCGUAUG 941 1479 GCAACAUGGUCAGUUUUCU 635 1479 GCAACAUGGUCAGUUUUCU 635 1497 AGAAAACUGACCAUGUUGC 942 1497 UCUUGCAGUCGUCAGCCUG 636 1497 UCUUGCAGUCGUCAGCCUG 636 1515 CAGGCUGACGACUGCAAGA 943 1515 GAACAUAACAUCCUUGGGA 637 1515 GAACAUAACAUCCUUGGGA 637 1533 UCCCAAGGAUGUUAUGUUC 944 1533 AUUACGCUCCCUCAAGGAG 638 1533 AUUACGCUCCCUCAAGGAG 638 1551 CUCCUUGAGGGAGCGUAAU 945 1551 GAUAAGUGAUGGAGAUGUG 639 1551 GAUAAGUGAUGGAGAUGUG 639 1569 CACAUCUCCAUCACUUAUC 946 1569 GAUAAUUUCAGGAAACAAA 640 1569 GAUAAUUUCAGGAAACAAA 640 1587 UUUGUUUCCUGAAAUUAUC 947 1587 AAAUUUGUGCUAUGCAAAU 641 1587 AAAUUUGUGCUAUGCAAAU 641 1605 AUUUGCAUAGCACAAAUUU 948 1605 UACAAUAAACUGGAAAAAA 642 1605 UACAAUAAACUGGAAAAAA 642 1623 UUUUUUCCAGUUUAUUGUA 949 1623 ACUGUUUGGGACCUCCGGU 643 1623 ACUGUUUGGGACCUCCGGU 643 1641 ACCGGAGGUCCCAAACAGU 950 1641 UCAGAAAACCAAAAUUAUA 644 1641 UCAGAAAACCAAAAUUAUA 644 1659 UAUAAUUUUGGUUUUCUGA 951 1659 AAGCAACAGAGGUGAAAAC 645 1659 AAGCAACAGAGGUGAAAAC 645 1677 GUUUUCACCUCUGUUGCUU 952 1677 CAGCUGCAAGGCCACAGGC 646 1677 CAGCUGCAAGGCCACAGGC 646 1695 GCCUGUGGCCUUGCAGCUG 953 1695 CCAGGUCUGCCAUGCCUUG 647 1695 CCAGGUCUGCCAUGCCUUG 647 1713 CAAGGCAUGGCAGACCUGG 954 1713 GUGCUCCCCCGAGGGCUGC 648 1713 GUGCUCCCCCGAGGGCUGC 648 1731 GCAGCCCUCGGGGGAGCAC 955 1731 CUGGGGCCCGGAGCCCAGG 649 1731 CUGGGGCCCGGAGCCCAGG 649 1749 CCUGGGCUCCGGGCCCCAG 956 1749 GGACUGCGUCUCUUGCCGG 650 1749 GGACUGCGUCUCUUGCCGG 650 1767 CCGGCAAGAGACGCAGUCC 957 1767 GAAUGUCAGCCGAGGCAGG 651 1767 GAAUGUCAGCCGAGGCAGG 651 1785 CCUGCCUCGGCUGACAUUC 958 1785 GGAAUGCGUGGACAAGUGC 652 1785 GGAAUGCGUGGACAAGUGC 652 1803 GCACUUGUCCACGCAUUCC 959 1803 CAAGCUUCUGGAGGGUGAG 653 1803 CAAGCUUCUGGAGGGUGAG 653 1821 CUCACCCUCCAGAAGCUUG 960 1821 GCCAAGGGAGUUUGUGGAG 654 1821 GCCAAGGGAGUUUGUGGAG 654 1839 CUCCACAAACUCCCUUGGC 961 1839 GAACUCUGAGUGCAUACAG 655 1839 GAACUCUGAGUGCAUACAG 655 1857 CUGUAUGCACUCAGAGUUC 962 1857 GUGCCACCCAGAGUGCCUG 656 1857 GUGCCACCCAGAGUGCCUG 656 1875 CAGGCACUCUGGGUGGCAC 963 1875 GCCUCAGGCCAUGAACAUC 657 1875 GCCUCAGGCCAUGAACAUC 657 1893 GAUGUUCAUGGCCUGAGGC 964 1893 CACCUGCACAGGACGGGGA 658 1893 CACCUGCACAGGACGGGGA 658 1911 UCCCCGUCCUGUGCAGGUG 965 1911 ACCAGACAACUGUAUCCAG 659 1911 ACCAGACAACUGUAUCCAG 659 1929 CUGGAUACAGUUGUCUGGU 966 1929 GUGUGCCCACUACAUUGAC 660 1929 GUGUGCCCACUACAUUGAC 660 1947 GUCAAUGUAGUGGGCACAC 967 1947 CGGCCCCCACUGCGUCAAG 661 1947 CGGCCCCCACUGCGUCAAG 661 1965 CUUGACGCAGUGGGGGCCG 968 1965 GACCUGCCCGGCAGGAGUC 662 1965 GACCUGCCCGGCAGGAGUC 662 1983 GACUCCUGCCGGGCAGGUC 969 1983 CAUGGGAGAAAACAACACC 663 1983 CAUGGGAGAAAACAACACC 663 2001 GGUGUUGUUUUCUCCCAUG 970 2001 CCUGGUCUGGAAGUACGCA 664 2001 CCUGGUCUGGAAGUACGCA 664 2019 UGCGUACUUCCAGACCAGG 971 2019 AGACGCCGGCCAUGUGUGC 665 2019 AGACGCCGGCCAUGUGUGC 665 2037 GCACACAUGGCCGGCGUCU 972 2037 CCACCUGUGCCAUCCAAAC 666 2037 CCACCUGUGCCAUCCAAAC 666 2055 GUUUGGAUGGCACAGGUGG 973 2055 CUGCACCUACGGAUGCACU 667 2055 CUGCACCUACGGAUGCACU 667 2073 AGUGCAUCCGUAGGUGCAG 974 2073 UGGGCCAGGUCUUGAAGGC 668 2073 UGGGCCAGGUCUUGAAGGC 668 2091 GCCUUCAAGACCUGGCCCA 975 2091 CUGUCCAACGAAUGGGCCU 669 2091 CUGUCCAACGAAUGGGCCU 669 2109 AGGCCCAUUCGUUGGACAG 976 2109 UAAGAUCCCGUCCAUCGCC 670 2109 UAAGAUCCCGUCCAUCGCC 670 2127 GGCGAUGGACGGGAUCUUA 977 2127 CACUGGGAUGGUGGGGGCC 671 2127 CACUGGGAUGGUGGGGGCC 671 2145 GGCCCCCACCAUCCCAGUG 978 2145 CCUCCUCUUGCUGCUGGUG 672 2145 CCUCCUCUUGCUGCUGGUG 672 2163 CACCAGCAGCAAGAGGAGG 979 2163 GGUGGCCCUGGGGAUCGGC 673 2163 GGUGGCCCUGGGGAUCGGC 673 2181 GCCGAUCCCCAGGGCCACC 980 2181 CCUCUUCAUGCGAAGGCGC 674 2181 CCUCUUCAUGCGAAGGCGC 674 2199 GCGCCUUCGCAUGAAGAGG 981 2199 CCACAUCGUUCGGAAGCGC 675 2199 CCACAUCGUUCGGAAGCGC 675 2217 GCGCUUCCGAACGAUGUGG 982 2217 CACGCUGCGGAGGCUGCUG 676 2217 CACGCUGCGGAGGCUGCUG 676 2235 CAGCAGCCUCCGCAGCGUG 983 2235 GCAGGAGAGGGAGCUUGUG 677 2235 GCAGGAGAGGGAGCUUGUG 677 2253 CACAAGCUCCCUCUCCUGC 984 2253 GGAGCCUCUUACACCCAGU 678 2253 GGAGCCUCUUACACCCAGU 678 2271 ACUGGGUGUAAGAGGCUCC 985 2271 UGGAGAAGCUCCCAACCAA 679 2271 UGGAGAAGCUCCCAACCAA 679 2289 UUGGUUGGGAGCUUCUCCA 986 2289 AGCUCUCUUGAGGAUCUUG 680 2289 AGCUCUCUUGAGGAUCUUG 680 2307 CAAGAUCCUCAAGAGAGCU 987 2307 GAAGGAAACUGAAUUCAAA 681 2307 GAAGGAAACUGAAUUCAAA 681 2325 UUUGAAUUCAGUUUCCUUC 988 2325 AAAGAUCAAAGUGCUGGGC 682 2325 AAAGAUCAAAGUGCUGGGC 682 2343 GCCCAGCACUUUGAUCUUU 989 2343 CUCCGGUGCGUUCGGCACG 683 2343 CUCCGGUGCGUUCGGCACG 683 2361 CGUGCCGAACGCACCGGAG 990 2361 GGUGUAUAAGGGACUCUGG 684 2361 GGUGUAUAAGGGACUCUGG 684 2379 CCAGAGUCCCUUAUACACC 991 2379 GAUCCCAGAAGGUGAGAAA 685 2379 GAUCCCAGAAGGUGAGAAA 685 2397 UUUCUCACCUUCUGGGAUC 992 2397 AGUUAAAAUUCCCGUCGCU 686 2397 AGUUAAAAUUCCCGUCGCU 686 2415 AGCGACGGGAAUUUUAACU 993 2415 UAUCAAGGAAUUAAGAGAA 687 2415 UAUCAAGGAAUUAAGAGAA 687 2433 UUCUCUUAAUUCCUUGAUA 994 2433 AGCAACAUCUCCGAAAGCC 688 2433 AGCAACAUCUCCGAAAGCC 688 2451 GGCUUUCGGAGAUGUUGCU 995 2451 CAACAAGGAAAUCCUCGAU 689 2451 CAACAAGGAAAUCCUCGAU 689 2469 AUCGAGGAUUUCCUUGUUG 996 2469 UGAAGCCUACGUGAUGGCC 690 2469 UGAAGCCUACGUGAUGGCC 690 2487 GGCCAUCACGUAGGCUUCA 997 2487 CAGCGUGGACAACCCCCAC 691 2487 CAGCGUGGACAACCCCCAC 691 2505 GUGGGGGUUGUCCACGCUG 998 2505 CGUGUGCCGCCUGCUGGGC 692 2505 CGUGUGCCGCCUGCUGGGC 692 2523 GCCCAGCAGGCGGCACACG 999 2523 CAUCUGCCUCACCUCCACC 693 2523 CAUCUGCCUCACCUCCACC 693 2541 GGUGGAGGUGAGGCAGAUG 1000 2541 CGUGCAACUCAUCACGCAG 694 2541 CGUGCAACUCAUCACGCAG 694 2559 CUGCGUGAUGAGUUGCACG 1001 2559 GCUCAUGCCCUUCGGCUGC 695 2559 GCUCAUGCCCUUCGGCUGC 695 2577 GCAGCCGAAGGGCAUGAGC 1002 2577 CCUCCUGGACUAUGUCCGG 696 2577 CCUCCUGGACUAUGUCCGG 696 2595 CCGGACAUAGUCCAGGAGG 1003 2595 GGAACACAAAGACAAUAUU 697 2595 GGAACACAAAGACAAUAUU 697 2613 AAUAUUGUCUUUGUGUUCC 1004 2613 UGGCUCCCAGUACCUGCUC 698 2613 UGGCUCCCAGUACCUGCUC 698 2631 GAGCAGGUACUGGGAGCCA 1005 2631 CAACUGGUGUGUGCAGAUC 699 2631 CAACUGGUGUGUGCAGAUC 699 2649 GAUCUGCACACACCAGUUG 1006 2649 CGCAAAGGGCAUGAACUAC 700 2649 CGCAAAGGGCAUGAACUAC 700 2667 GUAGUUCAUGCCCUUUGCG 1007 2667 CUUGGAGGACCGUCGCUUG 701 2667 CUUGGAGGACCGUCGCUUG 701 2685 AGAGCGACGGUCCUCCAAG 1008 2685 GGUGCACCGCGACCUGGCA 702 2685 GGUGCACCGCGACCUGGCA 702 2703 UGCCAGGUCGCGGUGCACC 1009 2703 AGCCAGGAACGUACUGGUG 703 2703 AGCCAGGAACGUACUGGUG 703 2721 CACCAGUACGUUCCUGGCU 1010 2721 GAAAACACCGCAGCAUGUC 704 2721 GAAAACACCGCAGCAUGUC 704 2739 GACAUGCUGCGGUGUUUUC 1011 2739 CAAGAUCACAGAUUUUGGG 705 2739 CAAGAUCACAGAUUUUGGG 705 2757 CCCAAAAUCUGUGAUCUUG 1012 2757 GCUGGCCAAACUGCUGGGU 706 2757 GCUGGCCAAACUGCUGGGU 706 2775 ACCCAGCAGUUUGGCCAGC 1013 2775 UGCGGAAGAGAAAGAAUAC 707 2775 UGCGGAAGAGAAAGAAUAC 707 2793 GUAUUCUUUCUCUUCCGCA 1014 2793 CCAUGCAGAAGGAGGCAAA 708 2793 CCAUGCAGAAGGAGGCAAA 708 2811 UUUGCCUCCUUCUGCAUGG 1015 2811 AGUGCCUAUCAAGUGGAUG 709 2811 AGUGCCUAUCAAGUGGAUG 709 2829 CAUCCACUUGAUAGGCACU 1016 2829 GGCAUUGGAAUCAAUUUUA 710 2829 GGCAUUGGAAUCAAUUUUA 710 2847 UAAAAUUGAUUCCAAUGCC 1017 2847 ACACAGAAUCUAUACCCAC 711 2847 ACACAGAAUCUAUACCCAC 711 2865 GUGGGUAUAGAUUCUGUGU 1018 2865 CCAGAGUGAUGUCUGGAGC 712 2865 CCAGAGUGAUGUCUGGAGC 712 2883 GCUCCAGACAUCACUCUGG 1019 2883 CUACGGGGUGACCGUUUGG 713 2883 CUACGGGGUGACCGUUUGG 713 2901 CCAAACGGUCACCCCGUAG 1020 2901 GGAGUUGAUGACCUUUGGA 714 2901 GGAGUUGAUGACCUUUGGA 714 2919 UCCAAAGGUCAUCAACUCC 1021 2919 AUCCAAGCCAUAUGACGGA 715 2919 AUCCAAGCCAUAUGACGGA 715 2937 UCCGUCAUAUGGCUUGGAU 1022 2937 AAUCCCUGCCAGCGAGAUC 716 2937 AAUCCCUGCCAGCGAGAUC 716 2955 GAUCUCGCUGGCAGGGAUU 1023 2955 CUCCUCCAUCCUGGAGAAA 717 2955 CUCCUCCAUCCUGGAGAAA 717 2973 UUUCUCCAGGAUGGAGGAG 1024 2973 AGGAGAACGCCUCCCUCAG 718 2973 AGGAGAACGCCUCCCUCAG 718 2991 CUGAGGGAGGCGUUCUCCU 1025 2991 GCCACCCAUAUGUACCAUC 719 2991 GCCACCCAUAUGUACCAUC 719 3009 GAUGGUACAUAUGGGUGGC 1026 3009 CGAUGUCUACAUGAUCAUG 720 3009 CGAUGUCUACAUGAUCAUG 720 3027 CAUGAUCAUGUAGACAUCG 1027 3027 GGUCAAGUGCUGGAUGAUA 721 3027 GGUCAAGUGCUGGAUGAUA 721 3045 UAUCAUCCAGCACUUGACC 1028 3045 AGACGCAGAUAGUCGCCCA 722 3045 AGACGCAGAUAGUCGCCCA 722 3063 UGGGCGACUAUCUGCGUCU 1029 3063 AAAGUUCCGUGAGUUGAUC 723 3063 AAAGUUCCGUGAGUUGAUC 723 3081 GAUCAACUCACGGAACUUU 1030 3081 CAUCGAAUUCUCCAAAAUG 724 3081 CAUCGAAUUCUCCAAAAUG 724 3099 CAUUUUGGAGAAUUCGAUG 1031 3099 GGCCCGAGACCCCCAGCGC 725 3099 GGCCCGAGACCCCCAGCGC 725 3117 GCGCUGGGGGUCUCGGGCC 1032 3117 CUACCUUGUCAUUCAGGGG 726 3117 CUACCUUGUCAUUCAGGGG 726 3135 CCCCUGAAUGACAAGGUAG 1033 3135 GGAUGAAAGAAUGCAUUUG 727 3135 GGAUGAAAGAAUGCAUUUG 727 3153 CAAAUGCAUUCUUUCAUCC 1034 3153 GCCAAGUCCUACAGACUCC 728 3153 GCCAAGUCCUACAGACUCC 728 3171 GGAGUCUGUAGGACUUGGC 1035 3171 CAACUUCUACCGUGCCCUG 729 3171 CAACUUCUACCGUGCCCUG 729 3189 CAGGGCACGGUAGAAGUUG 1036 3189 GAUGGAUGAAGAAGACAUG 730 3189 GAUGGAUGAAGAAGACAUG 730 3207 CAUGUCUUCUUCAUCCAUC 1037 3207 GGACGACGUGGUGGAUGCC 731 3207 GGACGACGUGGUGGAUGCC 731 3225 GGCAUCCACCACGUCGUCC 1038 3225 CGACGAGUACCUCAUCCCA 732 3225 CGACGAGUACCUCAUCCCA 732 3243 UGGGAUGAGGUACUCGUCG 1039 3243 ACAGCAGGGCUUCUUCAGC 733 3243 ACAGCAGGGCUUCUUCAGC 733 3261 GCUGAAGAAGCCCUGCUGU 1040 3261 CAGCCCCUCCACGUCACGG 734 3261 CAGCCCCUCCACGUCACGG 734 3279 CCGUGACGUGGAGGGGCUG 1041 3279 GACUCCCCUCCUGAGCUCU 735 3279 GACUCCCCUCCUGAGCUCU 735 3297 AGAGCUCAGGAGGGGAGUC 1042 3297 UCUGAGUGCAACCAGCAAC 736 3297 UCUGAGUGCAACCAGCAAC 736 3315 GUUGCUGGUUGCACUCAGA 1043 3315 CAAUUCCACCGUGGCUUGC 737 3315 CAAUUCCACCGUGGCUUGC 737 3333 GCAAGCCACGGUGGAAUUG 1044 3333 CAUUGAUAGAAAUGGGCUG 738 3333 CAUUGAUAGAAAUGGGCUG 738 3351 CAGCCCAUUUCUAUCAAUG 1045 3351 GCAAAGCUGUCCCAUCAAG 739 3351 GCAAAGCUGUCCCAUCAAG 739 3369 CUUGAUGGGACAGCUUUGC 1046 3369 GGAAGACAGCUUCUUGCAG 740 3369 GGAAGACAGCUUCUUGCAG 740 3387 CUGCAAGAAGCUGUCUUCC 1047 3387 GCGAUACAGCUCAGACCCC 741 3387 GCGAUACAGCUCAGACCCC 741 3405 GGGGUCUGAGCUGUAUCGC 1048 3405 CACAGGCGCCUUGACUGAG 742 3405 CACAGGCGCCUUGACUGAG 742 3423 CUCAGUCAAGGCGCCUGUG 1049 3423 GGACAGCAUAGACGACACC 743 3423 GGACAGCAUAGACGACACC 743 3441 GGUGUCGUCUAUGCUGUCC 1050 3441 CUUCCUCCCAGUGCCUGAA 744 3441 CUUCCUCCCAGUGCCUGAA 744 3459 UUCAGGCACUGGGAGGAAG 1051 3459 AUACAUAAACCAGUCCGUU 745 3459 AUACAUAAACCAGUCCGUU 745 3477 AACGGACUGGUUUAUGUAU 1052 3477 UCCCAAAAGGCCCGCUGGC 746 3477 UCCCAAAAGGCCCGCUGGC 746 3495 GCCAGCGGGCCUUUUGGGA 1053 3495 CUCUGUGCAGAAUCCUGUC 747 3495 CUCUGUGCAGAAUCCUGUC 747 3513 GACAGGAUUCUGCACAGAG 1054 3513 CUAUCACAAUCAGCCUCUG 748 3513 CUAUCACAAUCAGCCUCUG 748 3531 CAGAGGCUGAUUGUGAUAG 1055 3531 GAACCCCGCGCCCAGCAGA 749 3531 GAACCCCGCGCCCAGCAGA 749 3549 UCUGCUGGGCGCGGGGUUC 1056 3549 AGACCCACACUACCAGGAC 750 3549 AGACCCACACUACCAGGAC 750 3567 GUCCUGGUAGUGUGGGUCU 1057 3567 CCCCCACAGCACUGCAGUG 751 3567 CCCCCACAGCACUGCAGUG 751 3585 CACUGCAGUGCUGUGGGGG 1058 3585 GGGCAACCCCGAGUAUCUC 752 3585 GGGCAACCCCGAGUAUCUC 752 3603 GAGAUACUCGGGGUUGCCC 1059 3603 CAACACUGUCCAGCCCACC 753 3603 CAACACUGUCCAGCCCACC 753 3621 GGUGGGCUGGACAGUGUUG 1060 3621 CUGUGUCAACAGCACAUUC 754 3621 CUGUGUCAACAGCACAUUC 754 3639 GAAUGUGCUGUUGACACAG 1061 3639 CGACAGCCCUGCCCACUGG 755 3639 CGACAGCCCUGCCCACUGG 755 3657 CCAGUGGGCAGGGCUGUCG 1062 3657 GGCCCAGAAAGGCAGCCAC 756 3657 GGCCCAGAAAGGCAGCCAC 756 3675 GUGGCUGCCUUUCUGGGCC 1063 3675 CCAAAUUAGCCUGGACAAC 757 3675 CCAAAUUAGCCUGGACAAC 757 3693 GUUGUCCAGGCUAAUUUGG 1064 3693 CCCUGACUACCAGCAGGAC 758 3693 CCCUGACUACCAGCAGGAC 758 3711 GUCCUGCUGGUAGUCAGGG 1065 3711 CUUCUUUCCCAAGGAAGCC 759 3711 CUUCUUUCCCAAGGAAGCC 759 3729 GGCUUCCUUGGGAXAGAAG 1066 3729 CAAGCCAAAUGGCAUCUUU 760 3729 CAAGCCAAAUGGCAUCUUU 760 3747 AAAGAUGCCAUUUGGCUUG 1067 3747 UAAGGGCUCCACAGCUGAA 761 3747 UAAGGGCUCCACAGCUGAA 761 3765 UUCAGCUGUGGAGCCCUUA 1068 3765 AAAUGCAGAAUACCUAAGG 762 3765 AAAUGCAGAAUACCUAAGG 762 3783 CCUUAGGUAUUCUGCAUUU 1069 3783 GGUCGCGCCACAAAGCAGU 763 3783 GGUCGCGCCACAAAGCAGU 763 3801 ACUGCUUUGUGGCGCGACC 1070 3801 UGAAUUUAUUGGAGCAUGA 764 3801 UGAAUUUAUUGGAGCAUGA 764 3819 UCAUGCUCCAAUAAAUUCA 1071 3819 ACCACGGAGGAUAGUAUGA 765 3819 ACCACGGAGGAUAGUAUGA 765 3837 UCAUACUAUCCUCCGUGGU 1072 3837 AGCCCUAAAAAUCCAGACU 766 3837 AGCCCUAAAAAUCCAGACU 766 3855 AGUCUGGAUUUUUAGGGCU 1073 3855 UCUUUCGAUACCCAGGACC 767 3855 UCUUUCGAUACCCAGGACC 767 3873 GGUCCUGGGUAUCGAAAGA 1074 3873 CAAGCCACAGCAGGUCCUC 768 3873 CAAGCCACAGCAGGUCCUC 768 3891 GAGGACCUGCUGUGGCUUG 1075 3891 CCAUCCCAACAGCCAUGCC 769 3891 CCAUCCCAACAGCCAUGCC 769 3909 GGCAUGGCUGUUGGGAUGG 1076 3909 CCGCAUUAGCUCUUAGACC 770 3909 CCGCAUUAGCUCUUAGACC 770 3927 GGUCUAAGAGCUAAUGCGG 1077 3927 CCACAGACUGGUUUUGCAA 771 3927 CCACAGACUGGUUUUGCAA 771 3945 UUGCAAAACCAGUCUGUGG 1078 3945 ACGUUUACACCGACUAGCC 772 3945 ACGUUUACACCGACUAGCC 772 3963 GGCUAGUCGGUGUAAACGU 1079 3963 CAGGAAGUACUUCCACCUC 773 3963 CAGGAAGUACUUCCACCUC 773 3981 GAGGUGGAAGUACUUCCUG 1080 3981 CGGGCACAUUUUGGGAAGU 774 3981 CGGGCACAUUUUGGGAAGU 774 3999 ACUUCCCAAAAUGUGCCCG 1081 3999 UUGCAUUCCUUUGUCUUCA 775 3999 UUGCAUUCCUUUGUCUUCA 775 4017 UGAAGACAAAGGAAUGCAA 1082 4017 AAACUGUGAAGCAUUUACA 776 4017 AAACUGUGAAGCAUUUACA 776 4035 UGUAAAUGCUUCACAGUUU 1083 4035 AGAAACGCAUCCAGCAAGA 777 4035 AGAAACGCAUCCAGCAAGA 777 4053 UCUUGCUGGAUGCGUUUCU 1084 4053 AAUAUUGUCCCUUUGAGCA 778 4053 AAUAUUGUCCCUUUGAGCA 778 4071 UGCUCAAAGGGACAAUAUU 1085 4071 AGAAAUUUAUCUUUCAAAG 779 4071 AGAAAUUUAUCUUUCAAAG 779 4089 CUUUGAAAGAUAAAUUUCU 1086 4089 GAGGUAUAUUUGAAAAAAA 780 4089 GAGGUAUAUUUGAAAAAAA 780 4107 UUUUUUUCAAAUAUACCUC 1087 4107 AAAAAAAAAGUAUAUGUGA 781 4107 AAAAAAAAAGUAUAUGUGA 781 4125 UCACAUAUACUUUUUUUUU 1088 4125 AGGAUUUUUAUUGAUUGGG 782 4125 AGGAUUUUUAUUGAUUGGG 782 4143 CCCAAUCAAUAAAAAUCCU 1089 4143 GGAUCUUGGAGUUUUUCAU 783 4143 GGAUCUUGGAGUUUUUCAU 783 4161 AUGAAAAACUCCAAGAUCC 1090 4161 UUGUCGCUAUUGAUUUUUA 784 4161 UUGUCGCUAUUGAUUUUUA 784 4179 UAAAAAUCAAUAGCGACAA 1091 4179 ACUUCAAUGGGCUCUUCCA 785 4179 ACUUCAAUGGGCUCUUCCA 785 4197 UGGAAGAGCCCAUUGAAGU 1092 4197 AACAAGGAAGAAGCUUGCU 786 4197 AACAAGGAAGAAGCUUGCU 786 4215 AGCAAGCUUCUUCCUUGUU 1093 4215 UGGUAGCACUUGCUACCCU 787 4215 UGGUAGCACUUGCUACCCU 787 4233 AGGGUAGCAAGUGCUACCA 1094 4233 UGAGUUCAUCCAGGCCCAA 788 4233 UGAGUUCAUCCAGGCCCAA 788 4251 UUGGGCCUGGAUGAACUCA 1095 4251 ACUGUGAGCAAGGAGCACA 789 4251 ACUGUGAGCAAGGAGCACA 789 4269 UGUGCUCCUUGCUCACAGU 1096 4269 AAGCCACAAGUCUUCCAGA 790 4269 AAGCCACAAGUCUUCCAGA 790 4287 UCUGGAAGACUUGUGGCUU 1097 4287 AGGAUGCUUGAUUCCAGUG 791 4287 AGGAUGCUUGAUUCCAGUG 791 4305 CACUGGAAUCAAGCAUCCU 1098 4305 GGUUCUGCUUCAAGGCUUC 792 4305 GGUUCUGCUUCAAGGCUUC 792 4323 GAAGCCUUGAAGCAGAACC 1099 4323 CCACUGCAAAACACUAAAG 793 4323 CCACUGCAAAACACUAAAG 793 4341 CUUUAGUGUUUUGCAGUGG 1100 4341 GAUCCAAGAAGGCCUUCAU 794 4341 GAUCCAAGAAGGCCUUCAU 794 4359 AUGAAGGCCUUCUUGGAUC 1101 4359 UGGCCCCAGCAGGCCGGAU 795 4359 UGGCCCCAGCAGGCCGGAU 795 4377 AUCCGGCCUGCUGGGGCCA 1102 4377 UCGGUACUGUAUCAAGUCA 796 4377 UCGGUACUGUAUCAAGUCA 796 4395 UGACUUGAUACAGUACCGA 1103 4395 AUGGCAGGUACAGUAGGAU 797 4395 AUGGCAGGUACAGUAGGAU 797 4413 AUCCUACUGUACCUGCCAU 1104 4413 UAAGCCACUCUGUCCCUUC 798 4413 UAAGCCACUCUGUCCCUUC 798 4431 GAAGGGACAGAGUGGCUUA 1105 4431 CCUGGGCAAAGAAGAAACG 799 4431 CCUGGGCAAAGAAGAAACG 799 4449 CGUUUCUUCUUUGCCCAGG 1106 4449 GGAGGGGAUGAAUUCUUCC 800 4449 GGAGGGGAUGAAUUCUUCC 800 4467 GGAAGAAUUCAUCCCCUCC 1107 4467 CUUAGACUUACUUUUGUAA 801 4467 CUUAGACUUACUUUUGUAA 801 4485 UUACAAAAGUAAGUCUAAG 1108 4485 AAAAUGUCCCCACGGUACU 802 4485 AAAAUGUCCCCACGGUACU 802 4503 AGUACCGUGGGGACAUUUU 1109 4503 UUACUCCCCACUGAUGGAC 803 4503 UUACUCCCCACUGAUGGAC 803 4521 GUCCAUCAGUGGGGAGUAA 1110 4521 CCAGUGGUUUCCAGUCAUG 804 4521 CCAGUGGUUUCCAGUCAUG 804 4539 CAUGACUGGAAACCACUGG 1111 4539 GAGCGUUAGACUGACUUGU 805 4539 GAGCGUUAGACUGACUUGU 805 4557 ACAAGUCAGUCUAACGCUC 1112 4557 UUUGUCUUCCAUUCCAUUG 806 4557 UUUGUCUUCCAUUCCAUUG 806 4575 CAAUGGAAUGGAAGACAAA 1113 4575 GUUUUGAAACUCAGUAUGC 807 4575 GUUUUGAAACUCAGUAUGC 807 4593 GCAUACUGAGUUUCAAAAC 1114 4593 CCGCCCCUGUCUUGCUGUC 808 4593 CCGCCCCUGUCUUGCUGUC 808 4611 GACAGCAAGACAGGGGCGG 1115 4611 CAUGAAAUCAGCAAGAGAG 809 4611 CAUGAAAUCAGCAAGAGAG 809 4629 CUCUCUUGCUGAUUUCAUG 1116 4629 GGAUGACACAUCAAAUAAU 810 4629 GGAUGACACAUCAAAUAAU 810 4647 AUUAUUUGAUGUGUCAUCC 1117 4647 UAACUCGGAUUCCAGCCCA 811 4647 UAACUCGGAUUCCAGCCCA 811 4665 UGGGCUGGAAUCCGAGUUA 1118 4665 ACAUUGGAUUCAUCAGCAU 812 4665 ACAUUGGAUUCAUCAGCAU 812 4683 AUGCUGAUGAAUCCAAUGU 1119 4683 UUUGGACCAAUAGCCCACA 813 4683 UUUGGACCAAUAGCCCACA 813 4701 UGUGGGCUAUUGGUCCAAA 1120 4701 AGCUGAGAAUGUGGAAUAC 814 4701 AGCUGAGAAUGUGGAAUAC 814 4719 GUAUUCCACAUUCUCAGCU 1121 4719 CCUAAGGAUAACACCGCUU 815 4719 CCUAAGGAUAACACCGCUU 815 4737 AAGCGGUGUUAUCCUUAGG 1122 4737 UUUGUUCUCGCAAAAACGU 816 4737 UUUGUUCUCGCAAAAACGU 816 4755 ACGUUUUUGCGAGAACAAA 1123 4755 UAUCUCCUAAUUUGAGGCU 817 4755 UAUCUCCUAAUUUGAGGCU 817 4773 AGCCUCAAAUUAGGAGAUA 1124 4773 UCAGAUGAAAUGCAUCAGG 818 4773 UCAGAUGAAAUGCAUCAGG 818 4791 CCUGAUGCAUUUCAUCUGA 1125 4791 GUCCUUUGGGGCAUAGAUC 819 4791 GUCCUUUGGGGCAUAGAUC 819 4809 GAUCUAUGCCCCAAAGGAC 1126 4809 CAGAAGACUACAAAAAUGA 820 4809 CAGAAGACUACAAAAAUGA 820 4827 UCAUUUUUGUAGUCUUCUG 1127 4827 AAGCUGCUCUGAAAUCUCC 821 4827 AAGCUGCUCUGAAAUCUCC 821 4845 GGAGAUUUCAGAGCAGCUU 1128 4845 CUUUAGCCAUCACCCCAAC 822 4845 CUUUAGCCAUCACCCCAAC 822 4863 GUUGGGGUGAUGGCUAAAG 1129 4863 CCCCCCAAAAUUAGUUUGU 823 4863 CCCCCCAAAAUUAGUUUGU 823 4881 ACAAACUAAUUUUGGGGGG 1130 4881 UGUUACUUAUGGXAGAUAG 824 4881 UGUUACUUAUGGAAGAUAG 824 4899 CUAUCUUCCAUAAGUAACA 1131 4899 GUUUUCUCCUUUUACUUCA 825 4899 GUUUUCUCCUUUUACUUCA 825 4917 UGAAGUAAAAGGAGAAAAC 1132 4917 ACUUCAAAAGCUUUUUACU 826 4917 ACUUCAAAAGCUUUUUACU 826 4935 AGUAAAAAGCUUUUGAAGU 1133 4935 UCAAAGAGUAUAUGUUCCC 827 4935 UCAAAGAGUAUAUGUUCCC 827 4953 GGGAACAUAUACUCUUUGA 1134 4953 CUCCAGGUCAGCUGCCCCC 828 4953 CUCCAGGUCAGCUGCCCCC 828 4971 GGGGGCAGCUGACCUGGAG 1135 4971 CAAACCCCCUCCUUACGCU 829 4971 CAAACCCCCUCCUUACGCU 829 4989 AGCGUAAGGAGGGGGUUUG 1136 4989 UUUGUCACACAAAAAGUGU 830 4989 UUUGUCACACAAAAAGUGU 830 5007 ACACUUUUUGUGUGACAAA 1137 5007 UCUCUGCCUUGAGUCAUCU 831 5007 UCUCUGCCUUGAGUCAUCU 831 5025 AGAUGACUCAAGGCAGAGA 1138 5025 UAUUCAAGCACUUACAGCU 832 5025 UAUUCAAGCACUUACAGCU 832 5043 AGCUGUAAGUGCUUGAAUA 1139 5043 UCUGGCCACAACAGGGCAU 833 5043 UCUGGCCACAACAGGGCAU 833 5061 AUGCCCUGUUGUGGCCAGA 1140 5061 UUUUACAGGUGCGAAUGAC 834 5061 UUUUACAGGUGCGAAUGAC 834 5079 GUCAUUCGCACCUGUAAAA 1141 5079 CAGUAGCAUUAUGAGUAGU 835 5079 CAGUAGCAUUAUGAGUAGU 835 5097 ACUACUCAUAAUGCUACUG 1142 5097 UGUGAAUUCAGGUAGUAAA 836 5097 UGUGAAUUCAGGUAGUAAA 836 5115 UUUACUACCUGAAUUCACA 1143 5115 AUAUGAAACUAGGGUUUGA 837 5115 AUAUGAAACUAGGGUUUGA 837 5133 UOAAACCCUAGUUUCAUAU 1144 5133 AAAUUGAUAAUGCUUUCAC 838 5133 AAAUUGAUAAUGCUUUCAC 838 5151 GUGAAAGCAUUAUCAAUUU 1145 5151 CAACAUUUGCAGAUGUUUU 839 5151 CAACAUUUGCAGAUGUUUU 839 5169 AAAACAUCUGCAAAUGUUG 1146 5169 UAGAAGGAAAAAAGUUCCU 840 5169 UAGAAGGAAAAAAGUUCCU 840 5187 AGGAACUUUUUUCCUUCUA 1147 5187 UUCCUAAAAUAAUUUCUCU 841 5187 UUCCUAAAAUAAUUUCUCU 841 5205 AGAGAAAUUAUUUUAGGAA 1148 5205 UACAAUUGGAAGAUUGGAA 842 5205 UACAAUUGGAAGAUUGGAA 842 5223 UUCCAAUCUUCCAAUUGUA 1149 5223 AGAUUCAGCUAGUUAGGAG 843 5223 AGAUUCAGCUAGUUAGGAG 843 5241 CUCCUAACUAGCUGAAUCU 1150 5241 GCCCAUUUUUUCCUAAUCU 844 5241 GCCCAUUUUUUCCUAAUCU 844 5259 AGAUUAGGAAAAAAUGGGC 1151 5259 UGUGUGUGCCCUGUAACCU 845 5259 UGUGUGUGCCCUGUAACCU 845 5277 AGGUUACAGGGCACACACA 1152 5277 UGACUGGUUAACAGCAGUC 846 5277 UGACUGGUUAACAGCAGUC 846 5295 GACUGCUGUUAACOAGUCA 1153 5295 CCUUUGUAAACAGUGUUUU 847 5295 CCUUUGUAAACAGUGUUUU 847 5313 AAAACACUGUUUACAAAGG 1154 5313 UAAACUCUCCUAGUCAAUA 848 5313 UAAACUCUCCUAGUCAAUA 848 5331 UAUUGACUAGGAGAGUUUA 1155 5331 AUCCACCCCAUCCAAUUUA 849 5331 AUCCACCCCAUCCAAUUUA 849 5349 UAAAUUGGAUGGGGUGGAU 1156 5349 AUCAAGGAAGAAAUGGUUC 850 5349 AUCAAGGAAGAAAUGGUUC 850 5367 GAACCAUUUCUUCCUUGAU 1157 5367 CAGAAAAUAUUUUCAGCCU 851 5367 CAGAAAAUAUUUUCAGCCU 851 5385 AGGCUGAAAAUAUUUUCUG 1158 5385 UACAGUUAUGUUCAGUCAC 852 5385 UACAGUUAUGUUCAGUCAC 852 5403 GUGACUGAAOAUAACUGUA 1159 5403 CACACACAUACAAAAUGUU 853 5403 CACACACAUACAAAAUGUU 853 5421 AACAUUUUGUAUGUGUGUG 1160 5421 UCCUUUUGCUUUUAAAGUA 854 5421 UCCUUUUGCUUUUAAAGUA 854 5439 UACUUUAAAAGCAAAAGGA 1161 5439 AAUUUUUGACUCCCAGAUC 855 5439 AAUUUUUGACUCCCAGAUC 855 5457 GAUCUGGGAGUCAAAAAUU 1162 5457 CAGUCAGAGCCCCUACAGC 856 5457 CAGUCAGAGCCCCUACAGC 856 5475 GCUGUAGGGGCUCUGACUG 1163 5475 CAUUGUUAAGAAAGUAUUU 857 5475 CAUUGUUAAGAAAGUAUUU 857 5493 AAAUACUUUCUUAACAAUG 1164 5493 UGAUUUUUGUCUCAAUGAA 858 5493 UGAUUUUUGUCUCAAUGAA 858 5511 UUCAUUGAGACAAAAAUCA 1165 5511 AAAUAAAACUAUAUUCAUU 859 5511 AAAUAAAACUAUAUUCAUU 859 5529 AAUGAAUAUAGUUUUAUUU 1166

[0315] TABLE V EGFR (HER1) Synthetic Sequences with all RNA counterparts and Target Sequences Target Seq Pos Target ID Aliases Sequence Seq ID 799 UUGGUCAGUUUCUGGCAGUUCUC 1167 EGFR:801U21 siNA GAACUGCCAGAAACUGACCAA 1171 1380 GCAAAAACCCUGUGAUUUCCUUU 1168 EGFR:1382U21 sINA AGGAAAUCACAGGGUUUUUGC 1172 3064 UCGAUGAUCAACUCACGGAACUU 1169 EGFR:3066U21 siNA GUUCCGUGAGUUGAUCAUCGA 1173 3152 GUUGGAGUCUGUAGGACUUGGCA 1170 EGFR:3154U21 siNA CCAAGUCCUACAGACUCCAAC 1174 799 UUGGUCAGUUUCUGGCAGUUCUC 1167 EGFR:819L21 siNA (801C) GGUCAGUUUCUGGCAGUUCUC 1175 1380 GCAAAAACCCUGUGAUUUCCUUU 1168 EGFR:1400L21 siNA (1382C) AAMACCCUGUGAUUUCCUUU 1176 3064 UCGAUGAUCAACUCACGGAACUU 1169 EGFR:3084L21 siNA (3066C) GAUGAUCAACUCACGGAACUU 1177 3152 GUUGGAGUCUGUAGGACUUGGCA 1170 EGFR:3172L21 sINA (3154C) UGGAGUCUGUAGGACUUGGCA 1178 799 UUGGUCAGUUUCUGGCAGUUCUC 1167 EGFR:801U21 siNAstab4 B GAAcuGccAGAAACuGAccAA B 1179 1380 GCAAAAACCCUGUGAUUUCCUUU 1168 EGFR:1382U21 siNAstab4 B AGGAAAucAcAGGGuuuuuGC B 1180 3064 UCGAUGAUCAACUCACGGAACUU 1169 EGFR:3066U21 siNAstab4 B GuuccGuGAGuuGAucAucGA B 1181 3152 GUUGGAGUCUGUAGGACUUGGCA 1170 EGFR:3154U21 siNAstab4 B ccAAGuccuAcAGAcuccAAc B 1182 799 UUGGUCAGUUUCUGGCAGUUCUC 1167 EGFR:819L21 siNA (801C) stab5 GgucAGuuucuGGcAGuucTsT L 1183 1380 GCAAAAACCCUGUGAUUUCCUUU 1168 EGFR:1400L21 siNA (1382C) stab5 AAAAAcccuGuGAuuuccuTsT L 1184 3064 UCGAUGAUCAACUCACGGAACUU 1169 EGFR:3084L21 siNA (3066C) stab5 GAuGAucAAcucAcGGAAcTsT L 1185 3152 GUUGGAGUCUGUAGGACUUGGCA 1170 EGFR:3172121 siNA (3154C) stab5 uGGAGucuGuAGGAcuuGGTsT L 1186

[0316] TABLE VI A. 2.5 μmol Synthesis Cycle ABI 394 Instrument Wait Time* Wait Time* Wait Time* Reagent Equivalents Amount DNA 2′-O-methyl RNA Phosphoramidites 6.5 163 μL 45 sec 2.5 min 7.5 min S-Ethyl Tetrazole 23.8 238 μL 45 sec 2.5 min 7.5 min Acetic Anhydride 100 233 μL 5 sec 5 sec 5 sec N-Methyl 186 233 μL 5 sec 5 sec 5 sec Imidazole TCA 176 2.3 mL 21 sec 21 sec 21 sec Iodine 11.2 1.7 mL 45 sec 45 sec 45 sec Beaucage 12.9 645 μL 100 sec 300 sec 300 sec Acetonitrile NA 6.67 mL NA NA NA B. 0.2 μmol Synthesis Cycle ABI 394 Instrument Wait Time* Wait Time* Wait Time* Reagent Equivalents Amount DNA 2′-O-methyl RNA Phosphoramidites 15 31 μL 45 sec 233 sec 465 sec S-Ethyl Tetrazole 38.7 31 μL 45 sec 233 min 465 sec Acetic Anhydride 655 124 μL 5 sec 5 sec 5 sec N-Methyl 1245 124 μL 5 sec 5 sec 5 sec Imidazole TCA 700 732 μL 10 sec 10 sec 10 sec Iodine 20.6 244 μL 15 sec 15 sec 15 sec Beaucage 7.7 232 μL 100 sec 300 sec 300 sec Acetonitrile NA 2.64 mL NA NA NA C. 0.2 μmol Synthesis Cycle 96 well Instrument Equivalents: DNA/ Amount: DNA/ Wait Time* Wait Time* Wait Time* Reagent 2′-O-methyl/Ribo 2′-O-methyl/Ribo DNA 2′-O-methyl Ribo Phosphoramidites 22/33/66 40/60/120 μL 60 sec 180 sec 360 sec S-Ethyl Tetrazole 70/105/210 40/60/120 μL 60 sec 180 min 360 sec Acetic Anhydride 265/265/265 50/50/50 μL 10 sec 10 sec 10 sec N-Methyl 502/502/502 50/50/50 μL 10 sec 10 sec 10 sec Imidazole TCA 238/475/475 250/500/500 μL 15 sec 15 sec 15 sec Iodine 6.8/6.8/6.8 80/80/80 μL 30 sec 30 sec 30 sec Beaucage 34/51/51 80/120/120 100 sec 200 sec 200 sec Acetonitrile NA 1150/1150/1150 μL NA NA NA

[0317]

1 1213 1 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 1 aaggggaggu aacccuggc 19 2 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 2 ccccuuuggu cggggcccc 19 3 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 3 cgggcagccg cgcgccccu 19 4 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 4 uucccacggg gcccuuuac 19 5 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 5 cugcgccgcg cgcccggcc 19 6 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 6 ccccaccccu cgcagcacc 19 7 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 7 cccgcgcccc gcgcccucc 19 8 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 8 ccagccgggu ccagccgga 19 9 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 9 agccaugggg ccggagccg 19 10 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 10 gcagugagca ccauggagc 19 11 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 11 cuggcggccu ugugccgcu 19 12 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 12 ugggggcucc uccucgccc 19 13 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 13 cucuugcccc ccggagccg 19 14 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 14 gcgagcaccc aagugugca 19 15 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 15 accggcacag acaugaagc 19 16 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 16 cugcggcucc cugccaguc 19 17 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 17 cccgagaccc accuggaca 19 18 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 18 augcuccgcc accucuacc 19 19 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 19 cagggcugcc agguggugc 19 20 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 20 cagggaaacc uggaacuca 19 21 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 21 accuaccugc ccaccaaug 19 22 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 22 gccagccugu ccuuccugc 19 23 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 23 caggauaucc aggaggugc 19 24 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 24 cagggcuacg ugcucaucg 19 25 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 25 gcucacaacc aagugaggc 19 26 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 26 caggucccac ugcagaggc 19 27 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 27 cugcggauug ugcgaggca 19 28 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 28 acccagcucu uugaggaca 19 29 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 29 aacuaugccc uggccgugc 19 30 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 30 cuagacaaug gagacccgc 19 31 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 31 cugaacaaua ccaccccug 19 32 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 32 gucacagggg ccuccccag 19 33 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 33 ggaggccugc gggagcugc 19 34 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 34 cagcuucgaa gccucacag 19 35 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 35 gagaucuuga aaggagggg 19 36 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 36 gucuugaucc agcggaacc 19 37 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 37 ccccagcucu gcuaccagg 19 38 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 38 gacacgauuu uguggaagg 19 39 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 39 gacaucuucc acaagaaca 19 40 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 40 aaccagcugg cucucacac 19 41 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 41 cugauagaca ccaaccgcu 19 42 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 42 ucucgggccu gccaccccu 19 43 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 43 uguucuccga uguguaagg 19 44 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 44 ggcucccgcu gcuggggag 19 45 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 45 gagaguucug aggauuguc 19 46 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 46 cagagccuga cgcgcacug 19 47 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 47 gucugugccg guggcugug 19 48 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 48 gcccgcugca aggggccac 19 49 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 49 cugcccacug acugcugcc 19 50 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 50 caugagcagu gugcugccg 19 51 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 51 ggcugcacgg gccccaagc 19 52 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 52 cacucugacu gccuggccu 19 53 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 53 ugccuccacu ucaaccaca 19 54 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 54 aguggcaucu gugagcugc 19 55 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 55 cacugcccag cccugguca 19 56 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 56 accuacaaca cagacacgu 19 57 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 57 uuugagucca ugcccaauc 19 58 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 58 cccgagggcc gguauacau 19 59 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 59 uucggcgcca gcuguguga 19 60 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 60 acugccuguc ccuacaacu 19 61 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 61 uaccuuucua cggacgugg 19 62 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 62 ggauccugca cccucgucu 19 63 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 63 ugcccccugc acaaccaag 19 64 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 64 gaggugacag cagaggaug 19 65 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 65 ggaacacagc ggugugaga 19 66 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 66 aagugcagca agcccugug 19 67 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 67 gcccgagugu gcuaugguc 19 68 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 68 cugggcaugg agcacuugc 19 69 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 69 cgagagguga gggcaguua 19 70 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 70 accagugcca auauccagg 19 71 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 71 gaguuugcug gcugcaaga 19 72 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 72 aagaucuuug ggagccugg 19 73 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 73 gcauuucugc cggagagcu 19 74 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 74 uuugaugggg acccagccu 19 75 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 75 uccaacacug ccccgcucc 19 76 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 76 cagccagagc agcuccaag 19 77 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 77 guguuugaga cucuggaag 19 78 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 78 gagaucacag guuaccuau 19 79 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 79 uacaucucag cauggccgg 19 80 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 80 gacagccugc cugaccuca 19 81 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 81 agcgucuucc agaaccugc 19 82 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 82 caaguaaucc ggggacgaa 19 83 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 83 auucugcaca auggcgccu 19 84 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 84 uacucgcuga cccugcaag 19 85 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 85 gggcugggca ucagcuggc 19 86 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 86 cuggggcugc gcucacuga 19 87 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 87 agggaacugg gcaguggac 19 88 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 88 cuggcccuca uccaccaua 19 89 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 89 aacacccacc ucugcuucg 19 90 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 90 gugcacacgg ugcccuggg 19 91 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 91 gaccagcucu uucggaacc 19 92 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 92 ccgcaccaag cucugcucc 19 93 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 93 cacacugcca accggccag 19 94 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 94 gaggacgagu gugugggcg 19 95 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 95 gagggccugg ccugccacc 19 96 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 96 cagcugugcg cccgagggc 19 97 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 97 cacugcuggg guccagggc 19 98 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 98 cccacccagu gugucaacu 19 99 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 99 ugcagccagu uccuucggg 19 100 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 100 ggccaggagu gcguggagg 19 101 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 101 gaaugccgag uacugcagg 19 102 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 102 gggcucccca gggaguaug 19 103 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 103 gugaaugcca ggcacuguu 19 104 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 104 uugccgugcc acccugagu 19 105 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 105 ugucagcccc agaauggcu 19 106 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 106 ucagugaccu guuuuggac 19 107 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 107 ccggaggcug accagugug 19 108 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 108 guggccugug cccacuaua 19 109 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 109 aaggacccuc ccuucugcg 19 110 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 110 guggcccgcu gccccagcg 19 111 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 111 ggugugaaac cugaccucu 19 112 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 112 uccuacaugc ccaucugga 19 113 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 113 aaguuuccag augaggagg 19 114 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 114 ggcgcaugcc agccuugcc 19 115 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 115 cccaucaacu gcacccacu 19 116 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 116 uccugugugg accuggaug 19 117 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 117 gacaagggcu gccccgccg 19 118 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 118 gagcagagag ccagcccuc 19 119 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 119 cugacgucca ucaucucug 19 120 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 120 gcggugguug gcauucugc 19 121 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 121 cuggucgugg ucuuggggg 19 122 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 122 guggucuuug ggauccuca 19 123 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 123 aucaagcgac ggcagcaga 19 124 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 124 aagauccgga aguacacga 19 125 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 125 augcggagac ugcugcagg 19 126 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 126 gaaacggagc ugguggagc 19 127 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 127 ccgcugacac cuagcggag 19 128 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 128 gcgaugccca accaggcgc 19 129 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 129 cagaugcgga uccugaaag 19 130 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 130 gagacggagc ugaggaagg 19 131 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 131 gugaaggugc uuggaucug 19 132 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 132 ggcgcuuuug gcacagucu 19 133 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 133 uacaagggca ucuggaucc 19 134 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 134 ccugaugggg agaauguga 19 135 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 135 aaaauuccag uggccauca 19 136 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 136 aaaguguuga gggaaaaca 19 137 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 137 acauccccca aagccaaca 19 138 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 138 aaagaaaucu uagacgaag 19 139 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 139 gcauacguga uggcuggug 19 140 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 140 gugggcuccc cauaugucu 19 141 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 141 ucccgccuuc ugggcaucu 19 142 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 142 ugccugacau ccacggugc 19 143 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 143 cagcugguga cacagcuua 19 144 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 144 augcccuaug gcugccucu 19 145 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 145 uuagaccaug uccgggaaa 19 146 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 146 aaccgcggac gccugggcu 19 147 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 147 ucccaggacc ugcugaacu 19 148 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 148 ugguguaugc agauugcca 19 149 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 149 aaggggauga gcuaccugg 19 150 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 150 gaggaugugc ggcucguac 19 151 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 151 cacagggacu uggccgcuc 19 152 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 152 cggaacgugc uggucaaga 19 153 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 153 agucccaacc augucaaaa 19 154 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 154 auuacagacu ucgggcugg 19 155 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 155 gcucggcugc uggacauug 19 156 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 156 gacgagacag aguaccaug 19 157 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 157 gcagaugggg gcaaggugc 19 158 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 158 cccaucaagu ggauggcgc 19 159 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 159 cuggagucca uucuccgcc 19 160 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 160 cggcgguuca cccaccaga 19 161 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 161 agugaugugu ggaguuaug 19 162 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 162 ggugugacug ugugggagc 19 163 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 163 cugaugacuu uuggggcca 19 164 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 164 aaaccuuacg augggaucc 19 165 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 165 ccagcccggg agaucccug 19 166 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 166 gaccugcugg aaaaggggg 19 167 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 167 gagcggcugc cccagcccc 19 168 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 168 cccaucugca ccauugaug 19 169 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 169 gucuacauga ucaugguca 19 170 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 170 aaauguugga ugauugacu 19 171 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 171 ucugaauguc ggccaagau 19 172 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 172 uuccgggagu uggugucug 19 173 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 173 gaauucuccc gcauggcca 19 174 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 174 agggaccccc agcgcuuug 19 175 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 175 guggucaucc agaaugagg 19 176 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 176 gacuugggcc cagccaguc 19 177 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 177 cccuuggaca gcaccuucu 19 178 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 178 uaccgcucac ugcuggagg 19 179 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 179 gacgaugaca ugggggacc 19 180 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 180 cugguggaug cugaggagu 19 181 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 181 uaucugguac cccagcagg 19 182 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 182 ggcuucuucu guccagacc 19 183 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 183 ccugccccgg gcgcugggg 19 184 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 184 ggcauggucc accacaggc 19 185 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 185 caccgcagcu caucuacca 19 186 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 186 aggaguggcg guggggacc 19 187 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 187 cugacacuag ggcuggagc 19 188 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 188 cccucugaag aggaggccc 19 189 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 189 cccaggucuc cacuggcac 19 190 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 190 cccuccgaag gggcuggcu 19 191 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 191 uccgauguau uugauggug 19 192 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 192 gaccugggaa ugggggcag 19 193 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 193 gccaaggggc ugcaaagcc 19 194 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 194 cuccccacac augacccca 19 195 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 195 agcccucuac agcgguaca 19 196 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 196 agugaggacc ccacaguac 19 197 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 197 ccccugcccu cugagacug 19 198 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 198 gauggcuacg uugcccccc 19 199 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 199 cugaccugca gcccccagc 19 200 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 200 ccugaauaug ugaaccagc 19 201 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 201 ccagauguuc ggccccagc 19 202 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 202 cccccuucgc cccgagagg 19 203 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 203 ggcccucugc cugcugccc 19 204 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 204 cgaccugcug gugccacuc 19 205 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 205 cuggaaaggc ccaagacuc 19 206 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 206 cucuccccag ggaagaaug 19 207 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 207 ggggucguca aagacguuu 19 208 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 208 uuugccuuug ggggugccg 19 209 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 209 guggagaacc ccgaguacu 19 210 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 210 uugacacccc agggaggag 19 211 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 211 gcugccccuc agccccacc 19 212 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 212 ccuccuccug ccuucagcc 19 213 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 213 ccagccuucg acaaccucu 19 214 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 214 uauuacuggg accaggacc 19 215 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 215 ccaccagagc ggggggcuc 19 216 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 216 ccacccagca ccuucaaag 19 217 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 217 gggacaccua cggcagaga 19 218 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 218 aacccagagu accuggguc 19 219 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 219 cuggacgugc cagugugaa 19 220 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 220 accagaaggc caaguccgc 19 221 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 221 cagaagcccu gaugugucc 19 222 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 222 cucagggagc agggaaggc 19 223 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 223 ccugacuucu gcuggcauc 19 224 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 224 caagaggugg gagggcccu 19 225 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 225 uccgaccacu uccagggga 19 226 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 226 aaccugccau gccaggaac 19 227 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 227 ccuguccuaa ggaaccuuc 19 228 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 228 ccuuccugcu ugaguuccc 19 229 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 229 cagauggcug gaagggguc 19 230 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 230 ccagccucgu uggaagagg 19 231 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 231 gaacagcacu ggggagucu 19 232 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 232 uuuguggauu cugaggccc 19 233 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 233 cugcccaaug agacucuag 19 234 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 234 ggguccagug gaugccaca 19 235 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 235 agcccagcuu ggcccuuuc 19 236 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 236 ccuuccagau ccuggguac 19 237 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 237 cugaaagccu uagggaagc 19 238 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 238 cuggccugag aggggaagc 19 239 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 239 cggcccuaag ggagugucu 19 240 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 240 uaagaacaaa agcgaccca 19 241 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 241 auucagagac ugucccuga 19 242 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 242 aaaccuagua cugcccccc 19 243 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 243 caugaggaag gaacagcaa 19 244 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 244 auggugucag uauccaggc 19 245 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 245 cuuuguacag agugcuuuu 19 246 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 246 ucuguuuagu uuuuacuuu 19 247 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 247 uuuuuguuuu guuuuuuua 19 248 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 248 aaagaugaaa uaaagaccc 19 249 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 249 aauaaagacc cagggggag 19 250 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 250 gccaggguua ccuccccuu 19 251 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 251 ggggccccga ccaaagggg 19 252 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 252 aggggcgcgc ggcugcccg 19 253 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 253 guaaagggcc ccgugggaa 19 254 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 254 ggccgggcgc gcggcgcag 19 255 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 255 ggugcugcga ggggugggg 19 256 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 256 ggagggcgcg gggcgcggg 19 257 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 257 uccggcugga cccggcugg 19 258 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 258 cggcuccggc cccauggcu 19 259 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 259 gcuccauggu gcucacugc 19 260 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 260 agcggcacaa ggccgccag 19 261 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 261 gggcgaggag gagccccca 19 262 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 262 cggcuccggg gggcaagag 19 263 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 263 ugcacacuug ggugcucgc 19 264 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 264 gcuucauguc ugugccggu 19 265 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 265 gacuggcagg gagccgcag 19 266 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 266 uguccaggug ggucucggg 19 267 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 267 gguagaggug gcggagcau 19 268 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 268 gcaccaccug gcagcccug 19 269 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 269 ugaguuccag guuucccug 19 270 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 270 cauugguggg cagguaggu 19 271 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 271 gcaggaagga caggcuggc 19 272 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 272 gcaccuccug gauauccug 19 273 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 273 cgaugagcac guagcccug 19 274 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 274 gccucacuug guugugagc 19 275 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 275 gccucugcag ugggaccug 19 276 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 276 ugccucgcac aauccgcag 19 277 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 277 uguccucaaa gagcugggu 19 278 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 278 gcacggccag ggcauaguu 19 279 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 279 gcgggucucc auugucuag 19 280 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 280 cagggguggu auuguucag 19 281 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 281 cuggggaggc cccugugac 19 282 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 282 gcagcucccg caggccucc 19 283 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 283 cugugaggcu ucgaagcug 19 284 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 284 ccccuccuuu caagaucuc 19 285 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 285 gguuccgcug gaucaagac 19 286 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 286 ccugguagca gagcugggg 19 287 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 287 ccuuccacaa aaucguguc 19 288 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 288 uguucuugug gaagauguc 19 289 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 289 gugugagagc cagcugguu 19 290 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 290 agcgguuggu gucuaucag 19 291 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 291 agggguggca ggcccgaga 19 292 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 292 ccuuacacau cggagaaca 19 293 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 293 cuccccagca gcgggagcc 19 294 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 294 gacaauccuc agaacucuc 19 295 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 295 cagugcgcgu caggcucug 19 296 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 296 cacagccacc ggcacagac 19 297 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 297 guggccccuu gcagcgggc 19 298 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 298 ggcagcaguc agugggcag 19 299 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 299 cggcagcaca cugcucaug 19 300 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 300 gcuuggggcc cgugcagcc 19 301 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 301 aggccaggca gucagagug 19 302 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 302 ugugguugaa guggaggca 19 303 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 303 gcagcucaca gaugccacu 19 304 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 304 ugaccagggc ugggcagug 19 305 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 305 acgugucugu guuguaggu 19 306 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 306 gauugggcau ggacucaaa 19 307 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 307 auguauaccg gcccucggg 19 308 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 308 ucacacagcu ggcgccgaa 19 309 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 309 aguuguaggg acaggcagu 19 310 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 310 ccacguccgu agaaaggua 19 311 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 311 agacgagggu gcaggaucc 19 312 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 312 cuugguugug cagggggca 19 313 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 313 cauccucugc ugucaccuc 19 314 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 314 ucucacaccg cuguguucc 19 315 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 315 cacagggcuu gcugcacuu 19 316 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 316 gaccauagca cacucgggc 19 317 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 317 gcaagugcuc caugcccag 19 318 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 318 uaacugcccu caccucucg 19 319 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 319 ccuggauauu ggcacuggu 19 320 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 320 ucuugcagcc agcaaacuc 19 321 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 321 ccaggcuccc aaagaucuu 19 322 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 322 agcucuccgg cagaaaugc 19 323 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 323 aggcuggguc cccaucaaa 19 324 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 324 ggagcggggc aguguugga 19 325 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 325 cuuggagcug cucuggcug 19 326 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 326 cuuccagagu cucaaacac 19 327 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 327 auagguaacc ugugaucuc 19 328 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 328 ccggccaugc ugagaugua 19 329 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 329 ugaggucagg caggcuguc 19 330 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 330 gcagguucug gaagacgcu 19 331 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 331 uucguccccg gauuacuug 19 332 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 332 aggcgccauu gugcagaau 19 333 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 333 cuugcagggu cagcgagua 19 334 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 334 gccagcugau gcccagccc 19 335 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 335 ucagugagcg cagccccag 19 336 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 336 guccacugcc caguucccu 19 337 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 337 uaugguggau gagggccag 19 338 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 338 cgaagcagag guggguguu 19 339 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 339 cccagggcac cgugugcac 19 340 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 340 gguuccgaaa gagcugguc 19 341 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 341 ggagcagagc uuggugcgg 19 342 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 342 cuggccgguu ggcagugug 19 343 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 343 cgcccacaca cucguccuc 19 344 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 344 gguggcaggc caggcccuc 19 345 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 345 gcccucgggc gcacagcug 19 346 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 346 gcccuggacc ccagcagug 19 347 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 347 aguugacaca cuggguggg 19 348 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 348 cccgaaggaa cuggcugca 19 349 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 349 ccuccacgca cuccuggcc 19 350 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 350 ccugcaguac ucggcauuc 19 351 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 351 cauacucccu ggggagccc 19 352 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 352 aacagugccu ggcauucac 19 353 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 353 acucagggug gcacggcaa 19 354 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 354 agccauucug gggcugaca 19 355 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 355 guccaaaaca ggucacuga 19 356 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 356 cacacugguc agccuccgg 19 357 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 357 uauagugggc acaggccac 19 358 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 358 cgcagaaggg aggguccuu 19 359 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 359 cgcuggggca gcgggccac 19 360 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 360 agaggucagg uuucacacc 19 361 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 361 uccagauggg cauguagga 19 362 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 362 ccuccucauc uggaaacuu 19 363 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 363 ggcaaggcug gcaugcgcc 19 364 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 364 agugggugca guugauggg 19 365 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 365 cauccagguc cacacagga 19 366 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 366 cggcggggca gcccuuguc 19 367 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 367 gagggcuggc ucucugcuc 19 368 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 368 cagagaugau ggacgucag 19 369 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 369 gcagaaugcc aaccaccgc 19 370 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 370 cccccaagac cacgaccag 19 371 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 371 ugaggauccc aaagaccac 19 372 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 372 ucugcugccg ucgcuugau 19 373 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 373 ucguguacuu ccggaucuu 19 374 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 374 ccugcagcag ucuccgcau 19 375 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 375 gcuccaccag cuccguuuc 19 376 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 376 cuccgcuagg ugucagcgg 19 377 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 377 gcgccugguu gggcaucgc 19 378 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 378 cuuucaggau ccgcaucug 19 379 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 379 ccuuccucag cuccgucuc 19 380 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 380 cagauccaag caccuucac 19 381 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 381 agacugugcc aaaagcgcc 19 382 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 382 ggauccagau gcccuugua 19 383 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 383 ucacauucuc cccaucagg 19 384 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 384 ugauggccac uggaauuuu 19 385 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 385 uguuuucccu caacacuuu 19 386 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 386 uguuggcuuu gggggaugu 19 387 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 387 cuucgucuaa gauuucuuu 19 388 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 388 caccagccau cacguaugc 19 389 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 389 agacauaugg ggagcccac 19 390 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 390 agaugcccag aaggcggga 19 391 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 391 gcaccgugga ugucaggca 19 392 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 392 uaagcugugu caccagcug 19 393 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 393 agaggcagcc auagggcau 19 394 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 394 uuucccggac auggucuaa 19 395 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 395 agcccaggcg uccgcgguu 19 396 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 396 aguucagcag guccuggga 19 397 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 397 uggcaaucug cauacacca 19 398 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 398 ccagguagcu cauccccuu 19 399 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 399 guacgagccg cacauccuc 19 400 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 400 gagcggccaa gucccugug 19 401 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 401 ucuugaccag cacguuccg 19 402 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 402 uuuugacaug guugggacu 19 403 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 403 ccagcccgaa gucuguaau 19 404 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 404 caauguccag cagccgagc 19 405 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 405 caugguacuc ugucucguc 19 406 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 406 gcaccuugcc cccaucugc 19 407 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 407 gcgccaucca cuugauggg 19 408 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 408 ggcggagaau ggacuccag 19 409 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 409 ucuggugggu gaaccgccg 19 410 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 410 cauaacucca cacaucacu 19 411 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 411 gcucccacac agucacacc 19 412 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 412 uggccccaaa agucaucag 19 413 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 413 ggaucccauc guaagguuu 19 414 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 414 cagggaucuc ccgggcugg 19 415 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 415 cccccuuuuc cagcagguc 19 416 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 416 ggggcugggg cagccgcuc 19 417 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 417 caucaauggu gcagauggg 19 418 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 418 ugaccaugau cauguagac 19 419 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 419 agucaaucau ccaacauuu 19 420 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 420 aucuuggccg acauucaga 19 421 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 421 cagacaccaa cucccggaa 19 422 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 422 uggccaugcg ggagaauuc 19 423 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 423 caaagcgcug ggggucccu 19 424 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 424 ccucauucug gaugaccac 19 425 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 425 gacuggcugg gcccaaguc 19 426 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 426 agaaggugcu guccaaggg 19 427 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 427 ccuccagcag ugagcggua 19 428 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 428 ggucccccau gucaucguc 19 429 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 429 acuccucagc auccaccag 19 430 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 430 ccugcugggg uaccagaua 19 431 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 431 ggucuggaca gaagaagcc 19 432 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 432 ccccagcgcc cggggcagg 19 433 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 433 gccuguggug gaccaugcc 19 434 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 434 ugguagauga gcugcggug 19 435 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 435 gguccccacc gccacuccu 19 436 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 436 gcuccagccc uagugucag 19 437 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 437 gggccuccuc uucagaggg 19 438 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 438 gugccagugg agaccuggg 19 439 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 439 agccagcccc uucggaggg 19 440 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 440 caccaucaaa uacaucgga 19 441 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 441 cugcccccau ucccagguc 19 442 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 442 ggcuuugcag ccccuuggc 19 443 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 443 uggggucaug uguggggag 19 444 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 444 uguaccgcug uagagggcu 19 445 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 445 guacuguggg guccucacu 19 446 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 446 cagucucaga gggcagggg 19 447 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 447 ggggggcaac guagccauc 19 448 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 448 gcugggggcu gcaggucag 19 449 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 449 gcugguucac auauucagg 19 450 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 450 gcuggggccg aacaucugg 19 451 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 451 ccucucgggg cgaaggggg 19 452 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 452 gggcagcagg cagagggcc 19 453 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 453 gaguggcacc agcaggucg 19 454 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 454 gagucuuggg ccuuuccag 19 455 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 455 cauucuuccc uggggagag 19 456 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 456 aaacgucuuu gacgacccc 19 457 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 457 cggcaccccc aaaggcaaa 19 458 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 458 aguacucggg guucuccac 19 459 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 459 cuccucccug gggugucaa 19 460 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 460 gguggggcug aggggcagc 19 461 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 461 ggcugaaggc aggaggagg 19 462 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 462 agagguuguc gaaggcugg 19 463 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 463 gguccugguc ccaguaaua 19 464 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 464 gagccccccg cucuggugg 19 465 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 465 cuuugaaggu gcugggugg 19 466 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 466 ucucugccgu agguguccc 19 467 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 467 gacccaggua cucuggguu 19 468 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 468 uucacacugg cacguccag 19 469 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 469 gcggacuugg ccuucuggu 19 470 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 470 ggacacauca gggcuucug 19 471 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 471 gccuucccug cucccugag 19 472 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 472 gaugccagca gaagucagg 19 473 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 473 agggcccucc caccucuug 19 474 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 474 uccccuggaa guggucgga 19 475 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 475 guuccuggca uggcagguu 19 476 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 476 gaagguuccu uaggacagg 19 477 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 477 gggaacucaa gcaggaagg 19 478 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 478 gaccccuucc agccaucug 19 479 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 479 ccucuuccaa cgaggcugg 19 480 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 480 agacucccca gugcuguuc 19 481 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 481 gggccucaga auccacaaa 19 482 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 482 cuagagucuc auugggcag 19 483 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 483 uguggcaucc acuggaccc 19 484 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 484 gaaagggcca agcugggcu 19 485 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 485 guacccagga ucuggaagg 19 486 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 486 gcuucccuaa ggcuuucag 19 487 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 487 gcuuccccuc ucaggccag 19 488 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 488 agacacuccc uuagggccg 19 489 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 489 ugggucgcuu uuguucuua 19 490 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 490 ucagggacag ucucugaau 19 491 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 491 ggggggcagu acuagguuu 19 492 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 492 uugcuguucc uuccucaug 19 493 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 493 gccuggauac ugacaccau 19 494 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 494 aaaagcacuc uguacaaag 19 495 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 495 aaaguaaaaa cuaaacaga 19 496 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 496 uaaaaaaaca aaacaaaaa 19 497 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 497 gggucuuuau uucaucuuu 19 498 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 498 cucccccugg gucuuuauu 19 499 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 499 uccauggugc ucacugcggc u 21 500 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 500 agccgcagug agcaccaugg a 21 501 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 501 agguaccacg agugacgccg a 21 502 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 502 ucggcgucac ucgugguacc u 21 503 23 RNA Artificial Sequence Description of Artificial Sequence siNA antisense 503 uccauggugc ucacugcggc uuu 23 504 23 RNA Artificial Sequence Description of Artificial Sequence siNA sense 504 agccgcagug agcaccaugg auu 23 505 23 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 505 uccauggugc ucacugcggc uuu 23 506 23 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 506 agccgcagug agcaccaugg auu 23 507 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 507 uggggucguc aaagacguun n 21 508 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 508 aacgucuuug acgaccccan n 21 509 21 RNA Artificial Sequence misc_feature (20)..(21) n stands for thymidine 509 uugcagaaac ugcuggggun n 21 510 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 510 accccagcag uuucugcaan n 21 511 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 511 ggugcuugga ucuggcgcun n 21 512 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 512 agcgccagau ccaagcaccn n 21 513 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 513 ucgcggucua gguucguggn n 21 514 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 514 ccacgaaccu agaccgcgan n 21 515 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 515 gaucuuuggg agccuggcan n 21 516 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 516 ugccaggcuc ccaaagaucn n 21 517 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 517 acgguccgag gguuucuagn n 21 518 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 518 cuagaaaccc ucggaccgun n 21 519 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 519 ggugcuugga ucuggcgcun n 21 520 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 520 agcgccagau ccaagcaccn n 21 521 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 521 ggugcuugga ucuggcgcun n 21 522 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 522 ggugcuugga ucuggcgcun n 21 523 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 523 agcgccagau ccaagcaccn n 21 524 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 524 agcgccagau ccaagcaccn n 21 525 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 525 agcgccagau ccaagcaccn n 21 526 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 526 ucgcggucua gguucguggn n 21 527 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 527 ucgcggucua gguucguggn n 21 528 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense inverted control 528 ucgcggucua gguucguggn n 21 529 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 529 ccacgaaccu agaccgcgan n 21 530 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 530 ccacgaaccu agaccgcgan n 21 531 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 531 ccacgaaccu agaccgcgan n 21 532 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense inverted control 532 ccacgaaccu agaccgcgan n 21 533 23 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 533 uggucaccua caacacagac acg 23 534 23 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 534 cagaauggcu cagugaccug uuu 23 535 23 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 535 gcuuuguggu cauccagaau gag 23 536 23 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 536 agcaccuuca aagggacacc uac 23 537 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 537 gucaccuaca acacagacac g 21 538 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 538 gaauggcuca gugaccuguu u 21 539 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 539 uuugugguca uccagaauga g 21 540 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 540 caccuucaaa gggacaccua c 21 541 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 541 ugucuguguu guaggugacc a 21 542 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 542 acaggucacu gagccauucu g 21 543 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 543 cauucuggau gaccacaaag c 21 544 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 544 aggugucccu uugaaggugc u 21 545 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 545 gucaccuaca acacagacac g 21 546 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 546 gaauggcuca gugaccuguu u 21 547 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 547 uuugugguca uccagaauga g 21 548 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 548 caccuucaaa gggacaccua c 21 549 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 549 ugucuguguu guaggugacn n 21 550 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 550 acaggucacu gagccauucn n 21 551 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 551 cauucuggau gaccacaaan n 21 552 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 552 aggugucccu uugaaggugn n 21 553 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 553 cgcgcugcgc cggaguccc 19 554 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 554 cgagcuagcc ccggcgccg 19 555 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 555 gccgccgccc agaccggac 19 556 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 556 cgacaggcca ccucgucgg 19 557 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 557 gcguccgccc gaguccccg 19 558 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 558 gccucgccgc caacgccac 19 559 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 559 caaccaccgc gcacggccc 19 560 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 560 cccugacucc guccaguau 19 561 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 561 uugaucggga gagccggag 19 562 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 562 gcgagcucuu cggggagca 19 563 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 563 agcgaugcga cccuccggg 19 564 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 564 gacggccggg gcagcgcuc 19 565 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 565 ccuggcgcug cuggcugcg 19 566 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 566 gcucugcccg gcgagucgg 19 567 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 567 ggcucuggag gaaaagaaa 19 568 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 568 aguuugccaa ggcacgagu 19 569 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 569 uaacaagcuc acgcaguug 19 570 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 570 gggcacuuuu gaagaucau 19 571 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 571 uuuucucagc cuccagagg 19 572 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 572 gauguucaau aacugugag 19 573 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 573 ggugguccuu gggaauuug 19 574 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 574 ggaaauuacc uaugugcag 19 575 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 575 gaggaauuau gaucuuucc 19 576 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 576 cuucuuaaag accauccag 19 577 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 577 ggagguggcu gguuauguc 19 578 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 578 ccucauugcc cucaacaca 19 579 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 579 aguggagcga auuccuuug 19 580 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 580 ggaaaaccug cagaucauc 19 581 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 581 cagaggaaau auguacuac 19 582 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 582 cgaaaauucc uaugccuua 19 583 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 583 agcagucuua ucuaacuau 19 584 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 584 ugaugcaaau aaaaccgga 19 585 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 585 acugaaggag cugcccaug 19 586 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 586 gagaaauuua caggaaauc 19 587 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 587 ccugcauggc gccgugcgg 19 588 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 588 guucagcaac aacccugcc 19 589 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 589 ccugugcaac guggagagc 19 590 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 590 cauccagugg cgggacaua 19 591 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 591 agucagcagu gacuuucuc 19 592 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 592 cagcaacaug ucgauggac 19 593 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 593 cuuccagaac caccugggc 19 594 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 594 cagcugccaa aagugugau 19 595 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 595 uccaagcugu cccaauggg 19 596 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 596 gagcugcugg ggugcagga 19 597 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 597 agaggagaac ugccagaaa 19 598 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 598 acugaccaaa aucaucugu 19 599 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 599 ugcccagcag ugcuccggg 19 600 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 600 gcgcugccgu ggcaagucc 19 601 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 601 ccccagugac ugcugccac 19 602 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 602 caaccagugu gcugcaggc 19 603 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 603 cugcacaggc ccccgggag 19 604 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 604 gagcgacugc cuggucugc 19 605 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 605 ccgcaaauuc cgagacgaa 19 606 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 606 agccacgugc aaggacacc 19 607 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 607 cugcccccca cucaugcuc 19 608 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 608 cuacaacccc accacguac 19 609 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 609 ccagauggau gugaacccc 19 610 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 610 cgagggcaaa uacagcuuu 19 611 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 611 uggugccacc ugcgugaag 19 612 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 612 gaaguguccc cguaauuau 19 613 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 613 uguggugaca gaucacggc 19 614 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 614 cucgugcguc cgagccugu 19 615 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 615 uggggccgac agcuaugag 19 616 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 616 gauggaggaa gacggcguc 19 617 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 617 ccgcaagugu aagaagugc 19 618 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 618 cgaagggccu ugccgcaaa 19 619 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 619 aguguguaac ggaauaggu 19 620 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 620 uauuggugaa uuuaaagac 19 621 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 621 cucacucucc auaaaugcu 19 622 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 622 uacgaauauu aaacacuuc 19 623 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 623 caaaaacugc accuccauc 19 624 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 624 caguggcgau cuccacauc 19 625 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 625 ccugccggug gcauuuagg 19 626 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 626 gggugacucc uucacacau 19 627 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 627 uacuccuccu cuggaucca 19 628 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 628 acaggaacug gauauucug 19 629 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 629 gaaaaccgua aaggaaauc 19 630 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 630 cacaggguuu uugcugauu 19 631 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 631 ucaggcuugg ccugaaaac 19 632 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 632 caggacggac cuccaugcc 19 633 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 633 cuuugagaac cuagaaauc 19 634 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 634 cauacgcggc aggaccaag 19 635 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 635 gcaacauggu caguuuucu 19 636 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 636 ucuugcaguc gucagccug 19 637 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 637 gaacauaaca uccuuggga 19 638 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 638 auuacgcucc cucaaggag 19 639 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 639 gauaagugau ggagaugug 19 640 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 640 gauaauuuca ggaaacaaa 19 641 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 641 aaauuugugc uaugcaaau 19 642 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 642 uacaauaaac uggaaaaaa 19 643 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 643 acuguuuggg accuccggu 19 644 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 644 ucagaaaacc aaaauuaua 19 645 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 645 aagcaacaga ggugaaaac 19 646 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 646 cagcugcaag gccacaggc 19 647 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 647 ccaggucugc caugccuug 19 648 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 648 gugcuccccc gagggcugc 19 649 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 649 cuggggcccg gagcccagg 19 650 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 650 ggacugcguc ucuugccgg 19 651 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 651 gaaugucagc cgaggcagg 19 652 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 652 ggaaugcgug gacaagugc 19 653 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 653 caagcuucug gagggugag 19 654 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 654 gccaagggag uuuguggag 19 655 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 655 gaacucugag ugcauacag 19 656 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 656 gugccaccca gagugccug 19 657 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 657 gccucaggcc augaacauc 19 658 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 658 caccugcaca ggacgggga 19 659 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 659 accagacaac uguauccag 19 660 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 660 gugugcccac uacauugac 19 661 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 661 cggcccccac ugcgucaag 19 662 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 662 gaccugcccg gcaggaguc 19 663 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 663 caugggagaa aacaacacc 19 664 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 664 ccuggucugg aaguacgca 19 665 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 665 agacgccggc caugugugc 19 666 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 666 ccaccugugc cauccaaac 19 667 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 667 cugcaccuac ggaugcacu 19 668 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 668 ugggccaggu cuugaaggc 19 669 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 669 cuguccaacg aaugggccu 19 670 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 670 uaagaucccg uccaucgcc 19 671 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 671 cacugggaug gugggggcc 19 672 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 672 ccuccucuug cugcuggug 19 673 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 673 gguggcccug gggaucggc 19 674 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 674 ccucuucaug cgaaggcgc 19 675 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 675 ccacaucguu cggaagcgc 19 676 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 676 cacgcugcgg aggcugcug 19 677 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 677 gcaggagagg gagcuugug 19 678 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 678 ggagccucuu acacccagu 19 679 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 679 uggagaagcu cccaaccaa 19 680 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 680 agcucucuug aggaucuug 19 681 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 681 gaaggaaacu gaauucaaa 19 682 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 682 aaagaucaaa gugcugggc 19 683 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 683 cuccggugcg uucggcacg 19 684 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 684 gguguauaag ggacucugg 19 685 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 685 gaucccagaa ggugagaaa 19 686 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 686 aguuaaaauu cccgucgcu 19 687 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 687 uaucaaggaa uuaagagaa 19 688 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 688 agcaacaucu ccgaaagcc 19 689 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 689 caacaaggaa auccucgau 19 690 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 690 ugaagccuac gugauggcc 19 691 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 691 cagcguggac aacccccac 19 692 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 692 cgugugccgc cugcugggc 19 693 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 693 caucugccuc accuccacc 19 694 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 694 cgugcaacuc aucacgcag 19 695 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 695 gcucaugccc uucggcugc 19 696 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 696 ccuccuggac uauguccgg 19 697 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 697 ggaacacaaa gacaauauu 19 698 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 698 uggcucccag uaccugcuc 19 699 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 699 caacuggugu gugcagauc 19 700 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 700 cgcaaagggc augaacuac 19 701 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 701 cuuggaggac cgucgcuug 19 702 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 702 ggugcaccgc gaccuggca 19 703 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 703 agccaggaac guacuggug 19 704 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 704 gaaaacaccg cagcauguc 19 705 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 705 caagaucaca gauuuuggg 19 706 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 706 gcuggccaaa cugcugggu 19 707 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 707 ugcggaagag aaagaauac 19 708 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 708 ccaugcagaa ggaggcaaa 19 709 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 709 agugccuauc aaguggaug 19 710 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 710 ggcauuggaa ucaauuuua 19 711 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 711 acacagaauc uauacccac 19 712 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 712 ccagagugau gucuggagc 19 713 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 713 cuacggggug accguuugg 19 714 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 714 ggaguugaug accuuugga 19 715 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 715 auccaagcca uaugacgga 19 716 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 716 aaucccugcc agcgagauc 19 717 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 717 cuccuccauc cuggagaaa 19 718 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 718 aggagaacgc cucccucag 19 719 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 719 gccacccaua uguaccauc 19 720 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 720 cgaugucuac augaucaug 19 721 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 721 ggucaagugc uggaugaua 19 722 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 722 agacgcagau agucgccca 19 723 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 723 aaaguuccgu gaguugauc 19 724 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 724 caucgaauuc uccaaaaug 19 725 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 725 ggcccgagac ccccagcgc 19 726 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 726 cuaccuuguc auucagggg 19 727 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 727 ggaugaaaga augcauuug 19 728 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 728 gccaaguccu acagacucc 19 729 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 729 caacuucuac cgugcccug 19 730 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 730 gauggaugaa gaagacaug 19 731 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 731 ggacgacgug guggaugcc 19 732 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 732 cgacgaguac cucauccca 19 733 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 733 acagcagggc uucuucagc 19 734 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 734 cagccccucc acgucacgg 19 735 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 735 gacuccccuc cugagcucu 19 736 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 736 ucugagugca accagcaac 19 737 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 737 caauuccacc guggcuugc 19 738 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 738 cauugauaga aaugggcug 19 739 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 739 gcaaagcugu cccaucaag 19 740 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 740 ggaagacagc uucuugcag 19 741 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 741 gcgauacagc ucagacccc 19 742 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 742 cacaggcgcc uugacugag 19 743 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 743 ggacagcaua gacgacacc 19 744 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 744 cuuccuccca gugccugaa 19 745 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 745 auacauaaac caguccguu 19 746 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 746 ucccaaaagg cccgcuggc 19 747 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 747 cucugugcag aauccuguc 19 748 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 748 cuaucacaau cagccucug 19 749 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 749 gaaccccgcg cccagcaga 19 750 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 750 agacccacac uaccaggac 19 751 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 751 cccccacagc acugcagug 19 752 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 752 gggcaacccc gaguaucuc 19 753 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 753 caacacuguc cagcccacc 19 754 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 754 cugugucaac agcacauuc 19 755 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 755 cgacagcccu gcccacugg 19 756 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 756 ggcccagaaa ggcagccac 19 757 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 757 ccaaauuagc cuggacaac 19 758 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 758 cccugacuac cagcaggac 19 759 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 759 cuucuuuccc aaggaagcc 19 760 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 760 caagccaaau ggcaucuuu 19 761 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 761 uaagggcucc acagcugaa 19 762 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 762 aaaugcagaa uaccuaagg 19 763 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 763 ggucgcgcca caaagcagu 19 764 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 764 ugaauuuauu ggagcauga 19 765 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 765 accacggagg auaguauga 19 766 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 766 agcccuaaaa auccagacu 19 767 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 767 ucuuucgaua cccaggacc 19 768 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 768 caagccacag cagguccuc 19 769 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 769 ccaucccaac agccaugcc 19 770 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 770 ccgcauuagc ucuuagacc 19 771 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 771 ccacagacug guuuugcaa 19 772 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 772 acguuuacac cgacuagcc 19 773 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 773 caggaaguac uuccaccuc 19 774 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 774 cgggcacauu uugggaagu 19 775 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 775 uugcauuccu uugucuuca 19 776 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 776 aaacugugaa gcauuuaca 19 777 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 777 agaaacgcau ccagcaaga 19 778 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 778 aauauugucc cuuugagca 19 779 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 779 agaaauuuau cuuucaaag 19 780 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 780 gagguauauu ugaaaaaaa 19 781 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 781 aaaaaaaaag uauauguga 19 782 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 782 aggauuuuua uugauuggg 19 783 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 783 ggaucuugga guuuuucau 19 784 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 784 uugucgcuau ugauuuuua 19 785 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 785 acuucaaugg gcucuucca 19 786 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 786 aacaaggaag aagcuugcu 19 787 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 787 ugguagcacu ugcuacccu 19 788 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 788 ugaguucauc caggcccaa 19 789 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 789 acugugagca aggagcaca 19 790 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 790 aagccacaag ucuuccaga 19 791 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 791 aggaugcuug auuccagug 19 792 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 792 gguucugcuu caaggcuuc 19 793 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 793 ccacugcaaa acacuaaag 19 794 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 794 gauccaagaa ggccuucau 19 795 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 795 uggccccagc aggccggau 19 796 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 796 ucgguacugu aucaaguca 19 797 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 797 auggcaggua caguaggau 19 798 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 798 uaagccacuc ugucccuuc 19 799 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 799 ccugggcaaa gaagaaacg 19 800 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 800 ggaggggaug aauucuucc 19 801 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 801 cuuagacuua cuuuuguaa 19 802 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 802 aaaauguccc cacgguacu 19 803 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 803 uuacucccca cugauggac 19 804 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 804 ccagugguuu ccagucaug 19 805 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 805 gagcguuaga cugacuugu 19 806 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 806 uuugucuucc auuccauug 19 807 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 807 guuuugaaac ucaguaugc 19 808 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 808 ccgccccugu cuugcuguc 19 809 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 809 caugaaauca gcaagagag 19 810 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 810 ggaugacaca ucaaauaau 19 811 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 811 uaacucggau uccagccca 19 812 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 812 acauuggauu caucagcau 19 813 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 813 uuuggaccaa uagcccaca 19 814 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 814 agcugagaau guggaauac 19 815 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 815 ccuaaggaua acaccgcuu 19 816 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 816 uuuguucucg caaaaacgu 19 817 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 817 uaucuccuaa uuugaggcu 19 818 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 818 ucagaugaaa ugcaucagg 19 819 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 819 guccuuuggg gcauagauc 19 820 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 820 cagaagacua caaaaauga 19 821 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 821 aagcugcucu gaaaucucc 19 822 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 822 cuuuagccau caccccaac 19 823 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 823 ccccccaaaa uuaguuugu 19 824 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 824 uguuacuuau ggaagauag 19 825 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 825 guuuucuccu uuuacuuca 19 826 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 826 acuucaaaag cuuuuuacu 19 827 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 827 ucaaagagua uauguuccc 19 828 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 828 cuccagguca gcugccccc 19 829 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 829 caaacccccu ccuuacgcu 19 830 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 830 uuugucacac aaaaagugu 19 831 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 831 ucucugccuu gagucaucu 19 832 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 832 uauucaagca cuuacagcu 19 833 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 833 ucuggccaca acagggcau 19 834 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 834 uuuuacaggu gcgaaugac 19 835 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 835 caguagcauu augaguagu 19 836 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 836 ugugaauuca gguaguaaa 19 837 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 837 auaugaaacu aggguuuga 19 838 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 838 aaauugauaa ugcuuucac 19 839 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 839 caacauuugc agauguuuu 19 840 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 840 uagaaggaaa aaaguuccu 19 841 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 841 uuccuaaaau aauuucucu 19 842 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 842 uacaauugga agauuggaa 19 843 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 843 agauucagcu aguuaggag 19 844 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 844 gcccauuuuu uccuaaucu 19 845 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 845 ugugugugcc cuguaaccu 19 846 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 846 ugacugguua acagcaguc 19 847 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 847 ccuuuguaaa caguguuuu 19 848 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 848 uaaacucucc uagucaaua 19 849 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 849 auccacccca uccaauuua 19 850 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 850 aucaaggaag aaaugguuc 19 851 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 851 cagaaaauau uuucagccu 19 852 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 852 uacaguuaug uucagucac 19 853 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 853 cacacacaua caaaauguu 19 854 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 854 uccuuuugcu uuuaaagua 19 855 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 855 aauuuuugac ucccagauc 19 856 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 856 cagucagagc cccuacagc 19 857 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 857 cauuguuaag aaaguauuu 19 858 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 858 ugauuuuugu cucaaugaa 19 859 19 RNA Artificial Sequence Description of Artificial Sequence Target sequence/siNA sense region 859 aaauaaaacu auauucauu 19 860 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 860 gggacuccgg cgcagcgcg 19 861 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 861 cggcgccggg gcuagcucg 19 862 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 862 guccggucug ggcggcggc 19 863 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 863 ccgacgaggu ggccugucg 19 864 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 864 cggggacucg ggcggacgc 19 865 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 865 guggcguugg cggcgaggc 19 866 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 866 gggccgugcg cggugguug 19 867 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 867 auacuggacg gagucaggg 19 868 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 868 cuccggcucu cccgaucaa 19 869 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 869 ugcuccccga agagcucgc 19 870 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 870 cccggagggu cgcaucgcu 19 871 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 871 gagcgcugcc ccggccguc 19 872 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 872 cgcagccagc agcgccagg 19 873 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 873 ccgacucgcc gggcagagc 19 874 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 874 uuucuuuucc uccagagcc 19 875 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 875 acucgugccu uggcaaacu 19 876 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 876 caacugcgug agcuuguua 19 877 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 877 augaucuuca aaagugccc 19 878 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 878 ccucuggagg cugagaaaa 19 879 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 879 cucacaguua uugaacauc 19 880 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 880 caaauuccca aggaccacc 19 881 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 881 cugcacauag guaauuucc 19 882 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 882 ggaaagauca uaauuccuc 19 883 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 883 cuggaugguc uuuaagaag 19 884 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 884 gacauaacca gccaccucc 19 885 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 885 uguguugagg gcaaugagg 19 886 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 886 caaaggaauu cgcuccacu 19 887 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 887 gaugaucugc agguuuucc 19 888 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 888 guaguacaua uuuccucug 19 889 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 889 uaaggcauag gaauuuucg 19 890 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 890 auaguuagau aagacugcu 19 891 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 891 uccgguuuua uuugcauca 19 892 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 892 caugggcagc uccuucagu 19 893 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 893 gauuuccugu aaauuucuc 19 894 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 894 ccgcacggcg ccaugcagg 19 895 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 895 ggcaggguug uugcugaac 19 896 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 896 gcucuccacg uugcacagg 19 897 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 897 uaugucccgc cacuggaug 19 898 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 898 gagaaaguca cugcugacu 19 899 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 899 guccaucgac auguugcug 19 900 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 900 gcccaggugg uucuggaag 19 901 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 901 aucacacuuu uggcagcug 19 902 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 902 cccauuggga cagcuugga 19 903 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 903 uccugcaccc cagcagcuc 19 904 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 904 uuucuggcag uucuccucu 19 905 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 905 acagaugauu uuggucagu 19 906 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 906 cccggagcac ugcugggca 19 907 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 907 ggacuugcca cggcagcgc 19 908 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 908 guggcagcag ucacugggg 19 909 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 909 gccugcagca cacugguug 19 910 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 910 cucccggggg ccugugcag 19 911 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 911 gcagaccagg cagucgcuc 19 912 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 912 uucgucucgg aauuugcgg 19 913 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 913 gguguccuug cacguggcu 19 914 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 914 gagcaugagu ggggggcag 19 915 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 915 guacguggug ggguuguag 19 916 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 916 gggguucaca uccaucugg 19 917 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 917 aaagcuguau uugcccucg 19 918 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 918 cuucacgcag guggcacca 19 919 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 919 auaauuacgg ggacacuuc 19 920 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 920 gccgugaucu gucaccaca 19 921 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 921 acaggcucgg acgcacgag 19 922 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 922 cucauagcug ucggcccca 19 923 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 923 gacgccgucu uccuccauc 19 924 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 924 gcacuucuua cacuugcgg 19 925 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 925 uuugcggcaa ggcccuucg 19 926 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 926 accuauuccg uuacacacu 19 927 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 927 gucuuuaaau ucaccaaua 19 928 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 928 agcauuuaug gagagugag 19 929 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 929 gaaguguuua auauucgua 19 930 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 930 gauggaggug caguuuuug 19 931 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 931 gauguggaga ucgccacug 19 932 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 932 ccuaaaugcc accggcagg 19 933 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 933 augugugaag gagucaccc 19 934 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 934 uggauccaga ggaggagua 19 935 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 935 cagaauaucc aguuccugu 19 936 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 936 gauuuccuuu acgguuuuc 19 937 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 937 aaucagcaaa aacccugug 19 938 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 938 guuuucaggc caagccuga 19 939 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 939 ggcauggagg uccguccug 19 940 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 940 gauuucuagg uucucaaag 19 941 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 941 cuugguccug ccgcguaug 19 942 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 942 agaaaacuga ccauguugc 19 943 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 943 caggcugacg acugcaaga 19 944 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 944 ucccaaggau guuauguuc 19 945 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 945 cuccuugagg gagcguaau 19 946 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 946 cacaucucca ucacuuauc 19 947 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 947 uuuguuuccu gaaauuauc 19 948 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 948 auuugcauag cacaaauuu 19 949 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 949 uuuuuuccag uuuauugua 19 950 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 950 accggagguc ccaaacagu 19 951 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 951 uauaauuuug guuuucuga 19 952 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 952 guuuucaccu cuguugcuu 19 953 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 953 gccuguggcc uugcagcug 19 954 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 954 caaggcaugg cagaccugg 19 955 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 955 gcagcccucg ggggagcac 19 956 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 956 ccugggcucc gggccccag 19 957 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 957 ccggcaagag acgcagucc 19 958 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 958 ccugccucgg cugacauuc 19 959 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 959 gcacuugucc acgcauucc 19 960 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 960 cucacccucc agaagcuug 19 961 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 961 cuccacaaac ucccuuggc 19 962 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 962 cuguaugcac ucagaguuc 19 963 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 963 caggcacucu ggguggcac 19 964 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 964 gauguucaug gccugaggc 19 965 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 965 uccccguccu gugcaggug 19 966 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 966 cuggauacag uugucuggu 19 967 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 967 gucaauguag ugggcacac 19 968 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 968 cuugacgcag ugggggccg 19 969 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 969 gacuccugcc gggcagguc 19 970 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 970 gguguuguuu ucucccaug 19 971 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 971 ugcguacuuc cagaccagg 19 972 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 972 gcacacaugg ccggcgucu 19 973 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 973 guuuggaugg cacaggugg 19 974 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 974 agugcauccg uaggugcag 19 975 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 975 gccuucaaga ccuggccca 19 976 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 976 aggcccauuc guuggacag 19 977 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 977 ggcgauggac gggaucuua 19 978 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 978 ggcccccacc aucccagug 19 979 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 979 caccagcagc aagaggagg 19 980 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 980 gccgaucccc agggccacc 19 981 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 981 gcgccuucgc augaagagg 19 982 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 982 gcgcuuccga acgaugugg 19 983 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 983 cagcagccuc cgcagcgug 19 984 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 984 cacaagcucc cucuccugc 19 985 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 985 acugggugua agaggcucc 19 986 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 986 uugguuggga gcuucucca 19 987 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 987 caagauccuc aagagagcu 19 988 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 988 uuugaauuca guuuccuuc 19 989 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 989 gcccagcacu uugaucuuu 19 990 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 990 cgugccgaac gcaccggag 19 991 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 991 ccagaguccc uuauacacc 19 992 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 992 uuucucaccu ucugggauc 19 993 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 993 agcgacggga auuuuaacu 19 994 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 994 uucucuuaau uccuugaua 19 995 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 995 ggcuuucgga gauguugcu 19 996 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 996 aucgaggauu uccuuguug 19 997 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 997 ggccaucacg uaggcuuca 19 998 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 998 guggggguug uccacgcug 19 999 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 999 gcccagcagg cggcacacg 19 1000 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1000 gguggaggug aggcagaug 19 1001 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1001 cugcgugaug aguugcacg 19 1002 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1002 gcagccgaag ggcaugagc 19 1003 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1003 ccggacauag uccaggagg 19 1004 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1004 aauauugucu uuguguucc 19 1005 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1005 gagcagguac ugggagcca 19 1006 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1006 gaucugcaca caccaguug 19 1007 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1007 guaguucaug cccuuugcg 19 1008 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1008 caagcgacgg uccuccaag 19 1009 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1009 ugccaggucg cggugcacc 19 1010 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1010 caccaguacg uuccuggcu 19 1011 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1011 gacaugcugc gguguuuuc 19 1012 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1012 cccaaaaucu gugaucuug 19 1013 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1013 acccagcagu uuggccagc 19 1014 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1014 guauucuuuc ucuuccgca 19 1015 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1015 uuugccuccu ucugcaugg 19 1016 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1016 cauccacuug auaggcacu 19 1017 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1017 uaaaauugau uccaaugcc 19 1018 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1018 guggguauag auucugugu 19 1019 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1019 gcuccagaca ucacucugg 19 1020 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1020 ccaaacgguc accccguag 19 1021 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1021 uccaaagguc aucaacucc 19 1022 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1022 uccgucauau ggcuuggau 19 1023 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1023 gaucucgcug gcagggauu 19 1024 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1024 uuucuccagg auggaggag 19 1025 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1025 cugagggagg cguucuccu 19 1026 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1026 gaugguacau auggguggc 19 1027 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1027 caugaucaug uagacaucg 19 1028 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1028 uaucauccag cacuugacc 19 1029 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1029 ugggcgacua ucugcgucu 19 1030 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1030 gaucaacuca cggaacuuu 19 1031 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1031 cauuuuggag aauucgaug 19 1032 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1032 gcgcuggggg ucucgggcc 19 1033 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1033 ccccugaaug acaagguag 19 1034 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1034 caaaugcauu cuuucaucc 19 1035 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1035 ggagucugua ggacuuggc 19 1036 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1036 cagggcacgg uagaaguug 19 1037 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1037 caugucuucu ucauccauc 19 1038 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1038 ggcauccacc acgucgucc 19 1039 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1039 ugggaugagg uacucgucg 19 1040 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1040 gcugaagaag cccugcugu 19 1041 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1041 ccgugacgug gaggggcug 19 1042 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1042 agagcucagg aggggaguc 19 1043 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1043 guugcugguu gcacucaga 19 1044 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1044 gcaagccacg guggaauug 19 1045 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1045 cagcccauuu cuaucaaug 19 1046 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1046 cuugauggga cagcuuugc 19 1047 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1047 cugcaagaag cugucuucc 19 1048 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1048 ggggucugag cuguaucgc 19 1049 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1049 cucagucaag gcgccugug 19 1050 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1050 ggugucgucu augcugucc 19 1051 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1051 uucaggcacu gggaggaag 19 1052 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1052 aacggacugg uuuauguau 19 1053 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1053 gccagcgggc cuuuuggga 19 1054 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1054 gacaggauuc ugcacagag 19 1055 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1055 cagaggcuga uugugauag 19 1056 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1056 ucugcugggc gcgggguuc 19 1057 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1057 guccugguag ugugggucu 19 1058 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1058 cacugcagug cuguggggg 19 1059 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1059 gagauacucg ggguugccc 19 1060 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1060 ggugggcugg acaguguug 19 1061 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1061 gaaugugcug uugacacag 19 1062 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1062 ccagugggca gggcugucg 19 1063 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1063 guggcugccu uucugggcc 19 1064 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1064 guuguccagg cuaauuugg 19 1065 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1065 guccugcugg uagucaggg 19 1066 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1066 ggcuuccuug ggaaagaag 19 1067 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1067 aaagaugcca uuuggcuug 19 1068 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1068 uucagcugug gagcccuua 19 1069 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1069 ccuuagguau ucugcauuu 19 1070 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1070 acugcuuugu ggcgcgacc 19 1071 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1071 ucaugcucca auaaauuca 19 1072 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1072 ucauacuauc cuccguggu 19 1073 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1073 agucuggauu uuuagggcu 19 1074 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1074 gguccugggu aucgaaaga 19 1075 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1075 gaggaccugc uguggcuug 19 1076 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1076 ggcauggcug uugggaugg 19 1077 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1077 ggucuaagag cuaaugcgg 19 1078 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1078 uugcaaaacc agucugugg 19 1079 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1079 ggcuagucgg uguaaacgu 19 1080 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1080 gagguggaag uacuuccug 19 1081 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1081 acuucccaaa augugcccg 19 1082 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1082 ugaagacaaa ggaaugcaa 19 1083 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1083 uguaaaugcu ucacaguuu 19 1084 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1084 ucuugcugga ugcguuucu 19 1085 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1085 ugcucaaagg gacaauauu 19 1086 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1086 cuuugaaaga uaaauuucu 19 1087 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1087 uuuuuuucaa auauaccuc 19 1088 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1088 ucacauauac uuuuuuuuu 19 1089 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1089 cccaaucaau aaaaauccu 19 1090 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1090 augaaaaacu ccaagaucc 19 1091 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1091 uaaaaaucaa uagcgacaa 19 1092 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1092 uggaagagcc cauugaagu 19 1093 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1093 agcaagcuuc uuccuuguu 19 1094 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1094 aggguagcaa gugcuacca 19 1095 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1095 uugggccugg augaacuca 19 1096 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1096 ugugcuccuu gcucacagu 19 1097 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1097 ucuggaagac uuguggcuu 19 1098 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1098 cacuggaauc aagcauccu 19 1099 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1099 gaagccuuga agcagaacc 19 1100 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1100 cuuuaguguu uugcagugg 19 1101 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1101 augaaggccu ucuuggauc 19 1102 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1102 auccggccug cuggggcca 19 1103 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1103 ugacuugaua caguaccga 19 1104 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1104 auccuacugu accugccau 19 1105 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1105 gaagggacag aguggcuua 19 1106 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1106 cguuucuucu uugcccagg 19 1107 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1107 ggaagaauuc auccccucc 19 1108 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1108 uuacaaaagu aagucuaag 19 1109 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1109 aguaccgugg ggacauuuu 19 1110 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1110 guccaucagu ggggaguaa 19 1111 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1111 caugacugga aaccacugg 19 1112 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1112 acaagucagu cuaacgcuc 19 1113 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1113 caauggaaug gaagacaaa 19 1114 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1114 gcauacugag uuucaaaac 19 1115 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1115 gacagcaaga caggggcgg 19 1116 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1116 cucucuugcu gauuucaug 19 1117 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1117 auuauuugau gugucaucc 19 1118 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1118 ugggcuggaa uccgaguua 19 1119 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1119 augcugauga auccaaugu 19 1120 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1120 ugugggcuau ugguccaaa 19 1121 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1121 guauuccaca uucucagcu 19 1122 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1122 aagcgguguu auccuuagg 19 1123 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1123 acguuuuugc gagaacaaa 19 1124 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1124 agccucaaau uaggagaua 19 1125 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1125 ccugaugcau uucaucuga 19 1126 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1126 gaucuaugcc ccaaaggac 19 1127 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1127 ucauuuuugu agucuucug 19 1128 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1128 ggagauuuca gagcagcuu 19 1129 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1129 guugggguga uggcuaaag 19 1130 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1130 acaaacuaau uuugggggg 19 1131 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1131 cuaucuucca uaaguaaca 19 1132 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1132 ugaaguaaaa ggagaaaac 19 1133 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1133 aguaaaaagc uuuugaagu 19 1134 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1134 gggaacauau acucuuuga 19 1135 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1135 gggggcagcu gaccuggag 19 1136 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1136 agcguaagga ggggguuug 19 1137 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1137 acacuuuuug ugugacaaa 19 1138 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1138 agaugacuca aggcagaga 19 1139 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1139 agcuguaagu gcuugaaua 19 1140 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1140 augcccuguu guggccaga 19 1141 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1141 gucauucgca ccuguaaaa 19 1142 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1142 acuacucaua augcuacug 19 1143 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1143 uuuacuaccu gaauucaca 19 1144 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1144 ucaaacccua guuucauau 19 1145 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1145 gugaaagcau uaucaauuu 19 1146 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1146 aaaacaucug caaauguug 19 1147 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1147 aggaacuuuu uuccuucua 19 1148 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1148 agagaaauua uuuuaggaa 19 1149 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1149 uuccaaucuu ccaauugua 19 1150 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1150 cuccuaacua gcugaaucu 19 1151 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1151 agauuaggaa aaaaugggc 19 1152 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1152 agguuacagg gcacacaca 19 1153 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1153 gacugcuguu aaccaguca 19 1154 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1154 aaaacacugu uuacaaagg 19 1155 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1155 uauugacuag gagaguuua 19 1156 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1156 uaaauuggau gggguggau 19 1157 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1157 gaaccauuuc uuccuugau 19 1158 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1158 aggcugaaaa uauuuucug 19 1159 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1159 gugacugaac auaacugua 19 1160 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1160 aacauuuugu augugugug 19 1161 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1161 uacuuuaaaa gcaaaagga 19 1162 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1162 gaucugggag ucaaaaauu 19 1163 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1163 gcuguagggg cucugacug 19 1164 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1164 aaauacuuuc uuaacaaug 19 1165 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1165 uucauugaga caaaaauca 19 1166 19 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1166 aaugaauaua guuuuauuu 19 1167 23 RNA Artificial Sequence Description of Artificial Sequence Targe t sequence/siNA sense region 1167 gagaacugcc agaaacugac caa 23 1168 23 RNA Artificial Sequence Description of Artificial Sequence Targe t sequence/siNA sense region 1168 aaaggaaauc acaggguuuu ugc 23 1169 23 RNA Artificial Sequence Description of Artificial Sequence Targe t sequence/siNA sense region 1169 aaguuccgug aguugaucau cga 23 1170 23 RNA Artificial Sequence Description of Artificial Sequence Targe t sequence/siNA sense region 1170 ugccaagucc uacagacucc aac 23 1171 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1171 gaacugccag aaacugacca a 21 1172 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1172 aggaaaucac aggguuuuug c 21 1173 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1173 guuccgugag uugaucaucg a 21 1174 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1174 ccaaguccua cagacuccaa c 21 1175 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1175 ggucaguuuc uggcaguucu c 21 1176 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1176 aaaaacccug ugauuuccuu u 21 1177 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1177 gaugaucaac ucacggaacu u 21 1178 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1178 uggagucugu aggacuuggc a 21 1179 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1179 gaacugccag aaacugacca a 21 1180 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1180 aggaaaucac aggguuuuug c 21 1181 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1181 guuccgugag uugaucaucg a 21 1182 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1182 ccaaguccua cagacuccaa c 21 1183 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1183 ggucaguuuc uggcaguucn n 21 1184 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1184 aaaaacccug ugauuuccun n 21 1185 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1185 gaugaucaac ucacggaacn n 21 1186 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1186 uggagucugu aggacuuggn n 21 1187 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1187 nnnnnnnnnn nnnnnnnnnn n 21 1188 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1188 nnnnnnnnnn nnnnnnnnnn n 21 1189 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1189 nnnnnnnnnn nnnnnnnnnn n 21 1190 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1190 nnnnnnnnnn nnnnnnnnnn n 21 1191 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1191 nnnnnnnnnn nnnnnnnnnn n 21 1192 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1192 nnnnnnnnnn nnnnnnnnnn n 21 1193 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1193 nnnnnnnnnn nnnnnnnnnn n 21 1194 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1194 nnnnnnnnnn nnnnnnnnnn n 21 1195 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1195 nnnnnnnnnn nnnnnnnnnn n 21 1196 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1196 augcuccgcc accucuaccn n 21 1197 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1197 gguagaggug gcggagcaun n 21 1198 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1198 augcuccgcc accucuaccn n 21 1199 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1199 gguagaggug gcggagcaun n 21 1200 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1200 augcuccgcc accucuaccn n 21 1201 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1201 augcuccgcc accucuaccn n 21 1202 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1202 gguagaggug gcggagcaun n 21 1203 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1203 gguagaggug gcggagcaun n 21 1204 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1204 gguagaggug gcggagcaun n 21 1205 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1205 uuugucuucc auuccauugn n 21 1206 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1206 caauggaaug gaagacaaan n 21 1207 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1207 uuugucuucc auuccauugn n 21 1208 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1208 caauggaaug gaagacaaan n 21 1209 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1209 uuugucuucc auuccauugn n 21 1210 21 RNA Artificial Sequence Description of Artificial Sequence siNA sense region 1210 uuugucuucc auuccauugn n 21 1211 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1211 caauggaaug gaagacaaan n 21 1212 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1212 caauggaaug gaagacaaan n 21 1213 21 RNA Artificial Sequence Description of Artificial Sequence siNA antisense region 1213 caauggaaug gaagacaaan n 21 

What we claim is:
 1. A short interfering nucleic acid (siNA) molecule that down regulates expression of an EGFR gene by RNA interference.
 2. The siNA molecule of claim 1, wherein the EGFR gene comprises HER1 sequence, HER2 sequence, HER3 sequence, HER4 sequence, or a combination thereof.
 3. The siNA molecule of claim 1, wherein said siNA molecule is adapted for use to treat cancer.
 4. The siNA molecule of claim 1, wherein said siNA molecule comprises a sense region and an antisense region and wherein said antisense region comprises sequence complementary to an RNA sequence encoding EGFR and the sense region comprises sequence complementary to the antisense region.
 5. The siNA molecule of claim 4, wherein said siNA molecule is assembled from two nucleic acid fragments wherein one fragment comprises the sense region and the second fragment comprises the antisense region of said siNA molecule.
 6. The siNA molecule of claim 5, wherein said sense region and antisense region are covalently connected via a linker molecule.
 7. The siNA molecule of claim 6, wherein said linker molecule is a polynucleotide linker.
 8. The siNA molecule of claim 6, wherein said linker molecule is a non-nucleotide linker.
 9. The siNA molecule of claim 1, wherein the siNA molecule comprises sequence having any of SEQ ID NOs. 1-1213.
 10. The siNA molecule of claim 4, wherein said sense region comprises a 3′-terminal overhang and said antisense region comprises a 3′-terminal overhang.
 11. The siNA molecule of claim 10, wherein said 3′-terminal overhangs each comprise about 2 nucleotides.
 12. The siNA molecule of claim 10, wherein said antisense region 3′-terminal nucleotide overhang is complementary to RNA encoding EGFR.
 13. The siNA molecule of claim 4, wherein said sense region comprises one or more 2′-O-methyl modified pyrimidine nucleotides.
 14. The siNA molecule of claim 4, wherein said sense region comprises a terminal cap moiety at the 5′-end, 3′-end, or both 5′ and 3′ ends of said sense region.
 15. The siNA molecule of claim 4, wherein said antisense region comprises one or more 2′-deoxy-2′-fluoro modified pyrimidine nucleotides.
 16. The siNA molecule of claim 4, wherein said antisense region comprises a phosphorothioate internucleotide linkage at the 3′ end of said antisense region.
 17. The siNA molecule of claim 4, wherein said antisense region comprises between about one and about five phosphorothioate internucleotide linkages at the 5′ end of said antisense region.
 18. The siNA molecule of claim 10, wherein said 3′-terminal nucleotide overhangs comprise ribonucleotides that are chemically-modified at a nucleic acid sugar, base, or backbone.
 19. The siNA molecule of claim 10, wherein said 3′-terminal nucleotide overhangs comprise deoxyribonucleotides that are chemically-modified at a nucleic acid sugar, base, or backbone.
 20. The siNA molecule of claim 10, wherein said 3′-terminal nucleotide overhangs comprise one or more universal base ribonucleotides.
 21. The siNA molecule of claim 10, wherein said 3′-terminal nucleotide overhangs comprise one or more acyclic nucleotides.
 22. The siNA molecule of claim 10, wherein said 3′-terminal nucleotide overhangs comprise nucleotides comprising internucleotide linkages having Formula I:

wherein each R1 and R2 is independently any nucleotide, non-nucleotide, or polynucleotide which can be naturally occurring or chemically-modified, each X and Y is independently O, S, N, alkyl, or substituted alkyl, each Z and W is independently O, S, N, alkyl, substituted alkyl, O-alkyl, S-alkyl, alkaryl, or aralkyl.
 23. The siNA molecule of claim 10, wherein said 3′-terminal nucleotide overhangs comprise nucleotides or non-nucleotides having Formula II:

wherein each R3, R4, R5, R6, R7, R8, R10, R11 and R12 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, C1, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO₂, NO₂, N3, NH_(2,) aminoalkyl, aminoacid, aminoacyl, ONH_(2,) O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I; R9 is O, S, CH2, S═O, CHF, or CF2, and B is a nucleosidic base or any other non-naturally occurring base that can be complementary or non-complementary to EGFR RNA or a non-nucleosidic base or any other non-naturally occurring universal base that can be complementary or non-complementary to EGFR RNA.
 24. The siNA molecule of claim 10, wherein said antisense region 3′-terminal nucleotide overhang comprise a 3′-terminal substituted polyalkyl moiety having Formula VII:

wherein each n is independently an integer from 1 to 12, R1, R2 and R3 is independently H, OH, alkyl, substituted alkyl, alkaryl or aralkyl, F, Cl, Br, CN, CF3, OCF3, OCN, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, SO-alkyl, alkyl-OSH, alkyl-OH, O-alkyl-OH, O-alkyl-SH, S-alkyl-OH, S-alkyl-SH, alkyl-S-alkyl, alkyl-O-alkyl, ONO2, NO2, N3, NH2, aminoalkyl, aminoacid, aminoacyl, ONH2, O-aminoalkyl, O-aminoacid, O-aminoacyl, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalklylamino, substituted silyl, or group having Formula I, and either R1, R2 or R3 serve as points of attachment to the siNA molecule of the invention.
 25. An expression vector comprising a nucleic acid sequence encoding at least one siNA molecule of claim 1 in a manner that allows expression of the nucleic acid molecule.
 26. A mammalian cell comprising an expression vector of claim
 25. 27. The mammalian cell of claim 26, wherein said mammalian cell is a human cell.
 28. The expression vector of claim 25, wherein said at least one siNA molecule comprises a sense region and an antisense region and wherein said antisense region comprises sequence complementary to an RNA sequence encoding EGFR and the sense region comprises sequence complementary to the antisense region.
 29. The expression vector of claim 28, wherein said at least one siNA molecule comprises two distinct strands having complementarity sense and antisense regions.
 30. The expression vector of claim 28, wherein said at least one siNA molecule comprises a single-strand having complementary sense and antisense regions.
 31. The siNA molecule of claim 4, wherein any pyrimidine nucleotides present in said sense region comprise 2′-deoxy-2′-fluoro pyrimidine nucleotides and wherein any purine nucleotides present in said sense region comprise 2′-deoxy purine nucleotides.
 32. The siNA molecule of claim 31, wherein any nucleotides comprising a 3′-terminal nucleotide overhang that are present in said sense region are 2′-deoxy nucleotides.
 33. The siNA molecule of claim 4, wherein any pyrimidine nucleotides present in said antisense region comprise 2′-deoxy-2′-fluoro pyrimidine nucleotides and wherein any purine nucleotides present in said antisense region comprise 2′-O-methyl purine nucleotides.
 34. The siNA molecule of claim 33, wherein any nucleotides comprising a 3′-terminal nucleotide overhang that are present in said antisense region are 2′-deoxy nucleotides. 